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8/3/2019 Presentation Como

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Dyes in textile effluents -

role of dyeing (printing) technologies and dye structures

Biotreatment of dyes in effluents

classical aerobic sewage treatment process adsorption on the sludge that

depends on the dye solubility

acid dyes their high solubility leads to low adsorption ( depending on the degree of sulfonation)

direct dyes high level of adsorption not depending on sulfonation

reactive dyes very low degree of adsorption

basic dyes high adsorption

disperse dyes adsorption in the high to medium range

Reactive dyes

high consumption, applied in high concentrations in some technologies

relatively high concentrations in textile effluents

small molecules, high water solubility

decolourization

dearomatization


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Pathways for the aerobic

microbial degradation of man-

made sulfonated azo dyes by

peroxidases from

Phanaerochete chrisosporium

Primarily asymmetric

cleavage of azo linkage is

supposed that leads to

obtaining of sulfonamide

and phenolic structures


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Advantages of covalently immobilized enzyme systems

immobilized whole cells keep a high level concentration

of intracellular inducible multi-enzyme system

activity of these systems remains relatively high as far as

the immobilization does not affect the cell integrity

possibility for use in reactors for continuous processes


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IMMOBILIZATION of the FUNGI STRAIN of

Trichosporon cutaneum and Phanaerochete chrisosporium

carrier activated acrylamide copolymer

treatment of granules with cell suspension

suspending in synthetic nutrient medium

( 24 hours treatment)

washing

control for free cells ( spectrophotometrically)

microscopy


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Table 1Microbial strainMicrobial strain CarrierCarrier ImmobilizedImmobilized

cellscells ((mg/g drymg/g dry

carrier)carrier)

TrichosporonTrichosporon

cutaneumcutaneum

SyntheticSynthetic polymerpolymer

granulesgranules

38.4/38.4/8,48,4forforCellulose granulesCellulose granules

PhanerochetePhanerochete

chrysosporiumchrysosporium

SyntheticSynthetic polymerpolymer

granulesgranules

42,342,3


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PHENOL TRANSFORMATIONABILITY

OF IMMOBILIZED CELLS

adaptation in a phenol medium (0,1g/l)

phenol transformation at 0,35 g/l , 0,65 g/l

and 1g/l substrate concentration

HPLC measurements of phenol concentration(absorbance at 256 nm)


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KINETICS OF PHENOL CONVERTION

Cells Trichosporon cutaneumTrichosporon cutaneum

0,35 g/l phenol (Immobilized cells

proliferate, remaining stable and

active for 22 cycles)

0,65 g/l phenol

1,00 g/l phenol


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BIODEGRADATION OF PHENOL BY

MULTYENZYME ENZYME PRODUCED IN

IMMOBILZED Trichosporon cutaneum CELLS

Hydroxylation

of phenolic

structures

Dearomatization by

oxidase/hydrolase

system in

Trichosporon

Cutaneum


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STUDYOF Phanarochete chrysosporium BEHAVIOUR

immobilized cells showed a relatively slow phenoldegradation compared to Trichosporon cutaneum

source of exo-oxidase enzyme system containing

lccase, lignin peroxidase and manganese peroxidase

ultrafiltration and immobilization of the aboveenzyme system

characterization of the immobilized system with

respect of manganese peroxidase activity

Michaelis constant of the reaction with Phenol Red

has the same range for free and immobilized

manganese peroxidase


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EnzymeEnzyme BoundBound

proteinprotein

[mg/g[mg/g

drydry

carrier]carrier]

pHpH

optimumoptimum

TemperatureTemperature

optimumoptimum [[00C]C]

SpecificSpecific

activityactivity

[U/mg][U/mg]

RelativeRelative

activityactivity

[%][%]

KKmm

[M][M]

FreeFree

manganesemanganese

peroxidaseperoxidase-- 4,54,5 3030 1,3901,390 -- 6.106.10

--55

ImmobilizedImmobilized

manganesemanganese

peroxidaseperoxidase

6,26,2 4,54,5 3535 0,1110,111 8,028,02 7,8.107,8.10--55

Table 2

Phenol Red


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KINETICS OF BEZAKTIV Brill.RED V-GG DECOLOURIZATION

BYTHE IMMOBILIZED ENZYME SYSTEM PRODUCED OF

Phanaerochete chrisosporiumI step - dye water solution treatment (decolourization)

II step possibility for dearomatization with immobilized Trichosporon

cutaneum cells should be additionally investigated


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CONCLUSIONS

Fungi can be immobilized at high concentration

covalently to synthetic beads retaining a high levelof stability and activity

Immobilized cells are viable and can be used as a

source of multy-enzyme systems

Trichosporon cutaneum immobilized cells can beapplied for phenolic structures dearomatization

Phanaerochete chrisosporium oxidase multy-

enzyme immobilized on synthetic beads decolourize

monoazo dyes in water solution

Trichosporon cutaneum immobilized cells

treatment of decolourized solutions have a potential

to be used for dearomatization of above solutions

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