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doi:10.1016/S0211-139X(05)74883-6

Revista Espaola de Geriatra y

Gerontologa

Volume 40, Issue 6, December 2005, Pages 351356

Originales

El dao peroxidativo en leucocitos peritoneales de ratonesviejos disminuye si se suplementa la dieta con galletasenriquecidas con antioxidantes. Relacin con la supervivencia

Lipid peroxidation in peritoneal leukocytes from aged mice is

decreased by antioxidant dietary supplementation. Relationship with

survival

C. Alvarado-Fraduaa, P. lvarez-Cifuentesa, N. Guayerbas-Valeroa, M. Puerto-Canteroa,L. Jimnezb, M. de la Fuente del Reya, ,

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Resumen

Introduccin

el envejecimiento est asociado a un estrs oxidativo crnico que se manifiesta en un

mayor dao a macromolculas como es el caso de los lpidos, y el producto de

degradacin ms representativo de stos es el malondialdehdo (MDA). Los leucocitos

producen compuestos oxidantes para realizar sus funciones y son muy sensibles al dao

oxidativo. Por ello, se han analizado las concentraciones de MDA en leucocitos

peritoneales de ratones durante el envejecimiento, y el efecto, en animales viejos, de unadieta enriquecida con antioxidantes en estas concentraciones, as como su posible

incidencia en la longevidad.

Material y mtodos

el MDA se valor por HPLC (highperformance liquid chromatography) en leucocitos

peritoneales de ratones de la cepa ICR (CD 1) jvenes (16 2 semanas), adultos (36 2

semanas), maduros (52 2 semanas) y viejos (71 2 y 81 2 semanas), y en los de

estos ltimos tras la ingestin, durante 5 y 15 semanas de una dieta suplementada (en

un 20% p/p) con galletas enriquecidas en antioxidantes. Estos animales viejos ingirieron

hasta su muerte la dieta suplementada, para obtener la posible relacin entre la ingestin

de esta dieta y la longevidad.

Resultados

los valores de MDA aumentan con la edad, y la ingestin, en animales viejos, de una

dieta enriquecida con antioxidantes, tanto durante 5 como 15 semanas, los disminuye;

se observa una relacin inversa entre los valores de MDA y la longevidad.

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Conclusiones

en animales viejos, la ingestin de una dieta enriquecida en antioxidantes disminuye el

grado de peroxidacin lipdica en leucocitos peritoneales, lo que se refleja en un

aumento de su longevidad.

Abstract

Introduction

ageing is associated with chronic oxidative stress, which leads to increased damage to

macromolecules, such as lipids. One of the most important end products of lipid

peroxidation is the malondialdehyde molecule (MDA). Leukocytes produce reactive

oxygen species to support their functions and are highly sensitive to oxidative damage.

The aim of this study was to ascertain MDA levels in peritoneal leucocytes from mice

during ageing, as well as the effect of antioxidant dietary supplementation in aged mice

on the levels of this molecule and its possible incidence on longevity.

Material and methods

MDA levels were assessed by highperformance liquid chromatography in peritoneal

leukocytes from ICR (CD 1) mice at young (16 2 weeks), adult (36 2 weeks), mature

(52 2 weeks) and old (71 2 and 81 2 weeks) ages. At the latter age, MDA contentwas also measured after 5 and 15 weeks of dietary supplementation (20% w/w) with

biscuits enriched with nutritional doses of antioxidants. The mice received the

supplementation until death to ascertain the possible relationship between ingestion of an

antioxidant-enriched diet and longevity.

Results

MDA levels increased during ageing, whereas the levels of lipid peroxidation decreased

after 5 and 15 weeks of antioxidant dietary supplementation in aged mice. Moreover, an

inverse relationship between MDA content and longevity was observed.

Conclusionsour results demonstrate that in aged mice ingestion of an antioxidant-enriched diet

decreases lipid peroxidation in peritoneal leukocytes, leading to increased longevity.

Palabras clave

Envejecimiento; Malondialdehdo (MDA); Leucocitos; Antioxidantes; Dieta; Peroxidacin

lipdica; Estrs oxidativo

Key words

Ageing; Malondialdehyde (MDA); Leukocytes; Antioxidants; Diet; Lipid peroxidation;

Oxidative stress

Este trabajo ha sido financiado por Danone Vitapole (Francia). Este trabajo ha

recibido el premio Paella Casas 2004 a la major comunicacin oral del rea

Biolgica presentada en el XLVI Congreso nacional de la Sociedad Espaola de

Geriatra y Gerontologa, celebrado en Las Palmas de Gran Canaria del 9 al 12 de

junio de 2004.

Correspondencia: M. de la Fuente. Departamento de Fisiologa. Fisiologa Animal II. Facultad deCiencias Biolgicas. Universidad Complutense de Madrid. 28040 Madrid. Espaa.

Copyright 2005 Sociedad Espaola de Geriatra y Gerontologa. Published by Elsevier Espaa S.L. Allrights reserved.

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