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Nutrigenómica y

Nuevos Ingredientes

VAL.-LEU-*SER-GLU-GLI-GLU-TRI-GLN-LEU-VAL10-LEU-HIS-VAL-TRI-ALA-LIS-VAL-GLU*-ALA-*ASP20-VAL-ALA-GLI-HIS-GLI-GLN-ASP-ILE-LEU-ILE30-ARG-LEU-FAL-LIS-SER*-*HIS-PRO-GLU-TRE-LEU40-GLU-LIS*-FAL-ASP-ARG-FAL-LIS-HIS-LEU-LIS50-*TRE-GLU-ALA-GLU-MET-LIS-ALA*-*SER-GLU-ASP60-LEU-LIS-LIS-HIS-GLI-VAL-TRE-VAL-LEU-TRE70-ALA-LEU-GLI-ALA-ILE-LEU-LIS*-LIS-LIS-GLI80-HIS-HIS-GLU-ALA-GLU-*LEU-LIS-PRO-LEU-ALA90-GLN-SER-HIS-ALA*-TRE-LIS-HIS-LIS-ILE-*PRO100-ILE-LIS-TYR-LEU-GLU-FAL-ILE-SER-GLU-ALA110-ILE-ILE-HIS-VAL-LEU-HIS-SER-ARG*-HIS-PRO120-GLI-ASP-FAL-GLI-*ALA-ASP-ALA-GLN-GLI-ALA130-MET-ASN-LIS-ALA-LEU-GLU-LEU-FAL-ARG-LIS140-ASP-ILE-ALA-ALA-LIS-TIR-LIS-GLU*-LEU-GLI150-TIR-GLN-GLI153

Plataforma Fundamental Para Abordar las ECNT

Foods For Specified Health Use

(FOSHU)

Japanese Ministry of Health, Labor and Welfare (MHLW) defined "Foods for Specified Health Use (FOSHU)" as foods officially approved to claim their physiological effects on the human body.

Seal of approval for FOSHU

Categories of Dietary/Food Supplements

? So-Called Health Foods

? Foods with Health Claims

# Food for Specified Health Uses(FOSHU)

• Existing(Original) FOSHU

• Standardized FOSHU

• Qualified FOSHU

• FOSHU With Disease Risk Reduction Claim

# Food with Nutrient Functional Claims(FNFC)

Copyright c JHNFA

REQUERIMIENTO UMBRAL VERSUS DAÑO POTENCIAL

Bisulfite genomic sequencing

Confirm these sequences featured DNA methylation

Genomic DNA

bisulfite

C U

mC mC Clone sequencing

C T

mC C

methylation

unmethylation

Maximum and Minimum Levels : Minerals

NUTRIENTS Maximum level Minimum level

Calcium 600 mg 210 mg

Iron 10 mg 2.25 mg

Zinc 15 mg 2.1 mg

Copper 6 mg 0.18 mg

Magnesium 300 mg 75 mg

Maximum and Minimum Levels : Vitamins NUTRIENTS Ｍａｘｉｍｕｍ level Minimum level

Vitamin A

（β-Carotene）

650μg

（7,200μg）

135μg

（1,620μg）

Vitamin D 5.0μg 1.50μg

Vitamin E 150 mg 2.4 mg

Vitamin B1 25 mg 0.3 mg

Vitamin B2 12 ｍg 0.33 ｍg

Niacin 60 ｍg 3.3 mg

Biotin 500 μg 14 μg

Pantothenic acid 30 mg 1.65 mg

Vitamin B6 10 mg 0.3 mg

Folic acid 200 μg 60μg

Vitamin B12 60 μg 0.6 μg

Vitamin C 1000 mg 24 mg

Nutrigenomics and Nutrition

Nutrigenomic Nutrition Diet Metabolomic

LMWC Nutrient-gene int.

Nutrient-gene interaction: 1. Direct interaction (induce gene expression) 2. Epigenetic interaction (gene exp. Chronically altered. 3. Genetic variations (S.N-polymorphisms (SNPs).

LOW MOLECULAR WEIGHT COMPOUNDS IN MARINE FOODS

• PEPTIDES < 100kDa • FREE AMINOACIDS • NUCLEOTIDS • INMUNOGLOBULINS • LMW-GELATIN • CAROTENOIDS • ANTIOXIDANTS

“FUNCTIONAL FOOD” vs. TOKUHO

Food Functional Food TOKUHO (FOSHU) Marine Food*

(Tertiary I. Nutrition (Primary function) function) (inmune, nerv. etc.) II. Sensory satisfaction (flavor, aroma), (Secundary function) III. Fortification Food that claims Food product modulation of its function approved to claim physical system (Not necessarily its clinically proven clinically proven) function Ref: H. Hosoya

Copyright JHNFA * Marine cold water fish

Fatty acids Evidences •Altered expression of Transtyretin, -synuclein, calmadulins ( synaptic plasticity and learning)

Blood Brain Barrier

I, Cu, Zn, Se, Fe

Phospholipids (GM 70%)

Neural membrane, 50% DHA (sn-1,3)

C22:6n-3, sn-1,3

Lysoph. (70% GM)

“Phospholipids are the centre life and chemical soul of all bioplasma whatsoever that plants as

well animal (Pepeu et al., 1990). Comparison between TG and PL-feed groups indicated that only PL supplementation increased brain DHA, Werner

et al., 2004.” sn-3

sn-1 sn-2

Environmental

¿ Porqué la Nutrición Intrauterina y hasta el año de Edad es la más

Importante?

EL CRECIMIENTO Y DESARROLLO CEREBRAL ALCANZA SU MAYOR VELOCIDAD EN LA VIDA INTRAUTERINA

•Durante la formación del cerebro 250.000 neuronas aparecen cada minuto •¡ En la vida intrauterina 2 de 3 neuronas mueren por apoptosis al no lograr conexiones con otras neuronas (Gradiente de Vulnerabilidad) ! •Al nacer casi todas las neuronas del cerebro adulto están presente.

JHNFA

Vulnerabilidad v/s Alto Requerimiento Energético

“El Peak de Neuronas Ocurre a las 10-18 Semanas de Gestación”

Head injury

Experimental Parkinson disease Schizophrenia

Alzheimer disease

Prion diseases

Spinal cord injury

Ischemia

Multiple sclerosis

PLA2

isoforms

Farooqui et al., 2006.

TOKUHO (FOSHU)

LMWC

LYSOPHOSPHOLIPS

DHA sn - 1,3

GLUCOSE

I

Cu

Zn

ARA sn – 1,3

Se

PA sn - 2

Sirt-1

Sch-9

Fe

Glycine

Glutamate

Na/k pump Ca

Rec-A

Blood Brain Barrier

Altered expression of Transtyretin, -synuclein, calmadulins ( synaptic plasticity and learning)

LMWC, Phospholipids, AG-PUFAn-3, Antioxidants,

Vitamins, Minerals in fish bone

Retinol Phospholipids(PC,PE,PS)

DHA

Proteins (LMWC)

Zn-Ca-Se-Mg

Proteins: 40%

Minerals: 47%

Collagen

Keratin (LMWC)

Minerals

Peptides as antioxidants

(3-16u.) Tyr-met-his-lys-

trp.

LMWC of Marine Concentrate PM:26132-02/11/2011

93,18%<100kDa

LMWC of Marine Concentrate PM:26132-02/11/2011

Antartic ExpeditionAntartic Expedition

n-6/n-3 n-6/n-3 ratio ratio (2001-2003)(2001-2003)

0

2

4

6

8

10

12

P.Gut. E. Guz. E.Oliv. R. Jor.

Dic. 2001,n-6/n-3

En. 2003,n-6/n-3

Everest Everest ExpeditionExpedition

n-6/n-3 n-6/n-3 ratio ratio (2003-2004) A. L.C. (2003-2004) A. L.C. .

0

1

2

3

4

5

6

EPA C20:5w3 DHA C22:6w3 W6/W3

Ene.2003

Jul.2003

Feb.2004

Criogenic Elements(Antartic exp., 2002 and Everest exp., 2004)

Criogenic Elements(Antartic exp., 2002 and Everest exp., 2004)

ANTI-FREEZE PROTEIN

(Diagram)

ANTI-FREEZE PROTEIN

(Diagram)

NH-Ala-Ala-Thr-Ala-Ala-Thr (NH-Ala-Ala-Thr-Ala-Ala-Thr ( ProProAlaAla -Ala-Thr -Ala-Thr ) ProPro

AlaAla - Ala-COO- Ala-COO

0

50

100

150

200

Pig Nº 7 41 64 55 2,549

Pig AG. 65 47 78 4,878

Italic Pig 198,8 19 81 6,877

LDL HDL Triglic. n6/n3

PROYECTO -AG

ORAC hyro (umole/g)

ORAC Value

FeO2 FeO3 and Free radicals

MARINE BEEF

Productos Con VTA de Salmón Transglutaminasa y Bacterias Probióticas

Salmón Fermentado y Ahumado en Frío con Bact. Probióticas y TG.

Salmón Ahumado en Frío con TG + CoP. en Horno Vemag Climamat-F

STARTER PROBIÓTICO

Vielen Dank für Ihre Aufmerksamkeit! Thank you for your attention!

¡Muchas gracias por vuestra atención! ご清聴ありがとうございまし

(Go seichō arigatō gozaimashita)