celulas derivadas de placenta a termino

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Revista de HematologaVolumen 12, nm. 4, octubre-diciembre 2011276

Artculo de revisin

Rev Hematol Mex 2011;12(4):276-286

Utilidad teraputica potencial de las clulas madre de la membranaamniticaCarmen L Insausti,* Miguel Blanquer,* Mara J Majado,* Alfredo Insausti,** Jos M Moraleda*,***

RESUMEN

Desde los primeros estudios de clulas madre obtenidas de la membrana amnitica se ha avanzado mucho en el conocimiento de las

caractersticas de estas clulas, sus mtodos de obtencin y procesamiento, as como de su potencialidad. Numerosos ensayos preclnicos

sugieren que las clulas madre de la membrana amnitica podran ser tiles en la regeneracin de una serie de alteraciones tisulares.

estrecha colaboracin entre la investigacin bsica y los clnicos para que la terapia con clulas se haga realidad en la prctica diaria.

Palabras clave: membrana amnitica, clulas madre, clulas de la membrana amnitica, clulas epiteliales, clulas mesenquimales,terapia celular.

ABSTRACT

handling methods and potential of these cells. A lot of preclinical evidence suggests that human amniotic derived cells may be useful for

of amniotic derived cells are due to differentiation of the transplanted cells themselves or to paracrine actions on the surrounding host tissue

clinical reality, it is fundamental a close collaboration between basic scientists and clinicians.

Key words:Amnion, amniotic membrane, stem cell, amniotic epithelial stem cell, amniotic mesenchymal stem cell, Cell therapy.

* Servicio de Hematologa y Hemoterapia. Unidad de TerapiaCelular. Hospital Universitario Virgen de la Arrixaca.

** Servicio de Oftalmologa. Hospital Universitario La Paz, Madrid.*** Universidad de Murcia. Murcia, Madrid.

Correspondencia: Dra. Carmen L Insausti. Servicio de Hematologa

y Hemoterapia, Unidad de Terapia Celular. Hospital UniversitarioVirgen de la Arrixaca. Ctra. Madrid-Cartagena. El Palmar 30120,Murcia. Correo electrnico: [email protected]: octubre 2011. Aceptado: noviembre 2011.

Este artculo debe citarse como: Insausti CL, Blanquer M, MajadoMJ, Insausti A, Moraleda JM. Utilidad teraputica potencial delas clulas madre de la membrana amnitica. Rev Hematol Mex2011;12(4):276-286.

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277Revista de HematologaVolumen 12, nm. 4, octubre-diciembre 2011

Utilidad teraputica potencial de las clulas madre de la membrana amnitica

fuera de sta se encuentra una capa dispersa de clulas

3,4

- human Amniotic Epithelial cells, - -

human Amniotic MesenchymalStem or Stromal cells -

- -

invitro

Tumor Necrosis Factor) Transforming factor) (Epidermal Growth Factor Hepatic

growth factor Keratinocytesgrowth factor -

Usos de las hAEC y hAMSC en modelos animales

- -

Brain derived netrophic factor y NT-3:eurotrophin-3) 28

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L Insausti C y col.

Cuadro 1.Trasplante de HAEC o HAMSC en modelos animales de enfermedades

Enfermedad Animal Evolucin Referencias

Enfermedad de Parkinson

Contusin del cordn medular

Obstruccin arterial cerebral media

Tejido heptico

Fibrosis heptica

Infarto cardiaco

Diabetes

Fibrosis pulmonar

Tejido cartilaginoso

Enfermedades del odo

Pared abdominal

Isquemia de extremidades

Rata

Ratn

Rata

Mono

Rata

Rata

Ratn

Conejo

Ratn

Rata

Ratn

Ratn

Ratn

Rata

Cobayo

Rata

Ratn

Supervivencia celular. Sntesis y secrecin de catecolaminas y factores

-

paminrgicas.

Efecto neuroprotector y neurognico.

Mejora clnica. Expresin de marcadores neurales

Integracin celular sin reaccin inmunitaria

Supervivencia celular. Expresin de marcadores neuronales. Disminucin

del rea infartada. Mejora clnica

Integracin en tejido heptico. Expresin de marcadores hepticos.

Integracin heptica. Expresin de receptores.

Supervivencia celular. Expresin de marcadores cardiacos. Mejora de

la funcin cardiaca.Correccin de hiperglucemia. Integracin celular en el hgado y el pncreas

Produccin de protenas surfactantes A, B, C y D.

Expresin de genes, receptores, colgeno tipo II y aggrecan

Localizacin en la regin supra-limbal, regin supra-espiral en la scala

vestibuliy en la regin subcentral del ligamento espiral en la scala timpani.

Supervivencia durante 3 semanas. Expresin de marcadores.

Integracin celular. Mejora de cicatrizacin. Reduccin de la incidencia

y tamao de hernias incisionales.

27,28,

29

30

31

32

33

5,35,37,39

36

40

41,42

44

45,46

18

47

48

49

- - 3,4-Dihydroxyphenylaceticacid: Homovanilic acid) en el striatum del

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-31

32 -

33tras el tras-


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34 fuente de clulas para la terapia celular en ictus, una

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36 Low density lipoprotein receptor

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38 - -

present tripsina humana.

en

4), trasplan-

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2

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18

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in vitro - - invivo - lascala vestibuli espiral en lascala timpani,

48 - Amniotic multipotentcells -

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- -

in vivo de 18,33,41 -

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enfermedades.

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Cuadro 2.Publicaciones recientes acerca de la indicacin clnica de la membrana amnitica

Aos Ttulos Referencias

2000

2001

2003

2004

2005

2006

2007

2008

2009

2010

Reconstitution of damaged corneas by transplantation of autologous limbal epithelial cells

Amniotic membrane graft in ocular surface disease. Prospective study with 31 cases.

Successful reconstruction of damaged ocular outer surface in humans using limbal and conjuctival stem cell

culture methods.

The Amniotic membrane in ophthalmology

Amniotic membrane use in ophthalmology

Amniotic membrane transplantation for ocular surface reconstruction.

Ultrastructural analysis of in vivo expanded corneal epithelium on amniotic membrane

Amniotic membrane transplantation for treatment of symblepharon in a patient with recessive dystrophic epi-

dermolysis bullosa.Amniotic Membrane transplantation in palliative treatment of bullous keratopathy

Clinical outcome of autologous cultivated limbal epithelium transplantation

Amniotic Membrane Transplantation

Use of amniotic membrane graft and corneal transplantation in a patient with bilateral keratomalacia induced

by uncontrolled phenylketonuria

Use of amniotic membrane transplantation in the treatment of venous leg ulcers.

Amnia for intractable skin ulcers with recessive dystrophic epidermolysis bullosa: report of three cases.

Amniotic membrane transplantation for the management of corneal epithelial defects: an in vivo confocal mi-

croscopic study.

Manufacturing of human placenta-derived mesenchymal stem cells for clinical trials

Amniotic membrane transplantation in the treatment of persistent epithelial defect on the corneal graft.Use of amniotic membrane transplantation in isolated conjunctival Bowen disease: a case report

Dried irradiated human amniotic membrane as a biological dressing for facial burns--a 7-year case series

Amniotic membrane induces epithelialization in massive posttraumatic wounds.

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68

69

55

56

64

65

63

59

70

57

60

71

72

66

75

6761

73

74

experiencias en pacientes.

CONCLUSIN

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L Insausti C y col.

- Good Manufacturing Practice -

Agradecimientos

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celulas derivadas de placenta a termino

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