actividad de laboratorio 8: "identificación de proteínas"

8/17/2019 Actividad de laboratorio 8: "Identificación de Proteínas"

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C A M P O S B A R O C I O B L A N C AG A R C I A LO P E Z K A R L A

B A S T I D A V A S Q U E Z E L I S AR E V U E L T A M A R T I N E Z J O N A T H A

ACTIVIDAD DE LABORATORIO 8IDENTIFICACIÓN DE PROTEINAS


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HIPOTESIS Y OBJETIVO

• Determ!"r #" $re%e!&" 'e $r(te)!"% e! '%t!t(%"#me!t(% me'"!te #" Re"&&*! 'e B+ret,

• De%"m"(% "$re&"r #" $re%e!&" 'e #"% $r(te)!"%

e! !+e%tr"% m+e%tr"% &(! #" "-+'" 'e +!'e!t.&"'(r,


3/12

INTRODUCCION

• L"% $r(te)!"% %(! e#eme!t(% /t"#e% $"r" #(%(r0"!%m(%1 e!&(!tr2!'(%e e! $#"!t"% - "!m"#e% e!+!" $r($(r&*! e#e/"'", H"- +!" 0r"! /"re'"' 'e$r(te)!"% - &"'" +!" 'e%em$e3" +!" 4+!&*!5(#*0&" e%$e&).&" 6+e $+e'e %er 'e re%er/"1 'e%(%t7!1 tr"!%$(rte1 e%tr+&t+r"#1 et&, Q+)m&"me!te#"% $r(te)!"% e%t2! &(!%tt+'"% $(r &(m5!"&(!e%&(m$#e"% 'e &"r5(!(1 9'r*0e!(1 (:)0e!(1 !tr*0e!(

- (tr(% e#eme!t(% e! me!(r $r($(r&*! &(m( %(!";+4re1 &(5re - 4*%4(r(,


4/12

INTRODUCCION

• C+"!'( #" e%tr+&t+r" 'e #" $r(te)!" %e 'e%(r0"!;"1 %e '&e 6+e%e e!&+e!tr" 'e%!"t+r"#;"'" - e%t( tr"e &(m( &(!%e&+e!&" #"$7r''" 'e #" "&t/'"' 5(#*0&", L" 'e%!"t+r"#;"&*! $+e'e#(0r"r%e $(r me'(% 4)%&(% &(m( e# &"#(r ( 6+)m&(% &(m( +!"/"r"&*! 'e $H1 (5%er/2!'(%e +!" '%m!+&*! e! #" %(#+5#'"'- #" 4(rm"&*! 'e +! &("0+#(, E%te m7t('( e% +t#;"'( $"r"'em(%tr"r #" $re%e!&" 'e $r(te)!"%,

• T"m57! %e $+e'e! 'e!t.&"r $r(te)!"% me'"!te e# +%( 'e

%+%t"!&"% 6+e "# $(!er%e e! &(!t"&t( &(! e##"%1 $r('+&e! +!"&(#(r"&*! e%$e&).&"< t"# e% e# &"%( 'e #" Re"&&*! 'e B+ret, E!e%t" t7&!&"1 %e "0re0" 9'r*:'( %*'&( - %+#4"t( &=$r&( " #"$r(te)!"< e# &(5re %e &(m5!" &(! e##" - t(m" +!" &(#(r"&*!$=r$+r",


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MATERIAL

MATERIAL SUSTANCIAS

> ? 4r"%&(% /"#e% ( ? t+5(% 'e e!%"-e > S(#+&*! 'e 0re!et!" "# @

> ? /"%(% 'e mL ( ? /"%(% 'e $#2%t&( 'e# > Re"&t/( 'e B+ret

> @ m(rter( &(! $%t#( ( +! &e!&er( > @ 9+e/(

@, me&9er( 'e "#&(9(# ( 'e 0"% >mL #e&9e

> @ 0r"'##" >@0 &"r!e

> @ $!;"% $"r" t+5( 'e e!%"-e

> @ er!0" 'e m# ( @ $$et" 'e m#

@ $;et" &(! "0+" 'e%t#"'"

@ e%$2t+#"


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PROCEDIMIENTO

• C(#(&" e! +! t+5( 'e e!%"-( @ m# 'e #" %(#+&*!'e 0re!et!" - "0re0" ? 0(t"% 'e re"&t/( 'eB+ret1 %e (5%er/"r2 +!" &(#(r"&*! ##" % e%t" !(

"$"re&e &"#e!te #0er"me!te,


7/12

PROCEDIMIENTO

• Muestras solidas :

• T(m" +! tr(;( 'e "$r(:m"'"me!te 0 'em+e%tr"

• De$(%)t"#( e! e# &e!&er( - trt=r"#( 9"%t"&(!/ertr#( e! +!" $"%t" 9(m(07!e"

• P2%"#( " +! /"%( 'e# N( m# 'e "0+" - '7"#(re$(%"r,

•

De #" %(#+&*! (5te!'"1 t(m" #" &"!t'"' !'&"'"$"r" &"'" $r+e5" #" &"!t'"' %+%t"!&" te%t0( -%0+e e# $r(&e'me!t( 'e%&rt( e! #(% te%t0(%$"r" &"'" &"%(,


8/12

PROSEDIMIENTO


9/12

PROCEDIMIENTO

• B. Muestras liquidas:

• N( e% !e&e%"r( tr"t"me!t( $re/(1 %e $+e'e!&"r e# $r(&e%( 'e 'e!t.&"&*!

&(rre%$(!'e!te


10/12

RESULTADOS

MUESTRA PRUEBA PARA PROTEINAS ( >

H+e/(

L" m+e%tr" %e /(#/( %e&"1 6+e'"!'( $e0"'" "#t+5( 'e e!%"-(, A# m%m( tem$( &(!%0+( +!&(#(r ##",

C"r!e Se !(t( #" $re%e!&" $(r e#&(#(r ##",

Le&9e Se !(t( #" $re%e!&" $(r e# &(#(r ##"1$er( %e t+5( 6+e "3"'r m"%

'e!t.&"'(r,


11/12

CUESTIONARIO,

• • E%&r5e #" "%.&"&*! 'e #"% $r(te)!"% $r(te)!"%

%m$#e% 9(#($r(te'(%1 $r(te)!"% &(!+0"'"%

9eter($r(te'(%1 - $r(te)!"% 'er/"'"%• • C+2# e% #" 4+!&*! 'e #"% $r(te)!"%• $"r" #" 'e%'r"t"&(! 'e +!" &e#+#",• A!(t" $(r #( me!(% "#me!t(% 6+e &(!te!0"!

$r(te)!"% '4ere!te% " #(% +%"'(% e! #" $r2&t&"• C"r!e%1 $e%&"'(1 $(##(1 9+e/(1 %"#&9&"%,

https://es.wikipedia.org/wiki/Holoprote%C3%ADnahttps://es.wikipedia.org/wiki/Heteroprote%C3%ADnahttps://es.wikipedia.org/wiki/Heteroprote%C3%ADnahttps://es.wikipedia.org/wiki/Holoprote%C3%ADna


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• Q+7 &"!t'"' 'e $r(te)!"% "$r(:m"'"me!te'e5e! 'e !0err

• " U! !3( 'e @ "3( 'e e'"',,,,,,@, 0r"m(%

• 5 U!" $er%(!" 'e @ "3(%,,,,,, @ 0r"m(%• & U!" $er%(!" 'e ? "3(% ,,,,,,,, 8 0r"m(%• C+2#e% %(! #"% /e!t""% ( 'e%/e!t""% 6+e te!e

e# !0err $r(te)!"% /e0et"#e% &(! re%$e&t( " #"%

$r(te)!"% "!m"#e% A&(m+#"! 5"%t"!te 0r"%" e!!+e%tr( !te%t!( 6+e e% '.&# $r(%e%"r,

actividad de laboratorio 8: "identificación de proteínas"

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