kiman jurnal presentasi joss

8/18/2019 Kiman Jurnal Presentasi Joss

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AUGUSTINE LUTVIANTI PRAMONO

AGUNG FEBRIAN RAMADANI

TIKA YASMIN RAHMAYANTI

Ke


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HIGH PERFORMANCE

LIQUIDCHROMATOGRAPHIC

UNTUK MENENTUKANCIPROFLOXACIN

HYDROCHLORIDE DAN


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METODE PENETUAN CIPROFLOXACINEHIDROKLORIDA DAN ORNIDAZOLEDALAM PLASMA DARAH

Metode :

1. Pemb!t!" l!tt!" #to$% d!" l!t&#t!"d!&

C'(&o)o*!$'" +'d&o%lo&'d! , o&"'d!-ole , t'"'d!-ol d'#'!(%!"d'l!&t%!" d!l!m met!"ol d!" d'b!t l!&t!" #to% m!#'"/m!1000 2 ml. l!&t!" #t!"d!&t d!&' 100 m 2 ml $'(&o)o*!$'"+'d&o%lo&'d! , o&"'d!-ole d!" t'"'d!-ol d'#'!(%!" "t% d'l#'l!&t!" #to% d' 3!#e e&!% . L!&t!" Sto$% d'#'m(!" (!d! #+%!m!& 45 6 C


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4. Pe'!(!" #!m(el (l!#m!D'm!#%%!" l!&t!" #to% $'(&o)o*!$'" +8d&o$lo&'d! d

o&"'d!-ole de"!" olme 0, 1, 1., 4, 4., ;, ;., d!" <

%e d!l!m 1 mL t!b" #e"t&'3e d!" d't!mb!+%!" %e(l!#m! beb!# ob!t "t% membe&'%!" #t!"d!& %!l'b&!#=t'd!% !d! $'(&o)o*!$'" d!" o&"'d!-ole 8!" d't!mb!+%!100 , 10 , 400 , 40 , ;00 , ;0 , d'#'m(!" %o"#t!". U"t% K!%o"t&ol =?C> d'"!%!" #!m(el 8!" tel!+ d'#'!(%!" d!l(l!#m! "t% %ed! ob!t d!l!m %'#!&!" %o"#e"t&!#' 400

;00 , 8!" d'!d% dem'*e& #el!m! 1, me"'t d!" d'#e"t&'3!#' (!d! 5000 &(#el!m! 10 me"'t . F!#e o&!"'% %emd'!" d'!(%!" #!m%e&'" d' +ot(l!te. M!##! &e#'d d'l!&t%!" de"!" 1 mle&!%. A"!l'#'# '"' d'l!%%!" (!d! #'#tem HPLC.


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;. Ko"d'#' %&om!to&!@ F!#e e&!% te&('l'+ #eb!!' $!m(&!" !#eto"'

met!"ol , !'& d!" t&'et'l!m'"! d!l!m &!#'o =


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. St!b'l't!# l!&t!" #to% d!&' +'d&o%lo&'d! $'(&o)o*!$'" d!"o&"'d!-ole d'(e&'%#! (!d! #+ %!m!& #el!m! !m. F&ee-e/t+!B #t!b'd'te"t%!" (!d! t'! %o"#e"t&!#' ?C =&e"d!+, #ed!", t'"'> #etel!+(embe%!" =/40 6 C> d!" (e"$!'&!" #el!m! t'! #'%l# d!" d'b!"d'"%!de"!" "'l!' "om'"!l. #t!b'l't!# #t!"d!& %ed! ob!t 't d'"'l!' "t% ?C=&e"d!+, #d!", d!" t'"'> de"!" memb!"d'"%!" de"!" "'l!' "om'8!" d'#'m(!" (!d! #+ %!m!& #el!m! 14 !m. Pe"!&+ (e"8'm(!"!#!m(el d'"'l!' de"!" memb!"d'"%!" #!m(el ?C 8!" d''"e%#' #etel!

(e'!(!" d!" #'#! 8!" te&t'"!l #etl!+


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PENENTUAN OGAT/OGATAN DARI GKELAS TERAPI OLEH EKSTRAKSI FASE PA

MASSA KROMATORAFI/TANDEM CAIR SPEKTROMETRI DI AIR LIMGAH LIMGA


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METODE EKSTRASI PADATSPE (Solid-Phase Extraction)

SPE adalah proses pemsaha! dma!a se!"a#a "a!$ %erlar&% a%a& %ers&spe!s dala

'ara! dpsah(a! dar se!"a#a la! dalam 'amp&ra! ses&a de!$a! s)a% )s( da!

a!alss me!$$&!a(a! e(s%ra(s )ase pada% &!%&( me!$(o!se!%ras(a! da! mem&r

&!%&( a!alss*

SPE ,&$a le+h ser!$ d$&!a(a! darpada LLE -L.&d/L.&d E0%ra'%o!1 (are!a

+e+erapa (e&!$$&la! "a%& 2

T!$(a% pem&lha! "a!$ %!$$ Me!!$(a%(a! sele(%3%as4 spes)(as da! reprod&(%)%as

Me!$elm!as em&ls

Sed(%!"a pelar&% or$a!( "a!$ d$&!a(a!

5a(%& preparas sampel pe!de(

M&dah pe!$oprasa!!"a da! (em&!$(!a! o%oma%s


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Metode '"' mem"%'"%!" #'mlt!" e%#t#e"8!B! ob!t/ob!t!" de"!" e%#t&!%#' 3

(!d!t =SPE> me""!%!" !te O!#'#(!d! (H . !"!l't %emd'!" d''de"t'@%!#%!"t't!t'3 d'te"t%!" ole+ #(e%t&omet&' %&om!to&!@ $!'&/t!"dem =LC/MS/MS>

me""!%!" (em!"t!!" &e!%#' bebe&=MRM>. Kete(!t!" metode, d'+'t" #eb!de'!#' #t!"d!& &el!t'3 =RSD>, be&%'#!& 0,;


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P&o#e# !l'd!#' (&o#ed& !"!l'#'#, m!#'" J m!#'" #e"8!Bde"!" MRM de"!" me""!%!" d! (&e$o& t'"' t&U"t% me"e"t%!" (eml'+!" e%#t&!%#', %o"#e"t&!#' m!t&'l'mb!+ #ebelm d!" #etel!+ e%#t&!%#' d'b!"d'"%!". L'"e!metode '"' d'e!l!#' me""!%!" &m!+ #!%'t !'& l'mb!+

d'bb+' !"!l't. D!t! e%#(e&'me"t!l d'le"%!(' mode l'"e! d'/(e"el't'!" %'#!&!" %o"#e"t&!#'.

Kete(!t!" metode '"' d'"8!t!%!" #eb!!' #t!"d!& de'!#' &=RSD> d!&' (e"%&!" b!t!# dete%#' metode =MDL> d!" m%!"t't!t'3 b!t!# 'o" =M?L> 8!" tel!+ d'de@"'#'%!" d!" d't#eb!!' ml!+ m'"'mm !"!l't te&dete%#' de"!" &!#'o #'

I"te&(&et!#' +!#'l 8!" d'd!(!t%!", mel'(t' (emb!+!#!" S!"!l'#'# LC/MS/MS. U"t% me"$'&'%!" #'3!t m!t&'%# %om(le%#!%'t l'mb!+, d'%& d!&' (H, %o"d%t''t!# d!" te&l!&t o&%!"d"!" %!&bo" 8!" d'l!%%!", &!t!/&!t! 8!", 5.4, 1.140 ((m, m!#'"/m!#'".


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Pe"!&+ (H (!d! e@#'e"#' e%#t&!%#' d'(el!!&' de"me"8e#!'%!" "'l!' (H #!m(el (!d! (H 4.0,


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Seb!+ "'l!' (eml'+!" #e#!' de"!" &!t!/t'! l!"!" d!&' 1 2 L d!&' #e"8!B! 8!" d!l!m !'& l'mb!+ &m!+ #!%'t #ebelm d!" #e%#t&!%#'. RSD: &el!t'3 #e%'t!& 5, d!" #e"#'t'=b!t!# dete%#', ;4 " 2 L>, #!"!t b!'%, (em&e"d!+ !d! %elem!+!" "t% (e"e"t!" d'!&!"'t'd'".


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PENGU6IAN DENGAN HPL7 U"t% me"o(t'm!l%!" (em'#!+!" %&om!to&!@, d' !B!l (

d'' 3!#e mob'le 8!" be&bed! 8!" te&d'&' d!&' !'& d!" me!#eto"'t&'l !t! $!m(&!" !#eto"'t&'l d!" met!"ol #eb!!' o&!"'% d!" (!d! be&b!!' (H, #eb!!' 3!#e d'!m !d't'3 !m!#et!t mM d!" !#!m 3o&m!t 0,1 d!l!m !'& M'll'? d''.Pe""!!" !mmo"'m !#et!t (!d! %o"#e"t&!#' b'!#!"8! !"t!&! 4 #!m(!' 40 mM, #eb!!' !d't'3

d!l!m 3!#e e&!%, Pem'l'+!" 3!#e e&!% d'b!t "t% me"(!l'" #e"8!B! "t%

Dem' %e#ede&+!"!!" metode. Me#%'(" (em'#!+!" le"%!d'(e&l%!" "t% #ele%t'3 d!l!m dete%#' MS/MS, mm"8!me"'"%!t%!" (e"dete%#'!" d!" me"&!"' e3e% (e"e%!"


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METODE PREPARASI SAMPEL

Ko!sep dasar me%ode preparas sampel dalam ,&r!al ! adalah &!%&

ma%r(s !"a%a me!,ad 'o'o( &!%&( a!alss sampel* Proses ! hampr s per&+aha! !%era(s se!"a#a de!$a! l!$(&!$a! (ma se(%ar* I!%era(s ! d%

s)a% )s(a da! (ma a!al% ser%a ma%r(s4 da! hal %& sa!$a% mempe!$ar&h

%e(!(/%e(!( persapa! sampel "a!$ +er+eda da! me%ode a!alss ser%a

reprod&(%)%as* Oleh (are!a %& (ara(%ersas se'ara )s(o(ma %erhadap (ead

sampel sa!$a% pe!%!$ a$ar (%a me!$er% !)ormas %erhadap s)a% )s(a da! (m


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1. SPE (Solid-P!"# E$%&!'%io)

*. Solid-P!"# Mi'&o#$%&!'%io (SPME)

+. S%i&-,!& So&%i# E$%&!'%io (S/SE)

0. M#,&!# E$%&!'%io

2. Li34id-P!"# Mi'&o#$%&!'%io (LPME)

5. S4#&'&i%i'!l Fl4id E$%&!'%io (SFE)

6. P""4&i7#d Li34id E$%&!'%io (PLE)

8. M!%&i$ Solid-P!"# Di"#&"io (MSPD)

9. Di"#&"i# Solid-P!"# E$%&!'%io (DSPE)

1:.Ul%&!"oi' E$%&!'%io (USE)

11.Mi'&o;!#-A""i"%#d Sol#% E$%&!'%io (MASE)


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KESIMPULAN

Perl& d(e%ah& +ah#a preparas sampel mer&pa(a! s&a%& proses "a!$ sa!$a

&!%&( me!e!%&(a! hasl a(hr da! a!alss "a!$ a(a! d$&!a(a!* Sema(! me!!

pe!$$&!aa! o+a%/o+a%a! saa% !4 le+h dar +e+erapa prosed&r preparas sampe

d(em+a!$(a!* Da!%ara!"a "a%& SPE "a!$ ma!a sa!$a% pop&lar &!%&( a!alss

o+a% da! %elah me!,ad me%ode "a!$ sa!$a% pe!%!$ d la+ora%or&m d sel&r&h d&

%erse+&% ,&$a %elah me!$$a!%(a! %e(!(/%e(!( "a!$ le+h %&a* Pe!$em+a!$a

SPE 'epa% se(al da! %elah me!$alam +a!"a( per+a(a!* Salah sa%&!"a "a%& MIma!a s)a%!"a le+h spes)( da! le+h sele(%)4 pe!$$&!aa!!"a m&!$(

(edepa!!"a a(a! sema(! l&as ,&$a %er&%ama dalam a!alss )ore!s(4 (l!s4 )arm

+o(ma* Lal& &!%&( SPME da! E(s%ra(s Mem+ra! me!,ad al%er!a%) "a!

me!ar( &!%&( SPE dalam hal sampel 'ar4 seda!$(a! PLE4 MSPD4 da! MASE

al%er!a%) "a!$ %epa% &!%&( o+a%/o+a%a! "a!$ mel+a%(a! sampel pada%*

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