immune parametres of mussels treated with prestige fuel oil
DESCRIPTION
Immune parametres of mussels treated with Prestige fuel oil. M. Camino Ordás, Joan Albaiges, Josep M. Bayona, Amando Ordás and Antonio Figueras. Instituto de Investigaciones Marinas (Vigo) Centro de Investigación y Desarrollo (Barcelona) Misión Biológica de Galicia (Pontevedra). - PowerPoint PPT PresentationTRANSCRIPT
Immune parametres of mussels treated with Prestige fuel oil
M. Camino Ordás, Joan Albaiges, Josep M. Bayona, Amando Ordás and Antonio Figueras
Instituto de Investigaciones Marinas (Vigo)Centro de Investigación y Desarrollo (Barcelona)Misión Biológica de Galicia (Pontevedra)
Inespecific system no antibody production
Cellular defense Humoral defense
• Phagocytosis• Respiratory burst• NO production
• Lysozyme• Lectins• Prophenoloxidase
SERUM SOLUBLE FACTORSHEMOCYTES
External barriers
Hemocytic infiltration
Immune system of bivalve molluscs
contaminants
depression in immune system
infection by pathogens
MORTALITYimportant economical losses in Galicia, one of the main Mediterranean mussel producers worldwide
To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.
Objective of this work
contaminants
depression in immune system
infection by pathogens
MORTALITYimportant economical losses in Galicia, one of the main Mediterranean mussel producers worldwide
To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.
Objective of this work
contaminants
depression in immune system
infection by pathogens
MORTALITYimportant economical losses in Galicia, one of the main Mediterranean mussel producers worldwide
To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.
Objective of this work
contaminants
depression in immune system
infection by pathogens
MORTALITYimportant economical losses in Galicia, one of the main Mediterranean mussel producers worldwide
To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.
Objective of this work
contaminants
depression in immune system
infection by pathogens
MORTALITYimportant economical losses in Galicia, one of the main Mediterranean mussel producers worldwide
To assess the influence of the Prestige fuel oil on the immune response of Mediterranean mussel.
Objective of this work
Methodology
+1 kg fuel +2 kg fuel control
Monthly sampling during four months
•Histology.
•Immune parametres: Cellular: hemocyte viability, phagocytosis, NO production, chemiluminescence (CL).
Humoral: protein concentration and lysozime activity.
•PAH concentration in water and mussel tissue (gills and digestive gland).
tank 1 tank 2 tank 3
Results
- No granulocytomes or neoplasia
- Mytilicola in 68% of sampled animals
1. HISTOLOGY
2. IMMUNE PARAMETRES
df Viab. Phagoc. CL NO Protein Lisoz.
Month (M) 3 0.008** 252.75 0.0003** 5273.16** 0.043 70.41**
Treatment (T) 2 0.002 266.59 0.0001* 536.08 0.081* 54.98**
M T 6 0.008** 143.37 0.0002** 2611.54* 0.010 24.92*
Error 36 0.002 146.12 0.0000 1015.16 0.020 8.65
2.1. Mean squares (34 factorial ANOVA):
•No significant differences in phagocytic activity and significant
differences in protein concentration.
•Remaining parametres: interaction between month and treatment.
2.2. One way ANOVA (without taking into account the
sampling month): significant differences in protein
concentration and lysozyme activity.
2.3. Least square means:
Treatment Viab. Phagoc. CL NO Protein Lysoz.
2 kg 0.220a 11.080a 0.015a 52.770a 0.242a 7.507ab
1 kg 0.219a 13.890a 0.013a 50.120a 0.283ab 9.636a
0 kg 0.236a 19.108a 0.010a 61.200a 0.381b 5.943b
Means followed by the same letter in a column are not statistically different according
to the Waller-Duncan k- ratio t Test.
3. PAH CONCENTRATION
0,00
20,00
40,00
60,00
80,00
100,00
120,00
140,00
gill 1 kg gill 2 kg dig. gland1 kg
dig. gland2 kg
3 MP
2 MP
9 MP
1 MP
Prestige fuel oil
0
200400
600
8001000
1200
14001600
1800
Similar methylphenantrene profiles in mussel organs
and Prestige fuel oil real exposure to the fuel.
0,00
500,00
1000,00
1500,00
2000,00
2500,00
3000,00
3500,00
mes 1 mes 2 mes 3 mes 4
1 kg2 kgcontrol
0,00
5000,00
10000,00
15000,00
20000,00
25000,00
mes 1 mes 2 mes 3 mes 4
1 kg2 kgcontrol
TPAH gill
TPAH dig.gland
•Higher TPAH concentration (ng/ g) in digestive gland than in gill.
•Low TPAH conc. in control mussels.
•Excepting gill from the first month, organs treated with 2 kg of fuel show higher TPAH conc. than 1 kg- treated ones.
REGRESSION PROCEDURE:
• Model: [contam]=0 + 1 MONTH + 2 TREATMENT
• Number of PAHs depending on each independent variable:
^
only treatment
only month
treatment + month
no dependent
GLAND 9 4 7 5GILL 3 - 1 21WATER - - - 25
For comparisons between [PAH] and immune parametres, it would be more appropriate to use data from digestive gland, since [PAH] in gill is independent of our experimental design.
Comparison between gland [PAH] and immune data (difficult due to
the experimental design).
Future analysis
Muchas gracias