guia de operacion biocomand

8/16/2019 Guia de Operacion Biocomand

1/205


2/205

ii

BioCommand Plus M1291-0050 User’s Guide


3/205

iii

New Brunswick Scientific User’s Guide

INTERNATIONAL OFFICES:

THE NETHERLANDS New Brunswick Scientific BV

Kerkenbos 1101, 6546 BC Nijmegen

P.O Box 6826, 6503 GH Nijmegen

Holland

Tel: (0)24 3717 600Fax: (0)24 3717 640

E-mail: [email protected]

BELGIUM New Brunswick Scientific NV/SA

‘t Veldeke, 1

B-1970 Wezembeek-Oppem

België/Belgique

Tel: (0)2 731 6787Fax: (0)2 731 8130


FRANCE

New Brunswick Scientific SARL

3, rue des Deux-Boules

75001 Paris

France Tel: (0)1 40 26 47 06

Fax: (0)1 40 26 54 23


GERMANY

New Brunswick Scientific GmbH

In Der Au 14

D-72622 Nürtingen

Deutschland Tel: (0)7022 932490

Fax: (0)7022 32486


UNITED KINGDOM

New Brunswick Scientific (UK) Ltd.

Edison House, 163 Dixons Hill Road North Mymms, Hatfield, Herts. AL9 7JE

United Kingdom

Tel: (0)1707 275733Fax: (0)1707 267859


CHINA (Beijing)

Room 8-3-B

No. 1 Heng Fu Zhong Street

Feng Tai District

Beijing 100070

P.R. China

Tel: 10 6371 0167

Fax: 10 6374 1107

CHINA (Shanghai)

Room 1406, Aijian No. 1 Bldg.

Lane 590, WanPing (S) Road

Shanghai 200030

P.R. China

Tel: 21 6481 2658

Fax: 21 6481 2665



4/205

iv


THIS PAGE INTENTIONALLY BLANK


5/205

v


CAUTION! This equipment must be operated as described in thismanual. If operational guidelines are not followed,equipment damage and personal injury can occur.Please read the entire User’s Guide before attempting touse this unit.

Do not use this equipment in a hazardous atmosphere orwith hazardous materials for which the equipment was notdesigned.

New Brunswick Scientific Co., Inc. (NBS) is not responsiblefor any damage to this equipment that may result from theuse of an accessory not manufactured by NBS.


6/205

vi




7/205

vii


Copyright NoticeNew Brunswick Scientific Company, Inc.Box 4005 44 Talmadge RoadEdison, New Jersey 08818-4005

Copyright 2002 New Brunswick Scientific Co., Inc.

All Rights Reserved.Reproduction, adaptation, or translation without prior writtenpermission fromNew Brunswick Scientific is prohibited.

Disclaimer Notice New Brunswick Scientific Co., Inc. reserves the right to changeinformation in this document without notice. Updates to informationin this document reflect our commitment to continuing product

development and improvement.

Manual Conventions

NOTE:

CAUTION!

WARNING!

Bold Text in bold face type emphasizes key wordsor phrases.

Caution messages appear before procedures

which, if caution is not observed, could resultin damage to the equipment.

Notes contain essential information thatdeserves special attention.

Warning messages alert you to specificprocedures or practices which, if not followedcorrectly, could result in serious personal injury.


8/205

viii




9/205

ix


Every Instrument manufactured by the

New Brunswick Scientific Co., Inc. is warranted to be free from

defects in material and workmanship. This apparatus, with the

exception of glassware, lamps and electrodes (where supplied),

is warranted for 1 year against faulty components and assembly and our obligation under this warranty is limited to repairing or replacing

the instrument or part thereof, which shall, within 1 year after date of

shipment, prove to be defective after our examination. This warranty

does not extend to any NBS products which have been subjected

to misuse, neglect, accident or improper installation or

application; nor shall it extend to products which have been

repaired or altered outside the NBS factory

without prior authorization from

New Brunswick Scientific Co., Inc.

W A R R A N T Y


10/205

x




11/205

xi


TABLE OF CONTENTS

1 READ THIS FIRST ..................................................................................1

1.1

Before Installing BioCommand Plus................................................................................................ 1 1.2 Obtaining a Product Registration Number ....................................................................................... 1

1.3 License Agreement Acceptance....................................................................................................... 2

2 INTRODUCTION .....................................................................................5

2.1 Major Features ................................................................................................................................. 5 2.2 Product Benefits............................................................................................................................... 5 2.3 How to Use This Manual ................................................................................................................. 6 2.4 Organization of This Manual ........................................................................................................... 7 2.5 Notation Conventions Used In This Manual.................................................................................... 7 2.6 Navigating Through Windows ....................................................................................................... 8 2.7 Reference for Item Naming Conventions....................................................................................... 10

3 B IOC OMMAND P LUS INSTALLATION & SET-UP............................................11

3.1 Workstation Setup.......................................................................................................................... 11 3.2 Starting BioCommand Plus............................................................................................................ 14 3.3 Workgroup Information ................................................................................................................. 14 3.4 Recipes and Batches....................................................................................................................... 15 3.5 “Starting BioCommand” Screen .................................................................................................... 15 3.6 Main Menu..................................................................................................................................... 16 3.7 Main Toolbar.................................................................................................................................. 17

4 NBS HARDWARE CONNECTION SETUP ......................................................19

4.1 Cable Connections ......................................................................................................................... 19 4.2 Setting Up Computer/Controller(s) Communication ..................................................................... 20

5 CREATING A RECIPE & STARTING A BATCH.................................................27

5.1 Creating a New Recipe using Batch Wizard .................................................................................. 27 5.2 Expanding a Basic Recipe.............................................................................................................. 33 5.3 Alternative Methods to Create a New Recipe ............................................................................... 34 5.4 Starting A Batch............................................................................................................................. 40 5.5 Batch Summary View .................................................................................................................... 44

6 INTRODUCTION TO BATCH VIEWS.............................................................47

6.1 Trend Views................................................................................................................................... 47 6.2 Using the Trend Graph................................................................................................................... 50 6.3 Creating Synoptic Views................................................................................................................ 51

7 CREATING PROGRAMS ..........................................................................57

7.1 How to Create a Program............................................................................................................... 58 7.2 User-Defined Loops....................................................................................................................... 68 7.3 More Program Functions................................................................................................................ 69


12/205

xii


8 SETTING ALARMS & ALERTS ..................................................................71

8.1 Setting Alarms................................................................................................................................ 71 8.2 Setting Alerts.................................................................................................................................. 75

9 ENDING A BATCH ................................................................................77

9.1 End a Batch .................................................................................................................................... 77 9.2 Exiting BioCommand Plus............................................................................................................. 77 9.3 Restarting with Active Batches...................................................................................................... 78 9.4 Shutting Down BioCommand Plus ................................................................................................ 79

10 SECURITY FEATURES ............................................................................81

10.1 User-Based Access .................................................................................................................... 81 10.2 Entering User Passwords........................................................................................................... 81 10.3 Recipe Authorization Levels ..................................................................................................... 85 10.4 Creating and Modifying a User List .......................................................................................... 85

11 MAIN SCREEN FUNCTIONS REFERENCE.......................................................91

11.1 File............................................................................................................................................. 91 11.2 Edit ............................................................................................................................................ 92 11.3 View .......................................................................................................................................... 93 11.4 Tools.......................................................................................................................................... 94 11.5 User ........................................................................................................................................... 98 11.6 Window..................................................................................................................................... 99 11.7 Help ......................................................................................................................................... 101 11.8 Toolbar Description................................................................................................................. 101

12 RECIPE/BATCH FUNCTIONS ..................................................................103

12.1 Creating or Editing Recipes..................................................................................................... 103 12.2 Starting a Batch ....................................................................................................................... 121 12.3 Altering a Batch in Progress.................................................................................................... 122

13 CREATING & EDITING VIEWS ................................................................131

13.1 AutoRefresh Feature................................................................................................................ 131 13.2 Program Views........................................................................................................................ 133 13.3 Synoptic Views........................................................................................................................ 146 13.4 Trend Views ............................................................................................................................ 150

14 TROUBLESHOOTING............................................................................ 159

14.1 Troubleshooting Guide............................................................................................................ 159

15 SPECIFICATIONS & SYSTEM REQUIREMENTS ............................................. 161

15.1 BioCommand Plus Specifications........................................................................................... 161 15.2 System Requirements.............................................................................................................. 161 15.3 Network & Communications Requirements............................................................................ 162 15.4 Fermentor/Bioreactor Installation............................................................................................ 162 15.5 Creating a BioCommand Plus Workgroup .............................................................................. 162


13/205

xiii


16 DATA LOG FORMAT ........................................................................... 165

16.1 Settings.................................................................................................................................... 166 16.2 Changes ................................................................................................................................... 167 16.3 Batch Events............................................................................................................................ 169 16.4 Reports .................................................................................................................................... 169

16.5 Data Logs ................................................................................................................................ 170 16.6 Batch Identifications................................................................................................................ 172 16.7 User Data................................................................................................................................. 174 16.8 Errors....................................................................................................................................... 174 16.9 Views Data .............................................................................................................................. 175

17 VIEWING BATCH DATA .......................................................................177

17.1 Starting DBViewer32.............................................................................................................. 177 17.2 Existing Batches...................................................................................................................... 177 17.3 Using the Main Data Window ................................................................................................. 178 17.4 More Batches........................................................................................................................... 187

18 INDEX ............................................................................................ 189


14/205

xiv

_____________________________________________________________________________BioCommand Plus User’s Guide



15/205

1


11 RREEAADD TTHHIISS FFIIRRSSTT

1.1 Before Installing BioCommand Plus

BioCommand Plus is copyrighted software and may not be used without a license.

Before you do anything else, read this entire chapter.

The license supplied with your purchase is good for a networked installation at one single

site. Before BioCommand Plus can be used, you will need to obtain a Product Registration Number from New Brunswick Scientific.

NOTE:From the time you receive your Product Registration Number, you may useit to activate BioCommand Plus during a period of two weeks. If you arenot ready to install and activate the software, do not request the productRegistration Number at this time.

The number of Product Registration Numbers that New Brunswick Scientific will issue

against one BioCommand Plus serial number is limited. Be sure you are ready to proceedwith installation before you request a Product Registration Number. Also, be sure to save

the BioCommand Plus serial number in case reinstallation becomes necessary, because a

Product Registration Number cannot be issued without the serial number.

1.2 Obtaining a Product Registration Number

The Product Registration Number can be obtained in several ways:

• Online through the worldwide web (the fastest and easiest method to use in most cases)

• Through your local NBS sales office, by e-mail, phone or fax

• Through our U.S. headquarters, by e-mail, phone or fax

1.2.1 Online Registration

Contact this website: http://www.nbsc.com

Fill out the online Registration Form. You will be asked for:

• your name and title


16/205

2


• your “Institution” (the company, institution or organization where youwork)

• your institution’s street address, city, state or province, and country

• your work phone number and e-mail address

• the serial number of your BioCommand Plus software, which is found on

the back of the CD case.

1.2.2 Registration at Local NBS Sales Office

Contact your local NBS sales office by telephone, fax or e-mail, and provide

the information listed in Section 1.2.1.

1.2.3 Registration at NBS U.S. Office

Contact NBS headquarters:

• by telephone: 1-732-287-1200

• by fax: 1-732-4222

• by e-mail: [email protected]

to provide the information listed in Section 1.2.1.

1.3 License Agreement Acceptance

You must also accept the License Agreement below before you can use this software.

NEW BRUNSWICK SCIENTIFIC CO., INC.

LICENSE AGREEMENT FOR BIOCOMMAND PLUS

This is a legal agreement between you, the end user, and New Brunswick Scientific Co., Inc.

By obtaining an installation password and installing this software, you are agreeing to be

bound by the terms of this agreement.

If you do not agree to the terms of this agreement, promptly return the package and the

accompanying items to New Brunswick Scientific.

1.3.1 New Brunswick Scientific Software License

1. Grant Of License

New Brunswick Scientific grants you the right to use the BioCommand Plus software program ( BioCommand Plus) in a networked environment at a single site. You may


17/205

3


install BioCommand Plus on multiple computers within a single network domain.

You may not install this copy of BioCommand Plus in more than one network domain

at the same time.

2. Copyright

The BioCommand Plus program is owned by New Brunswick Scientific or its suppliers

and is protected by United States copyright laws and international treaty provisions.

Therefore, you must treat BioCommand Plus like any other copyrighted material (e.g.,a book or musical recording) except that you may make one copy of BioCommand Plus

solely for backup or archival purposes.

3. Other Restrictions

You may not rent, lease or transfer BioCommand Plus. You may not reverse engineer,

decompile, or disassemble BioCommand Plus.

1.3.2 U.S. Government Restricted Rights

The BioCommand Plus software program and documentation are provided with

RESTRICTED RIGHTS. Use, duplication, or disclosure by the Government is subjectto restrictions as set forth in subdivision (b)(3)(ii) of The Rights in Technical Data and

Computer Software clause at 252.227-7013. Contractor/manufacturer is New Brunswick

Scientific Co., Inc./44 Talmadge Road/P. O. Box 4005/Edison, New Jersey 08818-4005.

This Agreement is governed by the laws of the State of New Jersey. Should you haveany questions concerning this Agreement, or if you desire to contact New BrunswickScientific for any reason, please write: New Brunswick Scientific Customer Sales and

Service, 44 Talmadge Road, P.O. Box 4005, Edison, New Jersey 08818-4005.


18/205

4




19/205

5


22 IINNTTRROODDUUCCTTIIOONN

BioCommand Plus software enhances the capabilities of NBS

bioprocessing equipment. It oversees the independent operation of one ormany NBS process controllers. BioCommand Plus enables centralized

control, monitoring and data acquisition in applications ranging from a single benchtop laboratory fermentor through arrays of large-scale

production vessels. This software uses industry-standard conventions,

including the familiar terms of Recipe (all of the variables associated witha fermentation run) and Batch (an active application of a recipe).

2.1 Major Features

• Centralized monitoring and control of one or several fermentors and bioreactors

• Network connectivity, so several computer stations can serve as observation andcontrol centers

• Automatic process data recording

• Clear synoptic, graphical, pictorial and numerical displays and reports

• User programming for automating research and production protocols

• Security features

2.2 Product Benefits

2.2.1 General

BioCommand Plus is a Windows –based, network-capable, multi–bioprocess

supervision program. It is designed primarily for use with New Brunswick

Scientific’s fermentation and cell culture equipment, but can be extended to the

control and supervision of other equipment as well. This software uniquelyretains the easy-to-use character of its precursor, AFS BioCommand, while

adding powerful networking; security; 32–bit architecture; expanded

programming, enhanced graphing and reporting capabilities; and recipe-based process control.

We are confident that that the power, flexibility and convenience of this software

will enhance creativity and productivity in laboratory, pilot, and manufacturing

applications.


20/205


21/205

7


Follow the first three chapters carefully to ensure that the software is installed

correctly, and the appropriate interface connects the controlled instrument(s) to the

host computer(s). If you plan to network your installation, you may need the

assistance of networking personnel.

Refer to Chapters 3-8 to set up and complete a first BioCommand Plus run.As needed, refer to the remaining chapters.

2.4 Organization of This Manual

Chapters 1-2 Introductory material.

Chapter 3-8 These chapters take the new user from program start-up

through fermentor shutdown in step-by-step tutorial style.They include sufficient detail to enable further independent

exploration.

Chapters 9-12 Reference chapters containing in-depth explanations of

features.

Chapter 13 A troubleshooting guide to help you diagnose and resolve some

potential difficulties.

Chapters 14-18 Information that is usually not necessary for routine programoperation (product specifications, system requirements,

installation information, data log formats and hardware

connection instructions), but which may be helpful to advancedusers.

Chapter 19 An index to key terms used throughout the manual.

2.5 Notation Conventions Used In This Manual

2.5.1 Mouse Notation

Notation Application

Click Press the left mouse button once.

Double Click Press the left mouse button twice, in quick

succession.

Right Click Press the right mouse button once.

Double Right Click Press the right mouse button twice, in quicksuccession.


22/205

8


2.5.2 Keyboard Notation

Keys are displayed with their keyboard label between the “” symbols,such as (Escape) or .

Modifier keys (, , ) which are pressed along with another keyare also shown between the two symbols < and >. The modifier is separated from

the other key by a dash. For example, means that both (theControl key, which is labeled Ctrl) and (the key labeled D) must be pressed

simultaneously.

If modifier key(s) are required, press and hold down the modifier(s) while

pressing and releasing the other key. Then release the modifier key(s).

If two modifier keys are required both are shown within the symbols < >, and areseparated by dashes, as .

Function keys are displayed with an “F” preceding a number: .

2.5.3 Menu Notation

Menu items are described by the selection sequence that accesses the item. A

dash separates items in the sequence. For example, the Save function under the

File menu is written as File – Save.

2.5.4 Window Element Notation

• Window names (in the window title bar) are enclosed in double quotation marks,as “Loop Setup Options”.

• Tab labels are also enclosed in double quotation marks: “Recipes”.

• Data-entry-field names use single quotation marks, as in ‘Loop’.

• On-screen (virtual) Button captions are enclosed in curly brackets “{ }”.

2.6 Navigating Through Windows

Skip this section if you are very familiar with the Windows

Operating System. Those

in need of a review should read on. For more information, consult Microsoft or third

party manuals on the use of your operating system.

• The Close Button – this button () exits the program.

• The Maximize/Restore Button – the active window can be set to occupy the entire

screen (maximized) or just a portion of the screen with this button (). When thewindow is not maximized, it can be resized with the following steps:


23/205

9


1. Place the cursor over one of the edges or at the corner of the window until the

cursor changes to a double arrowhead.

2. Click the left mouse button and drag the cursor in or out to reduce or enlarge the

window.

• The Minimize Button –this button () reduces the program window to an icon on thetask bar on the bottom of the screen.

See the sample screen below to locate the Close, Maximize/Restore and Minimize

buttons.

• Shortcut keys are indicated by an underlined character in a menu item.. From the

Main Menu, press the key along with the shortcut key to open that menu item.While the menu is active, pressing the key associated with the underlined character

is the same as clicking on that menu item. Most of the selection buttons that are

used throughout this program also include shortcut keys.

•Many of the windows throughout this program consist of tabbed notebooks. Whenthe window is first opened, one page of the notebook will be active. The rest of the

pages will be hidden, but their tabs will be visible at the top or bottom of the

window. To change pages in these notebooks, click on the tab for the page youwant; that page will become the active screen.

Minimize

Maximize/Restore

Close


24/205

10


2.7 Reference for Item Naming Conventions

The BioCommand Plus program uses a system of naming items that is described in thissection. Once a display name is given to a recipe or a batch, that name is used to

identify all items belonging to that recipe or batch. The format for these items is:

display name.Bx:item name.y

where

display name is the name given to the recipe or batch

x is the number of the batch being used

item name is the functional name for the item y is the number of the instance of this item

2.7.1 File Naming Conventions

File Name

Extension:

Function:

.RCP Recipe Files(contain all information necessary to run a batch)

.BDB Batch Files

(contain all logged data, including alerts andalarms and, where appropriate, offline data)

.MDW User Workgroup identification files

(indicating recognized Users and their security

levels)


25/205

11


33 B B I I OOC C OOM M M M A AN N DD P P LLU U S S IINNSSTTAALLLLAATTIIOONN &&

SSEETT--UUPP This section guides you through initial program installation, configuration and

startup. This chapter also introduces and defines the terms “recipe” and “batch”.

For detailed system requirements and instructions on setting up BioCommand Plus

workgroups, consult Section 15.

3.1 Workstation Setup

The following subsections provide step-by-step instructions for workstations running

Windows

98, NT, 2000 or ME. The Windows

2000 instructions are applicable to

Windows

XP and Windows

XP Pro. Determine which applies to your system, and

follow the bulleted instruction sequence for that configuration.

3.1.1 Windows 98 Stand-Alone

NOTE:If you do not know your number, visit the NBS website (www.nbsc.com) toregister your software and to obtain the Product Registration Number.

1. Install BioCommand Plus:

a. Start your computer in Windows

98.

b. Close all programs, including virus detectors, desktop managers and

application toolbars.c. Insert the CD-ROM in the appropriate drive.

d. You will be prompted to “Please enter the Product Registration Number”.

Enter the registration number and click on OK.e. Follow the on-screen instructions to run the installation process. Default

values are recommended for file locations.


26/205

12


2. NBSHardwareConnection is a program that sets up communications between

the computer and controller(s). Before running BioCommand Plus for the firsttime, or whenever attached controllers are changed, run

NBSHardwareConnection as explained in Section 4.

3. Start BioCommand Plus and verify communication with connected

fermentors.

3.1.2 Windows 98 Workstation on a Windows NT Network


1. Install Microsoft remote registry service.

2. Enable remote access to the computer in the control panel – password screen.3. Set network access mode to User-Level Access.4. Grant remote access to the registry to “the world”/everyone.



98.


application toolbars.

c. Insert the CD-ROM in the appropriate drive.d. You will be prompted to “Please enter the Product Registration Number”.

Enter the registration number and click on OK.

e. Follow the on-screen instructions to run the installation process.

Default values are recommended for file locations.

6. NBSHardwareConnection is a program that sets up communications betweenthe computer and controller(s). Before running BioCommand Plus for the first

time, or whenever attached controllers are changed, run NBSHardwareConnection as explained in Section 4.


fermentors.

3.1.3 Windows NT Workstation – Networked or Stand-Alone


1. Make sure Windows NT

service pack 5 is installed.


27/205

13



a. Start your computer in Windows NT

. Be sure to log on as a user with

Admin privileges.

b. Close all programs, including virus detectors, desktop managers andapplication toolbars.

c. Insert the CD-ROM in the appropriate drive.d. You will be prompted to “Please enter the Product Registration Number”.

Enter the registration number and click on OK.e. Follow the on-screen instructions to run the installation process.

Default values are recommended for file locations.

3. NBSHardwareConnection is a program that sets up communications between

the computer and controller(s). Before running BioCommand Plus for the first

time, or whenever attached controllers are changed, run NBSHardwareConnection as explained in Section 4.


fermentors.

3.1.4 Windows 2000, XP & XP Pro Workstation – Networked orStand-Alone


1. If you are using Windows 2000, make sure the Windows 2000 service pack5 is installed.



. Be sure to log on as a user withAdmin privileges.


application toolbars.c. Insert the CD-ROM in the appropriate drive.

d. You will be prompted to “Please enter the Product Registration Number”.Enter the registration number and click on OK

e. Follow the on-screen instructions to run the installation process.

Default values are recommended for file locations.3. NBSHardwareConnection is a program that sets up communications between

the computer and controller(s). Before running BioCommand Plus for the first

time, or whenever attached controllers are changed, run

NBSHardwareConnection as explained in Section 4.4. Start BioCommand Plus and verify communication with connected

fermentors.


28/205

14


3.2 Starting BioCommand Plus

After BioCommand Plus has been successfully installed on your computer, it may bestarted in any of the usual ways, such as:

• From the Start button: Click Start Programs NBS BioCommand Plus• From a desktop shortcut: Double click a shortcut previously placed on the desktop

(refer to a Windows

manual)

• From the Windows Explorer

: double click on “BCPLUS.exe” in the NBS folder.

3.3 Workgroup Information

The first time that the program is executed, you will be asked to enter a defaultworkgroup file name and location:

Click on {Browse} to locate it:

Then click on {OK}. The “Select BioCommand Workgroup” window will close.

NOTE:

If this installation of BioCommand Plus is not to be networked, you mayuse the file in the BioCommand Plus Install folder created by the setupprogram. Otherwise, select the workgroup file that was set up for yourBioCommand Plus workgroup. (See also “Creating a BioCommandWorkgroup”, Section 15.5)


29/205

15


3.4 Recipes and Batches

The terms Recipe and Batch appear throughout this manual.

A Recipe is a process description that BioCommand Plus uses to control a fermentation

process. Recipes are created and defined by users. A Recipe is a template that dictateshow the process will proceed. Recipes include initial setpoints for controlled parameters

such as temperature, pH, dissolved oxygen and others. Recipes may also include

programs that alter setpoints at certain times or under specified conditions. Other Recipe parameters may include display types, data log interval, alarm and limit values, types of

reports to generate, and even which fermentor operators have authority to alter the recipe

itself or the runs derived from it.

Batch refers to a specific fermentation or cell culture run. Every Batch starts from a

Recipe. The same Recipe may be used over and over to run several Batches. Once a

batch has begun, Recipe dictates may be overridden by those operators granted

appropriate authority for the batch.

3.5 “Starting BioCommand” Screen

Whenever you open BioCommand Plus, you will see the “Starting BioCommand” screenrepresented below (unless you turn it off):

This screen offers six options:

• Create a new recipe with Batch Wizard: opens the Wizard which guides you step bystep through recipe creation.


30/205

16


• Open a blank recipe: once you are familiar with BioCommand Plus, you can use thisshortcut to create a recipe, without the Wizard.

• Open an existing recipe: one of several ways to access existing recipes.

• Extract a recipe from a batch: this use of the Batch Wizard will allow you to create arecipe used from a previous batch’s archive file.

• View a batch running elsewhere on the network : this option allows you to look at any batch that may be running on your network.

• Resume a batch running when BioCommand exited: whether it was a power failure, a

PC failure or user error, even though BioCommand Plus was not properly shut down, this

command will allow you to resume computer supervision of an active batch.

3.5.1 Turning the “Starting BioCommand” Screen Off & On

To prevent the “Starting BioCommand” screen from appearing in future runs,click in the white checkbox (“Don’t show this display on start up”), then close the

window.

You can turn it back on by selecting File – Start Options from the Main Menu.The “Starting BioCommand” screen will open. Click in the box to remove the

checkmark, then close the window (or continue if you wish to use the Wizard).

3.6 Main Menu

The menu items on the main BioCommand Plus screen comprise the Main Menu. Below the

Main Menu is the optional Toolbar. Many of the most commonly used functions that are

available from the Main Menu or its submenus are available by clicking on the appropriateicon on this toolbar. When the cursor is placed over an icon, a one- or two-word hint

describing the function of that icon is displayed for a short time in a small box beneath the

icon.


31/205

17


This is the appearance of the Main Screen, under the “Starting BioCommand” screen, when

the program is started.

3.7 Main Toolbar

The Toolbar may be toggled off from the View dropdown menu if more screen area for

program output display is desired. The figure below indicates the function of each icon on

the Toolbar.

What’s This? HELPBatch Wizard

View Recipe

New Recipe

View Batch

New Batch

Copy

Paste

Delete

Save

Print

Cut

Standard Windows ®

functions


32/205

18




33/205

19


44 NNBBSS HHAARRDDWWAARREE CCOONNNNEECCTTIIOONN

SSEETTUUPP This setup program is required to allow your computer, BioCommand Plus , and all

controllers and fermentors in your system to communicate with each other. This program must be run before you attempt to use BioCommand Plus.

4.1 Cable Connections

Before you run this set-up program, you must make sure that your hardware is

properly cabled.

4.1.1 Required Hardware

The NBS Multi-Controller Adapter (#M1286-0100) and NBS RS-422 cable(#M1171-8010) included with BioCommand Plus provides communication using

AFS protocol with NBS controller(s), which may be any one or combination of

the following:

• BioFlo 110 Primary Control Unit • BioFlo 5000

• BioFlo 2000 • CelliGen Plus

•

BioFlo III•

Mobile Pilot Plant• BioFlo 3000 • ML-4100 controller

• BioFlo IV • ML-6100 controller

• BioFlo 4000 • 4-20 mA controller

• General Purpose controller

You will need one RS-422 cable per supervised fermentor.

4.1.2 Making Connections

1. Connect the 25-pin connector of an NBS RS-422 cable (#M1171-8010) to the

AFS serial port of one controller.2. Connect the cable end of the NBS Multi-Controller Adapter (#M1286-0100)

to the other (far) end of the NBS RS-422 cable.

3. Connect the adapter end marked “RS-232” to the serial input port of the

computer.4. If you are using a BioFlo 110 PCU or an ML-6100, make sure the controller is

set to AFS protocol. Consult the controller user guide for instructions. All

other NBS controllers are factory-set to AFS protocol.


34/205

20


5. Additional controllers may be connected using additional RS-422 cables to

daisy chain from the first controller to the second, and so on.

BioCommand Plus also works with the NBS Interface (see interface box manual

for details).

4.2 Setting Up Computer/Controller(s) Communication

NOTE: Follow the instructions in this section carefully to ensure thatBioCommand Plus communicates with your hardware.

In order for BioCommand Plus to share information with each of the attached controllers,

you must provide three essential pieces of information to the software:

• type of connection (direct/multi-fermentor adapter or NBS BioCommandInterface)

• which computer port is connected to the controller(s)

• the unique “ID number” of each controller.

Use the NBS Hardware Connection Setup program to enter this information.

4.2.1 NBS Hardware Connection Setup

1. Open NBS Hardware Connection Setup by clicking, in succession, on Start Programs NBS NBSHardwareConnection

2. The program window opens:

3. A few seconds later, the Controller Connection Type window opens:


35/205

21


4. Click on one option button ():

• NBS Controller(s) using direct-to-PC connection or the NBS Multi-

Fermentor Adapter. Choose this option if your controller is connectedeither (a) via cable directly to the Comm port of your computer, or (b) via

cable to the NBS Multi-Fermentor Adapter (NBS kit number M1291-

8000), which in turn is connected to the Comm port of your computer.

• NBS Controller(s) via NBS BioCommand Interface. Choose thisoption if you have installed an NBS BioCommand Interface between the

fermentor(s) and the Comm port of your computer.

NOTE: Anytime you change connection type, to protect you from operator errorthe following warning screen will open:

Click on {Yes} to apply the change or {No} to cancel it.

5. Click on {Next >>} to open the Computer Communications window (see

Section 4.2.2).


36/205

22


4.2.2 Select Comm Port

1. In the “Select Comm Port” pane, indicate which computer Comm port, 1 or 2,is connected to your controller(s).

2. In the “Comm Settings” pane, the correct choices are preset for you. Do not

change any of these settings.

3. Click on the {Open Comm Port} button to test the selected Comm port.

4. If you get an error message, it most likely means you have incorrectlyidentified the port connected to your controller(s). Change the “Select Comm

Port” setting, then click again on the {Open Comm Port} button.

5. When the test message indicates no error, click {Next>>} to open the

Controller ID List window (see Section 4.2.3).

4.2.3 Controller ID List

In the Controller ID List, you may enter up to eight “ID” (identification) numbersfor the controller(s) attached to your computer. ID numbers are electronic“names” that allow the software to communicate individually with each of the

attached controllers.

Click here tochange thenumber

DO NOTCHANGE!


37/205

23


NOTE: • ID numbers have nothing to do with product, model or serial numbers.

• The provided Multi-Controller Adapter or an NBS BioCommand Interfaceis required to use more than one controller with BioCommand Plus.

Refer to your fermentor, bioreactor or controller User Guide to learn how to view

the ID number for that specific instrument ( see Sample Retrieval of ID Numbers

on the following page). In some older NBS controllers, ID numbers are referredto as “MD” or “multidrop” numbers.

Sample Retrieval of ID Numbers

To obtain the ID numbers for the BioFlo 110, for example:

1. Press the upper right BioFlo 110 touchpad key to display the Setup Screen:


38/205

24


English

FrançaisDeutsch

Español

Use…

Setu

p

More…

2. Press the More… selector button to display the Systems Detail screen:

System DetailsUnit Base Address: 0 BaseAdd

Unit 1 ID: 0

Unit 2 ID: 1

Unit 3 ID: 2

Unit 4 ID: 3

Comm. Protocol: AFS Comm

Setup

Recorder Status: Off Recorder

3. Note the four Unit ID number(s).

4. If you have only one vessel, use only the first ID number. Enter that numberin field 1 of the Controller ID List.

5. If you have more than one vessel, enter additional numbers in subsequentfields of the Controller ID List.

End of Sample Retrieval.

When you have entered the ID numbers in the Controller ID List, click on

{Next >>}. Within a few moments, the Controller Status window opens.

NBS BioFlo 110Version : 0001.01

Config : 1.10

Date: 2/2000

http://www.nbsc.com

SELECTOR BUTTONS


39/205

25


4.2.4 Controller Status

The Controller Status window indicates the ON/OFF status and available loops ineach controller.

NOTE: If the indicated status does not agree with what is observed at the

controller(s), click {>}to return to this screen and recheck the status. If the status is still notcorrect, check all cable connections, and confirm the operation of eachcontroller.

When you have confirmed the correct controller status, click {Finish} to exit NBSHardware Connection.

Clickhere tocheckentireloop lists


40/205

26




41/205

27


55 CCRREEAATTIINNGG AA RREECCIIPPEE && SSTTAARRTTIINNGG AA

BBAATTCCHH

Follow the steps in this chapter to create a new Recipe (with or withoutthe help of Batch Wizard), start a Batch from that Recipe, and view the

Batch status using a Batch Summary display. The Recipe, Batch, and

Display in this chapter are “minimalist” in that they do not invoke themost powerful or impressive BioCommand Plus capabilities.

Nevertheless, performing the steps in this tutorial will impart a feel to the

new user for how the software works and what it does.

To start a Batch you must either specify a previously saved Recipe or create a new one.

A Recipe must contain at least the following information:

• A data recording interval

• A data log file name

• A list of parameters (loops) identified by name and the controller in which theyreside.

Recipes usually contain more than the minimum required information. The Recipe created in

this section is minimal, but it will be expanded in later chapters. The easiest way to create a

recipe is to use the Batch Wizard; other methods are discussed in Section 5.3.

5.1 Creating a New Recipe using Batch Wizard

In the “Starting BioCommand” screen (see page 15):

1. Click on the white option button next to Create a new recipe with Batch Wizard (if it isnot already selected)

2. Click on {Continue} or, if you change your mind, Click on {Close} to exit Batch

Wizard.


42/205

28


If you choose {Continue}, the “BioCommand Batch Wizard Introduction” screen

opens:

Notice, too, that the New Recipe Setup screen will also open behind it. You will have

access to the Setup screens when you have finished Batch Wizard.

If you do not wish to see this introduction screen when you use Batch Wizard again, click

in the white checkbox next to Skip this screen in the future.

You may use any of the active buttons at the bottom of the screen to obtain help, to cancel

use of the wizard, to move back to the previous screen or to move ahead to the next. If a button is gray, it is inactive for that screen.

3. Click on {Next>} to move ahead. The “BioCommand Batch Wizard – Step 1” screenwill appear:


43/205

29


The default name for the recipe, which appears in the edit box, is Recipe1.

4. If you wish to keep this name, simply click on {Next>}. This is the name that willappear on the bottom Recipe tab in the Setup view when you finish Batch Wizard. It will

also appear in the title bar of all windows associated with this recipe.

5. If you wish to rename the recipe, enter the new name in the edit box. You may use up to

12 alphanumeric characters, including spaces. Then click on {Next>}. The

“BioCommand Batch Wizard – Step 2” screen will appear:


44/205

30


6. Click on the {Select Loops>>} button. The “Add/Change Hardware Loop List” windowwill appear:

7. Click on the {Browse Local Server} button. Wait a moment while BioCommand Plus

searches. The available controller(s) will appear in the left “Available Loops” pane.

8. Click on the + next to the controller(s) of interest to see the drop-down list of available

loops.

9. If you wish to select all loops from a controller, click on the controller name, then click

on the {>>} button. The controller name (and all its associated loops) will move into the

right, “Loops in Batch” pane.

10. If you wish to select one loop at a time, click on the loop name, then click on the {>>}

button. The loop name will move into the “Loops in Batch” pane.

11. When your selections are complete, click on {Close}. You will return to the Step 2Batch Wizard screen.

12. Click on {Next>}. The “BioCommand Batch Wizard – Step 3” screen will appear:


45/205

31


You must assign a ‘Recipe File Name’, which is both a file name and its folder location.

Note that every recipe must be assigned the extension .RCP which marks it as a recipe.

13. Click on the {Change Directory/Name} button.

14. The “BioCommand Save Files” window will open:

15. If this recipe should be available to your network, click now in the check box next to

Shared over the network .

16. To see existing File Name/Folder strings, click on the down arrow () to the right of the

‘File’ edit box.

17. Click on an existing File Name/Folder string to bring it into the edit box as a pattern.

You can type over it to create your new name. This will not affect the existing File Namein any way.


46/205

32


18. If you wish to find other folders, click on the {Browse} button. The screen that appears

will allow you to explore possibilities (in network if shared, or in your own PC if not), just as you would with any other Windows

® -based exploration tool.

19. Create a new one based on this example. Be sure to follow the pattern:

Drive:\FolderName\FileName.RCP (the example shown on the Step 3 screen above isC:\BCPLUS\BioRcp2.RCP). Normal Windows®

file-naming conventions apply.

NOTE:All recipe file names must have the extension “.RCP”.

• Click on {OK}. Your selection will move into the ‘Recipe File name’ edit box.

NOTE:When a recipe will be shared over the network, the file name must followthe pattern: \\ComputerName\FolderName\FileName.RCP

20. Click on {Next>}. The “BioCommand Batch Wizard – Step 4” screen will appear:

21. After you have read this screen, click on {Next>}. The “BioCommand Batch Wizard –Finished!” screen will appear:


47/205

33


22. Click on {Finish}. You will now be in the Setup View for the Recipe you have just

created.

At this point, you have a very basic recipe that is sufficient to start a batch. If you are

ready to do so, see Section 5.4, Starting a Batch, for further instructions. If you wish to

add any or all of the additional features mentioned in Step 4 of the Batch Wizard, seeSection 5.2, Expanding a Basic Recipe, below.

5.2 Expanding a Basic Recipe

At any time (before you start a batch or while a batch is running), you can expand arecipe using any or all of the features below, which are also mentioned in Step 4 of the

Batch Wizard:

• Change the logging interval See Section 12.1.4, Time Data Pane

• Set the Elapsed Fermentation Time format See Section 12.1.4, Time Data Pane

• Add Alarm and Alert settings See Section 8

• Select parameters for trend views See Section 6.1

• Design synoptic views See Section 6.3

•Set up control programs

See Section 7

• Set security access levels for other users See Section 10

• Modify the hardware loop list See Sections 5.3.2 and 12.1.5


48/205

34


5.3 Alternative Methods to Create a New Recipe

There are a number of ways to create a recipe. The easiest is to use the Batch Wizard, asoutlined in Section 5.1. You can also create the recipe without the Wizard, directly from

the “New Recipe Startup” screen. There are several ways to access the Wizard, and

several other ways to access the “New Recipe Startup” screen.

5.3.1 How to Access Batch Wizard

There are two ways to open the Batch Wizard:

• On start-up, from the “Starting BioCommand” screen, click on Create a new

recipe with Batch Wizard

• From the Main menu, click on the Batch Wizard icon.

5.3.2 How to Access the New Recipe Startup Screen

There are three ways to open the “New Recipe Startup” screen:

• From the “Starting BioCommand” screen , click on Open a blank recipe, then

click on {Continue}:

• Select File - New Recipe from the Main Menu.

• Click on the New Recipe icon on the toolbar.


49/205

35


After any of the above actions, the “New Recipe Setup” screen will appear:

1. Click in the ‘Display (Tab) Name:’ edit box and type the display name for this recipe.

The name may be any combination of up to 12 alphanumeric characters and spaces.

The ‘Owner’ name is assigned to the logged-on user creating this Recipe and may not be changed.

2. Click inside the ‘Description:’ edit box and enter descriptive text as desired. ThisDescription may be any combination of alphanumerics, spaces, and punctuation

marks.


50/205

36


3. The default Log Interval is one minute. To change the interval, click the {ChangeInterval} button in the ‘Time Data’ pane. Enter the desired Log Interval in whole

minutes in the edit box, or choose a preset interval from the dropdown list.

4. Select the Time Display Format, either HH:MM:SS (Hours : Minutes : Seconds) or

Decimal Hours, by clicking in the appropriate circle. Decimal format is convenientfor mathematical processing.


51/205

37


5. Click on the “Loops” tab. The “Add/Change Hardware Loop List” window appears.

This window allows you to select loops from any of the attached controllers. Usuallyall the loops from a single fermentor are selected. You may choose to include loops

from ancillary equipment, such as a gas analyzer or scale.

6. Click the {Browse Local Server} button. Controllers that are accessible to

BioCommand Plus appear in the field labeled “Available Loops”. In the sample

screeen below, loops from two local controllers are available for this recipe:

7. To display the individual loops associated with each controller, click on the “+” boxto the left of the controller name. The list expands as shown below:


52/205

38


8. Pressing the {>>} button moves selected loops from the “Available Loops” pane to

the “Loops in Batch” pane. Click on a controller name and then click on the{>>}

button to include all the loops from that controller in your recipe, or select anindividual loop from the expanded list and click on the {>>} button to include that

loop in your recipe. Continue until all desired loops are in the right pane of this

window.

9. Use the {


53/205

39


11. The “Current Loops” pane shows the selected loops. On the left of each line is the

loop name as transmitted to BioCommand Plus by the controller. On the right iscomplete loop data in the format [Server Name\Controller Name\Loop Name].

You may change any default loop name to a display name containing a maximum of 10

characters. You may also enter an initial setpoint for each loop in the Recipe. Both ofthese options are executed through the “Loop Setup Options” window.

12. Click on the {View loop details} button. The Loop Setup Options window will open:

A. Select the loop from the pull-down menu.

B. Enter a new display name, if desired.

C. Enter a setpoint, if desired.D. Click {Apply}.

E. Repeat these last four steps for each loop to be changed

13. Click {OK} to return to the Loops tab of the “New Recipe Setup” window.

The Recipe is now sufficiently complete to start a Batch. Many other Recipe functions areavailable, but they are not necessary to start a Batch. They will be covered in later

sections ( see Section 5.2 for a cross-reference guide of the most popular features).

Before you start a Batch, however, you need to save the Recipe:

1. Click {Save}. The “BioCommand Save Files” window will open.

2. Enter a file name in the File edit box, identifying the drive, folder, name, andextension. Click on the down arrow for sample file names (note that the File extension

must be .RCP and that your default recipe folder is BCPLUS). A typical file name

might be C:\BCPLUS\Recipe1.RCP.


54/205

40


3. Click {OK}. The “New Recipe Setup” screen re-appears.

4. Click the {Save} button. At this point you can either close the Recipe window or start

a Batch from this recipe.

5. If you opt to close, the “Save Changes” window will open. Click on {Yes}.

5.4 Starting A Batch

Before starting a Batch, prepare the fermentor. Be certain that these steps have been

taken:

• The fermentor and controller are powered on.

• There is media in the vessel.

• All appropriate liquid and/or gas addition systems are attached to vessel.

• The vessel, media and liquid addition systems have been sterilized as required.• The probes have been calibrated.

• All ancillary equipment has been connected, powered on and tested.

Any other procedures that need to be performed prior to starting a culture should becompleted before starting computer supervision and control.

Most users prefer to start the Batch in BioCommand Plus before inoculating. Doing so

sufficiently in advance establishes correct growth conditions according to the Recipe, andenables confirmation of data logging and other functions before growth begins. The

Elapsed Fermentation Time clock can be reset to zero when inoculation or any other

biologically significant event occurs.

5.4.1 Selecting a Recipe

To start a Batch, you first need a Recipe. There are several ways to select the

desired recipe:

(1) “Starting BioCommand” Screen

A. Click on Open an existing recipe (see screen below):


55/205

41


B. Click on {Continue}. The “Select File to Open” screen will appear (see

sample screen below).

C. Click on the recipe of interest.

D. Click on {Open}. The “Select File to Open” screen will disappear, and

your selected recipe will be displayed in the “View Recipe Setup” screen.

You will be able to verify the recipe parameters by cycling through thetabs. If you wish to modify the recipe, and you have the appropriate

authorization level, see Section 12.1 for more information.

E. Continue in Section 5.4.2 below.


56/205

42


(2) Main Screen File Menu – New Batch

Click on File, then New Batch from the dropdown menu. The “Select File to

Open” screen will open (see sample above), with the “New Recipe Setup” screen

shadowed behind it.

(3) Main Screen Toolbar – New Batch Icon

Click on the New Batch Icon. The “Select File to Open” screen will appear (seesample above), with the “New Recipe Setup” screen shadowed behind it.

(4) “Starting BioCommand” Screen to Extract Recipe

This option is handy if you know the Batch File Name but not the Recipe File

Name.

A. Click on Extract a recipe from a batch and then click on {Continue}. The“Select File to Open” screen will appear, with the “View Recipe Setup”

screen shadowed behind it. Note that this “Select File to Open” screen will

show all available Batch files (*.BDB), instead of recipe files (*.RCP). See

sample screen below:


57/205

43


B. Select the Batch file of interest, then click on {Open}.C. The “View Recipe Setup” screen will open, displaying the recipe (and its parameters) from the selected batch.

D. Verify the parameters by cycling through the tabs of this screen.

Now that you have selected a Recipe, if you wish to modify parameters for the new

batch, and if you have the appropriate authority level, you may do so at this time. See

Section 12.1 for more information. Note that changing parameters for a Batch doesnot alter the Recipe file.

5.4.2 Starting the Batch

1. When you are satisfied with the parameters and ready to start the Batch, click the{Start Batch} button. The “Batch Display Name” screen appears:

2. Choose a Batch display name and click OK.

3. The “BioCommand Save Files” window will open:


58/205

44


4. To see existing File Name/Folder strings, click on the down arrow () to the rightof the ‘File’ edit box.

5. Click on an existing File Name/Folder string to bring it into the edit box as a pattern. Type over it to create a unique name for the Batch datafile. This will not

affect the existing File Name in any way.

6. If this file should be available to your network, click in the check box next toShared over the network .

NOTE:All batch file names must have the extension “.BDB”.

A batch tab with the suffix letter “B” and the previously selected Batch Display Name

appears at the bottom of the BioCommand Plus screen. Use this tab to select which

Batch to view when several are running.

The batch is now running, as indicated by the ‘Current EFT’ (Elapsed FermentationTime) clock running in the Setup screen.

5.5 Batch Summary View

You can view a text summary of current Batch status by calling up the Batch Summary

View. BioCommand Plus enables many other displays, including synoptic views, trend

graphs, bar graphs, etc., but these must first be specified on the Views page of the Recipeor Batch setup screens before they are available for use in the batch. (See Sections 6 and

7 for information on other views.)

The Batch Summary view is available by default in all recipes.

There are several ways to display the Batch Summary View:

(1) From the Setup window, click on the Batch name tab at the bottom of the

BioCommand Plus screen.


59/205

45


(2) From the Main Menu, select View – Batch Summary.

(3) From the Setup window, click on the Views tab, then double click on Batch

Summary in the ‘Current Views’ pane.

The Batch Summary view will open (see sample screen below):

Place your cursor on any edge of the window. When the double-headed arrow (↔)appears, drag the edge to open the screen more fully.

Batch Summary View Column HeadingsColumn Heading Description

Loop Loop Name

CV Current Value of loop

Units Measuring Units for Current Value

SP Loop Setpoint

OU Controller Output for loop

Mode Control Mode for loop


60/205

46




61/205

47


66 IINNTTRROODDUUCCTTIIOONN TTOO BBAATTCCHH VVIIEEWWSS

In BioCommand Plus , trend graphs, bar gauges and the Batch Summary displaycombine with BioCommand Plus synoptic display elements to form a virtually

limitless number of possible process viewing screens. Master the easyBioCommand Plus display tools, and you can create informative, convenient,

appropriate displays, customized to your hardware and process.

6.1 Trend Views

A Trend View is a line graph of fermentation data plotted against time. You may

select up to 8 different data items to plot in real time while the Batch is running.Each data item is displayed in a unique color so that the plots may be easily

distinguished from each other. A different vertical scale may be assigned to each

plot.

A Trend View can be specified in the Recipe Setup window, or added to the Batch

view after the Batch is running. To create a simple Trend View:

1. From the main menu, select File-View Recipe, or click on the View Recipe icon.

2. Select the Recipe of choice.

3. From the recipe Setup window, select the Views tab (see sample screen below):


62/205

48


4. Double-click on Trend in the ‘New Views:’ pane to open the Trend window,

which will look like the sample screen below:

5. Select the “Loops” tab in the control panel at the bottom of the window. Thischanges the control panel to the ControlLoop selection screen, as illustrated

below:

6. Click on the {Setup Loop1} button to open the “Select Loop” list. These are theavailable loops that have been specified for this recipe:


63/205

49


7. Click on the loop that is desired for the first plot and then click the {OK} button.

This will add the loop name to the ‘ControlLoop’ window. Click on the {>>} button to change the control panel to Setup Loop2. This procedure may be

repeated for as many loops as you want to plot, up to a total of 8.

8. Select the “Graphs” tab on the control panel for the Trend View. The control panel will change to a screen as shown below.

This allows you to set the high and low limits on the y-axis for each data item that is

to be plotted. Normal Windows

editing techniques will allow any of the numerical

parameters to be changed.

You may also edit the Start and End times for the Trend View. Note, however, that

changing either time value will change the value for all parameters plotted.

9. Click on the {>>} and {


64/205

50


13. Close the window to add this view to the Recipe. It will appear in the

‘Current Views’ pane the next time you return to the “Views” tab.

NOTE: If the trend graph was entered as part of the New Recipe or View Recipefunction, the trend graph will be available to all batches initiated fromthe recipe.

If it was entered from a Batch function, then the trend graph will beavailable only in the present Batch.

6.2 Using the Trend Graph

When the Trend View is onscreen, the “Main” tab should be selected on the control

panel. This will present a screen on the control panel with 8 display windows on it.Each window, as illustrated in the sample screen below, will display a loop name in

the color of the plot, as long as the cursor is outside the plot area.

If the cursor is moved inside the plot area (i.e., onto the graph), each of thesewindows will display the current value for the loop, as illustrated below:

If the Batch is not running, these values will all be 0 (as indicated in the samplescreen above).


65/205

51


When the view is first entered, the left y-axis will display the name, color and scale

for Loop1, and the right y-axis will be named, colored and scaled for Loop2. In theabove sample, Loop1 (Agit1) is on the left, and Loop2 (pH1) is on the right.

Clicking on the loop name on the control panel “Main” tab will shift these labels.

With each click the new loop label will replace the current label, first on the left side,then the right side. It will alternate with each succeeding click.

You can change the Trend View to show a larger graph area by clicking anywhere onthe gray areas of the view. The control panel will reduce to colored buttons, each

representing its assigned loop. Click again in the gray area to return to the tabbed

control panel.

There are many other features available in Trend Views. See Chapter 13, Creating

and Editing Views, for details.

6.3 Creating Synoptic Views

Synoptic Views pictorially represent the physical layout of a fermentation system.

Create a synoptic view by using a toolbar to position icons of fermentation systemcomponents in the display space. Beside these icons, you can place data values and

labels that describe the system and the status of the Batch.

To create a synoptic view:

1. From the main menu, select File-View Recipe, or click on the View Recipe icon.

Select a recipe you created previously.

NOTE: You can also create synoptic views while a Batch is running, from theSetup screen.

2. Select the Views tab. This page shows the currently defined views in the left

pane. It shows views that can be defined in the right pane.

3. Double-clicking on Synoptic in the ‘New Views:’ pane opens the Synopticwindow.

4. A Toolbar appears at the right side of the screen when this window opens and anew item, Synoptic, is added to the Main Screen menu items, as shown below:


66/205

52


5. Select Synoptic – Design Mode from this menu. A check mark appears beside

Design Mode in the menu and a grid of dots appears on the Synoptic designscreen:

6. Select the vessel icon from the Toolbar (see Section 13.3 for details on theSynoptic View Toolbar) or select Synoptic – Gauges – Vessel from the Main

Menu.

7. When you move the cursor onto the synoptic screen, a rectangle will be attachedto the cursor arrow.

8. Move this rectangle to the desired location on the screen and click the left mouse button to anchor the vessel icon (see sample screen below with vessel icon).


67/205

53


As illustrated above, the default label “Vessel” appears with this icon. To change it

to an NBS fermentor name:

A. Double-click on the label (“Vessel”).

B. Click on the down arrow (

) that appears, to pull down a list of the standard NBS Fermentation System names (see sample below).

C. Select the desired name by clicking on it.

D. To enter a custom label, type in the new name and press .

E. If necessary, you can right-click on this label to use the Delete function.

F. To enable Agitation via this Synoptic View, right click on the Vessel icon to

access the pop-up menu. Select Properties, then select Agit from the Loop

Select Window, then click {OK}. “Agit = X” (“X” is the current value) willappear in the Vessel icon edit box next to the image of the impellers.

A Faceplate is a bar graph display of the Setpoint (‘SP’), Current Value (‘CV’), and

Output Level (‘OU’) for any loop in the recipe.

9. Select the faceplate icon on the Toolbar (see Section 13.3 for details on the

Synoptic View Toolbar) or select Synoptic – Gauges – Faceplate from the MainMenu.

Moving the cursor onto the synoptic screen will show a rectangle attached to thecursor arrow.

10. Move the rectangle to the desired location on the screen.

11. Click the left mouse button to place the faceplate icon there.

12. Click on the bar at the bottom of the faceplate with the right mouse button to opena submenu.


68/205

54


13. Click on Properties.

14. The “Select Loop” window will open, showing a list of the loops available for this

Recipe.

15. Click on the desired loop name.

16. Click on the {OK} button.

17. The bottom bar will turn red, and will also display the name of the loop you

chose:

The above sample shows no active information because no batch is running.

When a Batch is running, the Setpoint (SP), Current Value (CV), and Output Level

(OU) will be displayed as bar graphs. The top of the display will show the actualvalue for the loop setpoint, current value, or output level--whichever option button is

selected just below it. It will also identify the control mode for the loop.

The submenu that appears when you right click the loop label bar allows you tochange the setpoint: select Setpoint Change to open the “Change Setpoint / Control

Mode” window.

You can click on the scale end values for each parameter to change the bar graph

range to values more appropriate to the expected data range. The figure below shows

the Setpoint and Current Value ranges changed for an agitation speed loop:


69/205

55


The Setpoint and the Current Value scales were changed from 0-100 to 0-1000.

Now add a Flowmeter to the display:

18. Select a Flowmeter from either the menu or the Toolbar (see Section 13.3 for

details on the Synoptic View Toolbar).

19. Place it at an appropriate location on the Synoptic screen; click to anchor it.

20. Right-click on this icon to open a submenu.

21. Select Properties.

22. The “Select Loop” window will open.

23. Select the loop that this icon will represent. The name and current value of the

selected loop is displayed above the icon:

Now draw a line to connect the flowmeter to the vessel in this simple synoptic

display:

1. Select Synoptic – Lines – Draw Lines from the Main Menu, or click on the Draw

Lines icon () on the Toolbar.

2. Move the cursor onto Synoptic View screen: it will change to a + shape.

3. Place the cursor at the inside edge of the flowmeter, then click the left mouse

button.

4. Move the cursor toward the vessel icon. As you draw the line, it will be dashed,

not solid, until you anchor it.

5. You may only draw straight lines. If you need to change directions, click the left

mouse button at the end of the line (the dashed line will change to a solid line),then continue drawing in the new direction. Note that you may only make rightangles.

6. Double-click to anchor the end of the connecting line.

7. Double-click on a blank area of the screen to end the Draw Lines function.


70/205

56


NOTE:A line may be drawn only between an output and an input (or viceversa), not between two outputs or two inputs.

Change the Synoptic View to Run Mode:

1. Click on the Design Mode icon on the toolbar or select Synoptic – Design Mode

from the Main Menu.

2. Close the Synoptic View window.

3. The Recipe Setup window will list a new Synoptic view.

The above procedure has created a very simple Synoptic View for the Batch being run,as shown below:

Note that in this sample, the Faceplate gauge identifies the Agitation loop’s CurrentValue (selected by option button) as 600 and the Control Mode as PID at the top of

the icon. It also displays current values for all three parameters in the bar graphs.The Flowmeter, which is connected to the Vessel by a short line, identifies Air Flowat a Current Value of 40. The animation feature of this view will show the impellers

turning in the vessel.

For further information on the features available in Synoptic Views, see Chapter 13

on Creating and Editing Views.


71/205

57


77 CCRREEAATTIINNGG PPRROOGGRRAAMMSS

BioComman Plus has powerful programming tools that can alter setpointsautomatically at specified times, or when specified culture conditions are met.

Programs are useful for implementing feeding strategies, inducing protein

production through temperature shifts or chemical additions, and generallyrelieving the operator of having to be present in order to carry out a protocol.

Programs can also calculate values that are not directly measured by probes.

For example, from agitation RPM, the linear speed of the impeller tip can be

calculated, logged, displayed and otherwise utilized in the same ways as adirectly measured loop value.

Programming tools include:

• Time Profile Functions, which change setpoints as prescribed by the user according to

elapsed fermentation time

• Logical and mathematical “function blocks”, which are graphic elements with uniquefunctions such as add, assign a setpoint, compare values, and calculate a value, among others.

• User-Defined Function blocks, programmed by the user in the BASIC programm

guia de operacion biocomand

Documents