eurosurfas 2011: jornadas medioambiente - anna surribas
DESCRIPTION
Aplicaciones industriales de la biocatálisisTRANSCRIPT
Aplicaciones industriales de la biocatálisis Nombre conferencia
Aplicaciones industriales de la biocatálisis
Anna Surribas Elena Genescà, Aleix Conesa, Roshan Paul, Marolda Brouta-Agnésa i Meritxell de la Varga.
Barcelona, 16th November 2011
Aplicaciones industriales de la biocatálisis
Aplicaciones industriales de la biocatálisis
LEITAT’s Corporate presentation
3
(*) Images: applications of Leitat Technological Center to the FotCiencia’06, FotCiencia’07, FotCiencia’08 contests:
1.- Tineoella bisselliella larva adhered to a wool fiber
2.- Surface tension modified by plasma treatment in a textile surface
3- Poliamides (transversal section)
4- Scratch test of ABS (Acrilonitrilo, Butadieno, Estireno)
OBJECTIVE
R+D+2I collaboration, adding technological value and a clear adaptation and evolution to the constant market changes.
Aplicaciones industriales de la biocatálisis
4
R+D+i market oriented services
Aplicaciones industriales de la biocatálisis
5
Industrial Biotech:
R & D in biotechnological solutions with application in industrial processes; applied
microbiology and microencapsulation.
Biocatalysis
Industrial Bioprocesses
Bioproduct production
Environmental Biotechnology
Applied microbiology (process validation, biofilm models, strain isolation and characterization…)
Microbiology testing
Microencapsulation of antimicrobial products
Aplicaciones industriales de la biocatálisis
Aplicaciones industriales de la biocatálisis
7
–
–
–
Aplicaciones industriales de la biocatálisis
Fibres separation process
Cultivation
Water retting
Air drying
Oven drying
Grinding (siglo XVIII)
8
Aplicaciones industriales de la biocatálisis
Project substrates
9
Aplicaciones industriales de la biocatálisis
Enzymatic retting set up
10
Commercial enzymes Proveedor
Texazym BFE (pectinase) Inotex
Polygalacturonase Biocon
Xylanase Biocon
Scourzyme (pectinase) Color Center
Process variables
Enzyme cocktail
Process time
Temperature
pH
Buffer / Chelant agent
Agitation type
Fiber polishing
Bath/Fiber ratio
Aplicaciones industriales de la biocatálisis
Results of the project
11
Fibers separation with enzymes (P6):
– Ecological separation process.
– Low temperature process.
– Short time process (catalyzing action of the enzyme).
Fibers obtained with the enzymatic process (P6).
Aplicaciones industriales de la biocatálisis
Results of the project
12
•
•
•
•
•
Mechanically
treated and
enzymatic
polishing of fibers.
Mechanically
treated fibers.
Hemp skin fibers polished with
enzymes.
Aplicaciones industriales de la biocatálisis
Aplicaciones industriales de la biocatálisis
14
Substitution of conventional cotton wet processing :
(1) less harmful
(2) less aggressive towards the environment and fibre
(3) applied at mild conditions
(4) cost-effective
Aplicaciones industriales de la biocatálisis
15
Process 2: chemical processing
Aplicaciones industriales de la biocatálisis
16
- Enzymatic desizing and scouring in shorter
time.
- An alternative amylase and pululanase
(Dextrozyme) for the desizing/scouring. A
pectinase, humectant, chelating agent and buffer
KH2PO4 are used.
- Process conditions : 62ºC and 60 min at pH 4.1
- Transfer to a second bath for chemical
bleaching.
Process 2: Two step enzymatic process
Aplicaciones industriales de la biocatálisis
17
One step continuous process
- Enzymatic desizing /scouring with a
commercial thermostable amylase (Texazyme
TA10)
- Simultaneous chemical bleaching with H2O2
- Process conditions : 105ºC during 60 min.
Single step continuous enzymatic process using thermoresistent enzymes
Process 2: One step preindustrial process
Aplicaciones industriales de la biocatálisis
SEM Images
18
A B C
Scanning Electron Microscopy pictures of treated cotton fibres.
(A)Chemical desizing, scouring and bleaching
(B)Two step enzymatic process
(C) One step continuous enzymatic processing
Aplicaciones industriales de la biocatálisis
Textile Quality
19
Weight loss Whiteness
(CIE) Iodine test Drop test
Conventional Treatment 15% 63,1 OK OK
Enzymatic semicontinuous treatment
11,75% 65 OK OK
Alternative enzymatic semicontinuous treatment
13,37% 61 OK OK
Single step enzymatic process
12,57% 71 OK OK
QUALITY ASSESSMENT:
- Desizing and scouring : iodine test and the water penetration test (UNE EN 20811).
- Bleaching: CIE whiteness using DATACOLOR spectrophotometer.
- Weight loss and tensile properties of the fabrics (EN ISO 13934/1:1999)
Aplicaciones industriales de la biocatálisis
Reutilizations and Savings
20
Aplicaciones industriales de la biocatálisis
21
Industrialization and Tech Transfer
and finishing of
home textiles, technical textiles for filtration and
medical textiles.
Product Laboratory trials (CIE whiteness) Autoclave (CIE whiteness)
Texazyme TA10L 72,9 58,2
Bialfa T 72,8 61,4
Aquazym SDL 69,3 57,1
Aplicaciones industriales de la biocatálisis
Aplicaciones industriales de la biocatálisis
23
Aplicaciones industriales de la biocatálisis
24
0
5
10
15
20
25
30
35
15 20 25 30 35 40 45
SFC
(%
)
Temperature (ºC)
Aplicaciones industriales de la biocatálisis
25
ENZYMATIC
INTERESTERIFICATION
Aplicaciones industriales de la biocatálisis
26
Enzyme Characteristics
E1 Commercial Immobilized non-specific enzyme
E2 Commercial immobilized specific enzyme
Variables
Enzyme type and quantity
Process time
Temperature
Fat /oil ratio
Agitation
Aplicaciones industriales de la biocatálisis
27
0
5
10
15
20
25
30
35
40
15 20 25 30 35 40 45
SFC
(%
)
Temperatura (ºC)
40:60 Fat:Oil E1
40:60 Fat:Oil E2
50:50 Fat:Oil E1
50:50 Fat:Oil E2
60:40 Fat:Oil E1
60:40 Fat:Oil E2
70:30 Fat:Oil E1
70:30 Fat:Oil E2
80:20 Fat:Oil E1
80:20 Fat:Oil E2
Desired SFC
Aplicaciones industriales de la biocatálisis
28
SMP (ºC) 30,7
-0,6
-0,5
-0,4
-0,3
-0,2
-0,1
0
0,1
-60 -40 -20 0 20 40 60 80 100
Hea
t fl
ow
(W
/g)
Temperature (ºC)
Cooling thermogram
0,15
0,2
0,25
0,3
0,35
0,4
0,45
-40 -20 0 20 40 60 80
Hea
t fl
ow
(W
/g)
Temperature (ºC)
Heating thermogram
Aplicaciones industriales de la biocatálisis
Aplicaciones industriales de la biocatálisis
Conclusions
30
Aplicaciones industriales de la biocatálisis
Acknowledgments
31
Aplicaciones industriales de la biocatálisis
Thank you for your attention