universidad autónoma metropolitana unidad xochimilco. · pichilingue yavaros 214.20 199.20 387.5...
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Project: Artemia biodiversityProject: Artemia biodiversity
Universidad Autónoma MetropolitanaUnidad Xochimilco.
Calzada del Hueso No. 1100. Colonia Villa Quietud. México, 04960, D.F.
Calzada del Hueso No. 1100. Colonia Villa Quietud. México, 04960, D.F.
Departamento El Hombre y su Ambiente. Laboratorio: Producción de Alimento Vivo para la Acuicultura.
Departamento El Hombre y su Ambiente. Laboratorio: Producción de Alimento Vivo para la Acuicultura.
TelTel. (52) 5483 7151. (52) 5483 7151Fax: (Fax: (5252) 5483 7469) 5483 7469
Project: Artemia biodiversityProject: Artemia biodiversity
Participants of the project and field study: Participants of the project and field study:
PhD. Thalía Castro Barrera [email protected] of the Project. Ecological studies ofnatural populations.
Biol. Jorge Castro Mejía [email protected], reproduction and isolation studieswith Artemia, Artemia culture in natural habitats. Cladoceran, Copepods and Tenebrios culture.
Project: Artemia biodiversityProject: Artemia biodiversity
Participants of the project and field study: Participants of the project and field study:
Biol. Germán Castro Mejía [email protected] of new populations. Artemia culture in natural habitats. Microalgal, Cladoceran, Copepodsand Tenebrios culture.
Biol. Aida Malpica Sánchez [email protected] of total lipids. Fatty acids and Aminoacidsanalisys.
Project: Artemia biodiversityProject: Artemia biodiversity
Participants of the project and field study: Participants of the project and field study:
Biol. Ramón de Lara Andrade [email protected] field studies with Artemia andPanagrellus culture.
Ag. Ing. Benjamín Miramontes [email protected] Soil and water ion andcation analisys
Project: Artemia biodiversityProject: Artemia biodiversity
Participants of the project and field study: Participants of the project and field study:
Ph.D. Horacio Sandoval [email protected] Responsible of theBiochemical analisys.
Ph.D. Francisco Correa Sandoval (UABC). [email protected] Responsible of geneticstudies with Artemia.
Project: Artemia biodiversityProject: Artemia biodiversity
Project: Artemia biodiversityProject: Artemia biodiversity
Ionic water composition of natural Artemia habitats.
Ionic water composition of natural Artemia habitats.
Name Cl (Mg/l) SO4 (mg/l) HC03 (mg/l) CO3 (mg/l) Na (mg/l) K
(mg/l) Ca (mg/l) Mg (mg/l)
Cuatro Cienegas de Carranza 28 930 61 440 9 150 24 790 3 130 0 13 280
Bahía de Ceuta 77 790 14 164 5 760
Salinas de Hidalgo 2 240 228 52 84 2 696 230
Real de las Salinas 15 120 100 700 0 13 500 1 350 0 100
Texcoco 1 438 26 610 48 15 590 20 322
Project: Artemiabiodiversity
Project: Artemiabiodiversity
LagoonPermanentThalasohalineSistema lagunar de: Laguna del mar Muerto
SalinePermanentAthalasohalineLas Coloradas
SalinePermanentAthalasohalineTexcoco
Lagoon, salinePermanentThalasohalineCelestúm
Lagoon, salinePermanentThalasohalineReal de las Salinas
SalinePermanentThalasohalineSan Crisanto
SalinePermanentAthalasohalineSalinas de Hidalgo
Lagoon, salinePermanentThalasohalineEl Barranco (Altamira)
Lagoon, salinePermanentThalasohalineBahía de Ceuta
SalineTemporalThalasohalinePichilingue
LagoonPermanentThalasohalineBahía de Ohuira
Lagoon, salinePermanentThalasohalineSalina Tres Hermanos
SalinePermanentThalasohalineIsla del Carmen
SalinePermanentThalasohalineGuerrero Negro
PuddleTemporalAthalasohalineCuatro Cienegas de Carranza
PuddleTemporalAthalasohalineSan Jose
SalinePermanentThalasohalineLa Salina
BiotopeSeasonalOriginName
Project: Artemia biodiversityProject: Artemia biodiversity
Guerrero Negro
401.0203.00221.00Bahia de ohuira
423.7231.90240.60Las Salinas
479.7221.00230.80San Crisanto
Pichilingue
387.5199.20214.20Yavaros
211.80228.70El Barranco
411.5209.20224.80San José
432.0225.00240.00Las Coloradas
460.0224.50245.50Celestún
479.7221.00230.80Cuatro Cienegas De Carranza
Sistema Lagunario Mar Muerto
379.7203.00215.90Bahía de Ceuta
479.0224.80234.80Texcoco
411.0218.01241.78Real de las Salinas
Nauplii sizeDecapsulated cystdiameterFull cyst diameterName
Project: Artemia biodiversityProject: Artemia biodiversity
XGuerrero Negro
XXXXBahía de Ohuira
XXXXLas Salinas
XXXXXSan Crisanto
Pichilingue
XXXXXXYavaros
XXEl Barranco
XXXSan José
XXXXLas Coloradas
XXXXCelestún
XXXXCuatro Cienegas
Sistema Lagunario Mar Muerto
XXXXXBahía de Ceuta
XXXXXTexcoco
XXReal de las salinas
NaupliiCysts
InterbreedingCromosomesElectrophoresisMorphometryType of reproductionName
Project: Artemia biodiversity
Analytic methods Analytic methods
Protein determination.
The method applied is macro-Kjedhal which is recommended by A.O.A.C. (1997), using 9.0 g dry weight of cysts, nauplii or adults.
Determination of total carbohydrates.
Dubois (1956) technique is used. Samples of 1.0 mg dry weight ofcysts, nauplii and adults, were introduced in tubes of glass of 3.5 x 200 mm, and added 1 ml of phenol (5%) and 5 ml of sulfuric acid. The concentration of total carbohydrates was determined with photocolorimeter Spectronic 20D at 500 nm. The solution pattern used was sucrose.
Project: Artemia biodiversityProject: Artemia biodiversity
Analytic methods Analytic methods
Total lipids analysis. Total lipids analysis.
They were determined by the Technique of Soxhlet(Rubinson and Rubinson, 1998), using 100 mg dry weight of cysts, nauplii or adults.
They were determined by the Technique of Soxhlet(Rubinson and Rubinson, 1998), using 100 mg dry weight of cysts, nauplii or adults.
The samples were washed with 20 ml of hexane, chloroform and acetone during 2 hours. After, the samples were placed in a dry container with silica gel during 10 hours. Then were weight and applied an equation to determined the percentage of total lipid.
% of total lipids = Weigh of the final sample X 100/initial weight
The samples were washed with 20 ml of hexane, chloroform and acetone during 2 hours. After, the samples were placed in a dry container with silica gel during 10 hours. Then were weight and applied an equation to determined the percentage of total lipid.
% of total lipids = Weigh of the final sample X 100/initial weight
Project: Artemia biodiversityProject: Artemia biodiversity
Analytic methods Analytic methods
Determination of aminoacids
The liquid chromatography of high pressure was used with a cation exchanged resin (Beckman, 1985), using 2.0 g dry weight of cysts, nauplii or adults of each sample. An automatic analyzer (Beckman System Gold 6300) was used.
Determination of aminoacids
The liquid chromatography of high pressure was used with a cation exchanged resin (Beckman, 1985), using 2.0 g dry weight of cysts, nauplii or adults of each sample. An automatic analyzer (Beckman System Gold 6300) was used.
Determination of essential fatty acids.
The lipids were extracted by the Technique of Soxhlet and then the esterfication of the fatty acids were with sulfuric acid (to 6% in methanol), at temperature of 85°C. After 1.5 ml of distilled water and 2.0 ml of hexane were added. Two layers are formed and from the upper layer 1 microliter is extracted and injected in a gas chromatography (Perkin Elmer Autosystem 9000), with a polar column (Restek Stabilwax30mX0.53mmX0.50 m), with polietilenglicol phase.
Determination of essential fatty acids.
The lipids were extracted by the Technique of Soxhlet and then the esterfication of the fatty acids were with sulfuric acid (to 6% in methanol), at temperature of 85°C. After 1.5 ml of distilled water and 2.0 ml of hexane were added. Two layers are formed and from the upper layer 1 microliter is extracted and injected in a gas chromatography (Perkin Elmer Autosystem 9000), with a polar column (Restek Stabilwax30mX0.53mmX0.50 m), with polietilenglicol phase.
Project: Artemia biodiversityProject: Artemia biodiversity
Determination of ionic composition of soil and waterDetermination of ionic composition of soil and water
Spectrophotometry (Atomic Absorption Spectrophotometer SpetrAA Variant). To determine: Ca, Mg, Fe, Mn, Cu, Zn, Pb, Cd, Al.
Spectrophotometry (Atomic Absorption Spectrophotometer SpetrAA Variant). To determine: Ca, Mg, Fe, Mn, Cu, Zn, Pb, Cd, Al.
UV Spectrophotometer (Shimatzu 205), to determined: NH4
+, NO3, NO2 and Phosphate. UV Spectrophotometer (Shimatzu 205), to determined: NH4
+, NO3, NO2 and Phosphate.