utr? ?r'r {tun n mo? fio ei'u inrtr nrtpr (diagnosis of … p62-69.pdf62...
TRANSCRIPT
62 x'rrar'r:#a'rrry{nucn6{a{ &?r. fld o ariu{{ 2 nrn0larr - fiueroru 2sg9
utr? ?r'r {tun r : n mo? fio ei'u inrTr nrTpr
(Diagnosis of Bovine Tuberculosis)
urruud r.rd'iflulsnrr':.J:snryr: mtrrurtfit'
oilnu'l
i'ruI:oTn tijuT:n 6o qiod etr;ry drirtd'ln firJo rarnr.r qunrn o d'r.: r{o;.: ornr:r arl:nrin'lilrsiud'efin fid'fl:rnr:qrurh lulourusrrur:nvirlri'rfieroi'rurua-nr*ulA lrro"rcutosis Masritis) uo;yrhtri
A r ^^..4.. - X .-_.61_ q Z X e junt'Jnauilnon.: tflmqott0e;tuttoaonmt.:nrltru[o rflult! oeo't:y ilmgl:s rirrEo rr:nqun{01n-.-.-.4 e ( Y a v iu. t-j:^.J^..^-.rt^,q_l-,^-. , ad , qe:3:.J:"Jfl:Jllilq.ttn;u'tutJ q.lururJfr'[oil1a1nleryroorgonn;'Lrj'[ci'p.irunrltSouodr.:nnii srtr:nriotrit_g
rfiqirilI:ntuou'ld'dtfia'jr'[oi"jrrflul:n#o{6onu (Zoonosis) iirirti'ty6nl:or.rdt uoiiu:]oiriu iffitfluv f i rl-,+ d o di-e-.-.u I ^ - A. y 4 u n
tn'l:orlo?uaau toun? lild0 nr:flofrrtuai6rdutrir Lufrrurll 6.rf.lfrnlrrutrlunr:o:ro?uQflun, a a Z- ^'[ristfn o-nvitu':rfiezunurnlfio (false positive) unrernnlrfio (tatse negative) 'lri'rsruo :]aoliu6t"[d'finr:ilieluruutmr.:1unr:n:':oifiosi'u rdahi'finrrr:trrrnrnrrr:o1r,n.ru.ndu r:iu nr:t.i'ryrnfin fi d orfieruri'u 6 rdu ro In:u (potymerase chain reaction, PCR with DNA probe) n't:etr?ouneJilr-6urflofll:ou(gamma-interferon) lurfioqleuid'lmnfinr 6'la ro (enzyme immuno assay, EtA) n1:?lodounnyn:on6'laoi'(glutareldehyde test) utrn.r,*.r,#, (cutture) rfluq"u un"..ud,Jrss{sincrauor,r*rrrr*rn..,.rfitu urssrutunr:o:roifiod'udosrir'lrlt'ilunr.::JfiriG un;fin':rrd.i rdofi orfi rrno
1 Y a - ( Y d,A' unfr nr*r nqr rn rvr rl'i'uilfi o n rwfr'or uvr vr usr oqrf ruuri n ur n-ur aurrriu2 ^ dA u c' nrnrtrs5:1r rJr nilsd-o?rrylyl ufl1flnf ruurimutn''u:r ouuriu
64 x1r6{1i#ax rryryr rlgrl6{a{ ers. fl d o *u-ud 2 nrngrail - frr.lmerru 2s39
nrtfuTrrnnfrn fi fic?lou [a luflr
6tQ)4a'lt 5?ilnil 9t
tunril"vrnfin fi 6 or{ tdonr:o:rovt & ^ a a d a tr?ilQilu[:nuu ryrnun ?i t 0'r: fl0?u?iln']Tly{il
.a<iXalu2141 A.: fl't U q IAU tO l1Q't tl^t't; torlO.: nU tra'lO
XA,acrAacAtt0141{.i fl?u 9t tou [0 [y{TU n0 q toil t0 71
. & d q. ,a a e d d A dflT',r.]ljuty{0tt[uTUlJtylu:Jn! 0 tou ta ljaittoyr
ad(vffl.:aTlAUil.tQ1n9.J1Un1Tm1 ?i t A1T illttn1 IOU
6 r6u ro }nr:r o:fiairoi':rrradar (comptementary,;. - 3 a.
sequence.) rA 1 till'l ; [01 s0.] n! tt 0 Y] fl 0.i nlTQ 3 9lT1 A
- Y a uX doo.t uu o.: $u [t0a 3ilQ't u?ull0 u14T0 [1J1.{fl?1.[1a.]
cA . e a attnnl,lnn?11nlultn?ltglu.]n.1ilfr1u q t0u ta 0ilgg
q%a X- Xuu.n1T tg15'n1Tfl T?0ilnfl1il1TnqT?Ail]J [T0 lO (Barry
.tr!ttnrnilr, 1 993) rrJ0riliuurylu!n:.Jn1:r1r{1rtxoJcraaaa-Y'-A.l ,X-s.l nl 1{1 f1 UA AUlJ Ufl 9t 7t1 um0 !]J ttr?',] tt0 0.1 n n1?
I.' & e t dd r a ^ XllJlltta NuLaLLunnLJtu lu?fr oT.t n1Tt1l'tttIo
e e & o eaaa Ya 3 n a,t 0't fl u tt a a1 u? u tJ't n $ n ! u.J il t? q 0 u tyt'l uuA . vv' & X6 r o ; ar lr :n yir nr :i fi o d'u'[o]' :J : : n o u ri'u i fi o fi ril u
XA A AT[t0iltaTiltqutot'] tiara0,:a1flu:lo0uilnlu
:J:rnr:tul.lr?tp r6rIor d.:yirtri'nr:um;r6'a rfl uYl tU e X C ^ e etll [OU1n?U [u01i'iQ1TIU1tfi 9tt{aq{nn'}?1J1,3au
iinr:qr:roifio#ssi'runr:l.i'ryrnfin fi 6 01{,ed<fYdMirrnlud rdu ro ln:rL riurirosfi n?1il'hlu
l, en'^):slrxofr ri oil1i'l.r fl.t ttqi fi fi :r t.:rur o,t Liebana
$
ttn;nil! ( 1 995) neirrfi.:nrral'[':1unr:qr:':o! ,.J d e X a
1iloqu?'t u.1Ft.tEt10 u [il a t?1uLnun1T[l^t'];[ga n a{:J:srurnr 71 .4o/o rrqifi.:n:;riufiq1il Rlrar?a
v e aaXaqw & a d,r Y? llofl uo? u?5.iln tTr? n1 flu ty{ u.t 2 4 g? [tJ.: [yt't1,[u
fisrrur:n:ru{'mildn1rflma'ld' (aany rrdsnrur,
1 ee3)
fi:ru,:ruro.: Lebrun [[n:flilr (1 992)
neirzirnr:tdd r5u ro In:r rrrLlniril6i.r;{AAa6
(nonradioactive) s.Jtiluttuu tfleJniltudtnilai
(chemiluminescent) o!d?utfi ilnx1ilii'rtil'rsung
nrr ll'lrlunr:o11a fi1t6'o'toi1n #rd u.: 1 ao Eo
orir.:'l:fiorr: n1:a:yr:"r fi 6 ori tu*loi'zunairrioy - yAa , d oq0.181fl ur.lnil]JT;fl!n1TIu n?1ilT'tu'ttlj $nusu
n':'r il n;16 uorou n autunrr:J fr:rG 6 nn"tnr:vrtr
fi 6 or j f.: sr m:n tfi o zu nr': n rfi o'[ri'ri": u (co usi ns.edAtAt
$fl rn ilr, 1 I I 2 ) vr':u8J fl1 tfi otJ101 n n'rrill{til 01.{
i c q & ,^-^ t
-lJ0.: [10 011til1ilo 0l.lil0u q{lu o? fi?! nil 9'] 0 n!(negative control) '[rjsrur:nuunuutd r6u ro fi, & ue A
ilutil0utJ't ifi Inro?rilfior,ln1qmtit?tnuo {.]
ruri.:aonlqi' 3 :J:snr: 6o nr:yir fi 6 arf r.rnruA vl , al Y - v 6e
:0il tnu t! 0 tuiln il l![1ju0 0unlTau? a.: [v{:ril 0:nui{dd A i ^ u, aoraura d'rutou? (annealing)' o'r[nu tl.J 14T0
adXAeun10!tuR10.lo tail t0 1a{tto110:?0nn1un:J
fi 6 or5'[n:uro{ (RCR primer)
n'l?et??o unilil'l-au rfl ailraulurdasr1nnfuTrnnfrn d la ra
rLnil rn - auio ofihou rijusr:fi fi ruIr ld'uri^&i,;,,A-r{nalilryr oElou flua.: a oRr uil nn:Jo o tffrrirlrlu
ir,:nr r nitri'r:r sr ur:nf i'r.rffnnr:dfl :roHre^dti,
n 1 T fr :', t.1 ll n il ]J'l - 0 u [ 9t 0 ]l : 0 u [ 14 0 q 0 ! fl 1.{ 0 .] 9l 0
fitrla{firRu lfi fi 6 (tubercutin purified protein
' i i q t dd f ^derivative, PPD) ?1tt0 Nilln[unnLiillt tula
o J uo r &'fl:'1.:?u t9l [ouluoroil'Lun1:n:']014'ttrn]Jil't -Ad,dati0urq0il:0u ilT3nail9l1unlTtnLt6iaqa'tn [flfr
'riluriunoufi:Jeiautd'finr:o''rrYu:ru'jr.r 6rduro murdu?n:ufiru'[n:uioi (primer) d.:rflulofilnfir-dTvleAV--N
ndIo'[moiarutdurturqdu .] nlTd!nilfr astilunlralJnun:.::.tTt'lr*tLflriflil (complementary base)A ^ & uyA . &qt{ tn 01il to uJ 0 0 tuil n il1 0{ a t0uta fl qn.:aln1ilEl 0utlTn
c{
KKU. Vet. J. Vol. e No.z July - December 19e6
<, v r ^
Aa '
4,frJ fr u?'t tilu1 tul:fl tn 00r tnu t.J1q 0.0 [fl &-tT! 0 J nu
nrr ufi { ei'?fl o r rfi o o dr rir utu qjtd rarJ r3u ( hepari n )Y
" d e a da ^
d da01 lr{1{1{'r [d 0 f.l o J n fl 1 1 il1 tq il n? r! 8i9t1 a u y,t l"t 0l
rud'rur'[r.]r-jr (incubate) fioruunfi 37 onfl1-
tsf,16um rflurrnr 1o-32 d'rir,: ruii'rrirrfioqd-":urutaSotrn'rtriluuunl,lo-reilt (plasma) noratr
Jvt- .n,,r L9tO:U't lil fr n']:q:'la?uoiluttn il il't - 0 utq afl T 0ug
Io ul"i'rnerfro o''[a io oia'[rJ (wooo uo:rlru!, 1 990)
fi:ru.:ruro.r Rothel rrnrnrur ( 1 990),a.iuJ
n il'r1 n{ r{ au Lr?1 o ;yir1r{fi nr:air.: $n il }J'r -AA0!ttq0yl:auiltnrfiq n0 o0.ttnut00oLufltr
9
fl or nlunr:u6.:oirt or rd oe 6 a rarlrSu qia.::-i rrfi oqnrulu a d'rir.r un-.:arnnr:rfirrfi ooornln
rAaddn0u111nl;!tJtfla9tqto.ttoilfl.lT]1 1i 9t n0u fl1'tu
rrirduro.rrl' fi 6 adr.:rioun-t:1d' zo 'lruln:niruzA . da a a a Aq a d a A - Xtnoo 1 {T 1.,,1 1l o rttflt:tilult}\ o ?tf,Tt,latntrout f a dt ,
NU LfrlLLU€tnLlU3J l,1J )fl ::U:tldtLUntTL}J1-t0UA a - r -; q ,
Yrfl9lf]0 1 6 T? tll.i rtil:o{uunlJylLlt}Jt:fruLunt:LsJ9t{
6o gz o.:nrrqnrdua airuiu:l3lrrurfi oofirir:r'r
rirueio.:rurnn-ir 1.5 6fi lfianrrrirutunr:urntnL y,l d't fr tJ't n'l:tn L tn 0 q Ql n fi q??1 9.1't u rLn1 Q3tl
n n virtfinr : air I un I I r - i u rer o fil : o u ul 6 u u url n.:vl r - E vt , o d * (Atu 9tJUULiln?:Uttn0qalnflq?nfltutLd?8J'tqT? AfrEL
1eo.1 $ilnlTar?0141lrns.J]tl -aura afil:0il a:
fl'r tJ r :n e :'r ai fi o d'ufrxl: fl 1(i' uoi zu n fi 'loi'fr fi n rr ru
tuj:rj:r utri'rriuri'ule u fi a'l r14 o rJ 1 ar n n r :il urdouIoi rfiorrdaouoltulrnrarur :gu.ir.Jliuoo n-lr
tfi u uunyrnrarr uiofi nr:roiruro.rrrunfi 6uun:l n :rtuEr-r o ri1.l 16 a or ruryir nr :ii l rra r n-lr q :? a.e^ - J-? uo q u u u.t r n o il fl n: u't n ! rlo n Tll - 6 U r o o rnlr o u
(Rtpna-tnterteron) un;n:si'r6ureofil:ou (Beta-. \U!,4 v - Y A y A ^ Nrnreneron) to anq? u onuutl40nq!qil1?tartnotil
4-\,ea a Yt na.: tolsJnlTult01tytfliln a [a ro trtrtrt.:ulunr:
v e A qvoqT? Q?U0 0uo? u [y1 0 t?i il nllria1 r]t13Ei O un il ]J-l -AdrAout9lailT0illJ1n$il (Hothet unsnrr{3, 1990)
65
jtiq.,dadaavua rtal nil tFt?'r t14 t{ R n L 0 0 n't5ilqn't tL 8:n-l dl-ttlJ]q a d r ^ iq[u2?14l{J ou0'rotJf,tn4o]J'totn lfloqtto Lur;u!
r[Tn,] ui0 ril ul:n rr:-r rri': jr.r n'r u ( general ized ) fiqo^d - X A a v a
f,'ttJt:n tT1DgtT?O1iloiluu tlt 0ul{uunJntTtno
{rul:ntulnr'loi (tteitr udrn{u:, 1994) ehuira d v iqq
t?lFluft 0 La t0 Yr [tlunlTErTl0 itnilil1-0ulq0-I o-
fil:ou riu['i'uLL $ruir 6'loro (sanowich EtA)
Iouur unlJil'r-6utqafl:au ornlnfi6orfr'aqf t a d qr d ,
xulauunntiuu lulfr tJrto[fuutilla (mouse)
rfi otrisirlhluln n ua o ua uAL o 6 ( monoctonat
antibody) qia unrr-r-iurqafl:au riu ufr:ur
ruou6! o 6 lr yi'r rJ fr n3ur rir oir a dr.: u nr arr-&^ii(plasma) un.lalnuufl'l.l00n tail[t0UqL09]m
t <Aaoilnrn (lebel) olutau L{:.J6x.30:1,r'rtJfl n:urnLY - o o uA - Afrl:4.:ou (substrate) nr tuInofl ry.JRleJ']tn
eiruzuntd"ir o-?arirJy{nrnrrriu fi unrrr-6uroofl:ou odo3l (notnet un:flrr:, 1 990)
oriri'l:fiqrril fi:rl.:rur:0.: Rothel uc:
rril: (1 992) neilrfr.rnr:q:?a unril't-q d I qt a d u
autq01,'l:auturn09 tqu LTtrflun 0 tel ta tlfilrr:nrff qhR!?n rfi a un:zunnr rfio'lqi Hrnyirnr:
u d a da a eq?1 0 R 0 1J 14 fl J n'r T il O ?1 1 t 1J I : O I il U tlj't I U r.] I 14 U J U A U
* - Y ue. da an?1 59 1u oJuu uln torlnlraoyjrtLai€rr6tuv\ a uU, e
tljr [ur.]114U.t [1Jttn? n?:t't1-[T:UsOUlJUOU 59 1U
rio uo yyfr nrrfl:'lai fio {i'uqir u nr:n:r Q un il ilr -6 u r sr o fl : a u ri a u ? n rfi o e-J n ai r r r fi o du'[ d'r fl o.r o r ni - r dd - i d , :,
tT0uU Lfl ULFtytt:utug 0u u5oornnr::Jilt-:Joilj
' , A
' q eaI 0.i fr .t ttil n n :l R 0 tJ yl R1 il'l : n n :: qr u lvit n o n I T fr 5'] J
UttLn il lJ'r - autq 01lT0u iq [tnrgJn RU tyla o']atnqorn
,&;,'rnlTLJUtil AU1 0J fl.t rriln nil n ouy]fl't tJ1Tnno fi5a- c d, X*^uu il.t nl:s:'t.t unil il't - Suln 0y{:0 u ilo na 1 nu u.J u
T'tu.l'1111J0.3 Jones riRsnil; (1 992) nsirrfi.l
nlTn q:J{UH't r.J n:Jt n lfi0fi rfi oor n ran rr ol:filn
rrouGrod (heterophil antibody), Tilraoud-q
trvlntqai (rheumatoid facter) Tounr:r6ru uofntlrd 6it (normal mouse serum) dlorriru
66
P1 - -'v-.,, - ja a t f a d
il0.rniln1To:J11r.,t01{n'19?0.10ilil tulnnu6u n
(tsc)'[qi
n1 ?ilet 6l oil n n il'l5 a a dtari(u
nrrYr erffou n nvrr:o n 6'[arj' rflu6 n nr:- A A oo s Iqilai fra fluufr.:fr nrerr:n'L'fr'q:ro soulnfi riJu
iruT:n'[ ai' I o r n r : r fi r r fr a o o r n T n oi'r d a.r #u'i rriluinrl:n rurp{aeln-:..r 1 .25o/o ?0.:fl1:n3fl1u
dlt < A ^ ,J , An nYr1T0 no taq fi.:03tno n1:rilfi uuttiln{1 0.: rn ooq
q! t ^ d.tnoLilunnuils t0nfliltfltll tilarilai (gelificationA a r i, ^ qe a epolymer) {{rn0oa'tn tnytilnqt0!tltr?n1 liln1:rnq
Jt^(tonhfllrnru [nfrrruoi et'']nn'i1 9 il1fi uqid'rlf
t1 n1#o un'j'l 4 urfi rird'ir Tn q"'rrful'enr l ifi u.:
qianr:riluirxl:na.: (xanter t[d;rtrur, 1 993):ilona1nfi Kanter $nrnilr (1993) u.ln6imfi.:,Et,4 X' dt ^a i,lJ 00? 0{ n1T?10 flauil?'t ril u?D n1:yto flolJ71.t1u
r? o r;? T1 nthi utnt.J,'hi ri'0.: yir nr :yr o n a.:#r rd oa v , q or i<, u su u u u r.t d T1 u Liln1 T rr u n x u0 a u I n yr til u,l r* [:n tt:t :t
ruo q?lud (advanced)'ld' uns nr rur:nrir'l:Jtlilu
n'ln fllr'r il'ldTo u rililtrtilyi'o,: fi fi ar'nt::;u'] olJ o.JIrn
rqi'lil fi d's.l il fr ri6 nr::a,:i:t o rir{'l:fi qr ilaifi fi fiv a a u | -T i<, f clJ0rfrrjrgunil n0 t3Jfr'}8J1:n?UOflUln?itilUt:nLil
s a i<, t ATsu3oil 1 14:0ytrlj11tTn[r:l]Jlfllt1;n (local) rrn:q ur Jf
fi 'r,J'r r n r fi o il fr n3 u r'[u n r : m q fl a u'[ ei'[ o u fi [ n'[ ilrfl uI:n ur nIn q"'r #ufi nnr n s o.: nr :ii'n r ru r{o i,r
(chronic inf lammation ) airurrmr:'[ru0rn']'1ildl r]1.13
Kanter ttntnilr ( 1 993) :1u.t.tui.t n.]rfl:.]ove^oJ^M
r froauri'r lifrfr fr n':r ru'lr 85. 4 o/o ttnyn?t ilqi1 [1 11 g
97 .1qo
9)4
n1r[il'lv[da
X a v u, d, a6 e o Yn"lT[y{1; rT0n 0 lfl ']'t tlunl:iu0fl u h,[11.[-Aaa,r-Ya^A0ailfroyt1u?105u1-[rJU"]'t too'tuulLnrTqtqrfra
xlra{1:frn?[r?{flrJr{1a{a{ ils. flfi o or;ufi 2 nrnn-lail - fruarnru 2:c}g
r '.
d4 t a aNiltn[Uflnnilil lU?fr t.J Koneman ttntnily( 1 9 8 s )'[d':r s.: r urd r 1 n-:l n 1r e r? o i fi o d'u':-rul:srf { I' T .i d vt0EJn1Ttltl3tr0 t? toutTilalnnl:tnLoloti1.t
, iq -, u e , ? ufl{oT?1 0?aul.ln[Tfl.:9lTla totln u1 hJfluun.3y X A - i ,d a Xu fr fl't?c rua ru 01 a{ a't u?;rn1011 iln1:qto tto rtuovla,Xa<teAdqil tlnTyon uTaqoilu'tt14d0.l tlilqtu,iitnr:triu!
.e6eXla't0u'].Jfl.:aT10 0a{m10?utrnilniln0qtT0 [1r,10
o o rdo 6 ufi o r:J u rf, o urur n-u oir o dr.: ai.: qr :r o tuTnjq., A a t Y a
o? I u't,l?1 iTfl.l [y{lr !10 n 0 9t 0 lJlt'l tlt fl 0{ fi n.]al n
riurir ql-? o d r.r eir Er :': o'[:J vrh lvi:J l a o r n n r ::J u ri] o u
(decontami nation ) mrfi td'tfi o yrhtd'il :r Fror n nr:t 4 , q .€r a Aq qe dIJUtU0u fl?ulfitutljufllTtfllJ {.]LUnr:lrjsr:ren
. .: o - at6t.Jnn'l? q 0.1€t't11.tni ailaT1u01n m:tnilq 0nl:ilda A s irfi i qr: a or or rfi a 1 o uri'r t:J ruurri'rtri'ld'Lsr:Itrd u ru
r4oarvlq (trisodium phosphate) ilflilfi'ilr!u{anfdudtntuUil nna tTfi (benzalkonium chloride)
<, aAa$^t r"t ; rij il fi 't rr n il- fi fi o r r el :u rr:l ria u ( m i I d
decontam ination ) uiof i' z,uo Ttr 6 u ru'laqro n'lfi (i'
(sodium hydroxide) zuslri'lrdu-ro-uod-6(ru-Rcetyt-L-Cystiene, NALC) ril:liriJilfllrrnfiAa e d ,;fr fr o : r I :u u:.1 rio rL u n : ff nr : r :J fi u u u:J n.: el 1't rJ r fl u
tadeAn:q 6i'r{ rfr u.i rfr n{o u r fi o 16 ru mr:n s nr u yl o srrnlq -r.t-r,'l n l oi (phosphate buffer solution ) u n-lornnhlsr X dtfi:JT'r fl01n tta ufl?u1lJ',t t9til R'tTflrn1u:til ril a5
ni:r A il flrrn y R''l u iJ-14 ril o ifi o e il:y s.i rirfi o #r.:tAqrrodr.:6i.lq:ra t6oorlar:fi u ltnsnont]ilri?.J
qir rflrrr a{ ei'r o drr ei.: q:rn rfi a n.:r ruir ulunr:L:Juuunflrnau otnntrdnr*roo:tnr:ri.:ro.:
"!snr:ilullun[qu Koneman [[nrn.u; (19aS) nliri .q. J
0 q :1 tT{ y] 0 r ?1'} t14 ht d 1 a,l n 1 Ta T? a y'i ! lfr 0 o 1 n n ] TXndn:roriltfioilufl'[nci (smear) unrnlrrlitl;rdafi
, a A-eir q.: ao 6 ofi o,q:r ri.t 3, 8 o 0 ryirr a.: u:rT#lrit.lflt
r v Au-I o.i I n n ra n''.: or n fi '[ri'el tn o uet n n']:fl u uu n rur rrit''r
il1 A r n 0 ilo.t n d't ? il'') ty,t'l I IT 0 [u 0 1 14't T r fl u { rfr oX:Jq-:vr
0'r 14'l T r n u.J t t 0 yt tt r il r y r T o il u I o tr lJ et yr 15 u
atr ulrarlrJ::n oti ei'? u'h.i 1*note eggs ) zu{ siur.li.l
KKU. Vet. J. Vol. e No.z July - December 1996
(potato powder) nfirto:on rrairrfio uqi euinn
ud:flm; (t 994) uu:urtu1forr.,t,fr'ur16'.fut-tf, t'tufi Lqtu-t0ut{u (Lowenstein-Jansen media)aJXr
u5o a rul:rfi t.: 18o alqul3.r ni (Stonebrinks media).e j .l x XttJ 0 11 ua:n 0ud.J tu0'tut:tn uJ tto Lr6i? ul or o ril.:
Ju Atwr:rfro'[ririrfr orur.rnfi 37 a.:fllrsn16ua firi'r.rIU
nriu au'[o o o nlryqi- g - 1 1,u, d.r rijuflnrildru ilrI^ i &T r nttnnl:taTr_u"IJ0JtTa &ulnttuantiau luTm nl:llL
l'fi'rr or r-l :, ur ilI z 4 - 4 o fu u n-.r o r n fi 'ld'vir nr :ri rX aX ^ XlT0ttotltttot0Til1Ju toru]lJ1yt9floLnilf,il1J6
d a , -
- i -'-
fl.l{fixrflfi rfiouunrfletlo.t16o (typing) d.t
n .t : yr q fi o :r n ru s ru ri6 r r.r 6 r r o fi r fi o t u*l d'zu n fi u ri
uauo:tdrrnril::llrru 2-3 frlqrf (Liebana
rrfl:flrtr:, ,1995)
o rir.:'l:fi el rJ nr :rnr: r6'a1r.r"lqizu o 6ducio.:si'tul n.::loo-uu nt lrJ:trrr: rdu nt:ilurfl au
iJ .t v d 4 &1to.rrlu0o1.{ 1 tuor0u-}J6i.:orto uTa LurJuoau
rj fr lGl r u nr :tii'or : ro fi lunr :yirtrirl :r fl ar I n r r., .l a y d o q ea ;Ul-[[U 0l,l .]t.:L tn 6t:n a1 o lt zu nyr-r tdrfr ofr ei outta ql u
nr"lqi eo-goo,, (euinn uRrflru:. ,1994) drritu6t
'^ € u e d Y XhN tu tno h R n u tra Lo I t?lJ n1:[1^11: tfr O O]'0.3 O.tfi U
:r u:r? n'rfiFiourir.:urutunl:qr:r aifio d'u rioufio:l'rr-t Lt e..t d In oI o fi nr :yrioir url a IIrfl '|il lJI n $ #.)
;^trilafr or:r*r [ol:r:Luai'r a:rfi uir nr:fia
fi r t u a ifi r fi u rrirlu fr r u rir riu r H rL -,
= fi r
= ullill"i'
t;[unrn surru rfi o.:ar n atLr:nrJfr l-Gld'ir u fi nl eL
rir 16 a fi a n a al o r : rirt'i'qir u'[ri a.: sr rLr :n r.l rl.].r r-rgJzunrd'{orndo 72 d'rlu.l udinl:rJfrf6'lilci'al
or d'u:J:c at.l nr:n[rur n fn uqinr:sr:': oi fio d'u
r-nrT:n qi": unr:fiofit rl affi-:fi urdrlufitr.r ri.: fifl1 eJ1:n r fi q zu R u x n rfi a u n r hr n n L rfi a'[d' r.r r n'l :.io4cv-l
n1u.: n{!00 uyl 6i{ zu n n:iyt! eiontrqrroifio d'u
nr:'l'f'rvrofin fi 6 or{ irrun-:.r 6 riu ro In:nrfl r-uyr n fi n fi fi n l ru'lr ri o urir.r st d.r ]J..]:n :1 u.n u{l.r a'[<i'nr utu 2 4 r%Iil { usi oi'a.l or s-u rifi fi n.lr ru{rfrurq uo::J::s:rn1:nirlln dnyijsirtfsjr u
l-tdAaat n u r n L 0 | n : il Lr R r I Ft T 0.3 il o n o u{l I n :r n r tt}^,t.:
Ixuonotnu u.tfr']lnTntnor.lau?nrrQ un;zunolrno
1 ei' 14'l n 1 r gr n'r'r rL : y l-o : : i.: 1 u : : u'i r .r riu o o u
rJ fr:rG.:ru nrra:ta unlJ tr - 6uro ofil:outurfi ooT 4w a d u ! otou !rtnfluFt 0 L0 ro triuonlturrl.:r.rfr.: [unl:
r ioo T ? i 1 u a il u ) r* [ : Fl 11 8J n ? I 3.J A1 [ 1\ 1 r n A UlJ 1.] R.t
qi r td'qi r u'[ ri ur^r.: r r n rin Rl il r : n :r u.r r u zu n'l oi.l
^ e ,.1nlu Lu 2 tu t[q tu1l{u:tqu?nunfl]ill:ntnohfl
ll n rfi o trn : zu n n r rfi o'[ri' 14] ntr q fl 1r ]JT o L n rl u
run;nlr l:;Lr-o::r-.:lul eu:rJ fr 1-6 ui01il filfiJ fi.re - ia ,Laau?1tlr"tdoantTqtTla?ilailu nl:?10f,8]J
dr4 d < <t d ad A Annyt'tT0flq tagl ntilu0n15uu.:.It.:o'ttJl:nn:ro
ifiofi:-nrl:nlnl<i rlfr lGiru :rer5r un:rirld&
sir u'|il uili uqi fi rff o nr :fi e il n1 olunr:qr :ra'[d'rar ne dA - X Af,fl1tnonll:r]tTantRLLTO:.J atnfl-itilflorr.: 1 61J.:
u,-ievsoro'Lrirfrl :ril Iru[:o drunr:rw-r:r6oriuriJug . iqq :. X - Y -. -"'
ri u q o u m o yi-r I fr Lf Lu n I : ri,r 6 i r r fr o o-r riu r il u r 6 of a! iq. X5rn tJ n yt LoQ1 n nI: llnt I : tt a o o udr,: t$iuo1{ ttsi nr :
: - uL J;"tl\'tytta n €lta tno nlt lJ h o1^l n te Lo u r n tt oytur :LrX
Ll n: tn u.J ela'lu1 u1.{0 u LLnI 0 Il.tLLO tt5o oto tn o nl:, -Y i.i u-
rJ ur:-l o ul o.: rt a du'tq{ir lur n firJ fr:tGtr o Fnr er
x: : r o : ; T.: u a n o 1 n fr n 1 : r n l ; r 6 a u-.: qio.r l'f'r -l
n -r
qJulu Lun I::I U.: IUh n nr:tllr:tto trn:n rualr.J odd
rlJT? [ftlJ
d5ilq
orrfiu'lei-j.) uurm.:qir.:,1'lunr:o:.:oev t t , v d e - y&
?uailu? ru[Tn ta 01.t n tJr 0qrrn:riotRuoir u nuyr.luu..-'.4 o d d <r-.rJ lvl r! . q
',a eaI o [ il 0 n 1 u.] n { Fi ?'t lJ r il u'L il'[ o 7r o y ur ru r t "i'r.J fr I o
,ian?rsJti']rfraff a nxleJdly{trud;n?1ilh uns:ru;..1r'tR1 tunlTor?o?ilailuIn? riro:urlfnr:or.]arun eJ rn - auret ofll:outurd o olo r.rld'ryr nfi n 6'la r o
ir ru n-r nr:dq fi r rL o f6 r fi u rririufi r u ri.r r rlf.^4- & ^ oo d ^-.I A,ruo.r0'tnm.J 2 1r. ltft?-1il'[rtrRso?rsJsirrnl:frilr
tfr'offo srrurrflT'rrJ,t'ruzufltri'nrulu s {u unski
67
68 ?1ra{'lrfio?rnryruc{1a{o{ il?J. iJd o au-ufi 2 nrnglarJ - #nercrru 2539
12 e eAa t d o
9r 0'3 0'l fl u tl ?1 il ! Ts fl ! n l t nitr n r fl 1.1 rJ t.'l u,t il il 1 n!va Y A " a 4 , e e, I o ua nm{ til 0 fi1 u.l n{ n1 Lt0't uyt0s [tr iunlTqr? oeA v e & ^ ^aX, A r'lu0quttn? ?1.t 2 ?r'uillo!r14tjl;flil?1flo [qUO:
Idr{4tA
il'r n']Tn:l a ttntJ il't - 0 utfl ay{ T 0 il hlt6i 0 q ri ail n o
d 4 t yA . 4 a da a[n u t a a 9] il"] n a u rtn ? o.t yt't n'tTfl oyt? u.J oin? d ur{.)q^-&tuil?14U.t 01nUU:JT;tiluunnlTaT?a1froau Iou
01#u zu dalnnlrqr?aifiod'uvil z ifr:l:sn oufi'u
:Jiurriuf ooil:Jrsrfl?o. 2s37. T:nfifinrrruairri'rytur4r{lrlnuilunynl:n?uqruflo.:niu tu : nr:d'onr:aJnt r un; H J^A rtur t'.rfulnur:. I:.:fi ruy{sr:rur nru. nr{ ryryle : 141i.r 6 8 - 6 9.
Barry, T.,Glennon, M.,Smith, T. and Gannon, F. 1993. Detection of Mycobacterium bovis in bovine
blood by combined PCR and DNA probe methods. Vet. Rec. 132 : 66-67.Cousins, D.,Wilton, S.,Francis, B. and Gow, B. 1992. Use of Polymerase Chain Reaction for rapid
diagnosis of tuberculosis. J.Clin.Microbiot. 30(1) : 255-258.Francis, J.,Ohoi, C" and Frost, A. 1 973. The diagnosis of tuberculosis in cattle with special reference
to bovine PPD tuberculin. Aus.Vet.J. 49 : 246-ZSi.Jones, L",Cox, J.,Shepherd, J. and Rothel, J.,Wood, p., and Radford, A. 1gg2. Removat of
false-positive reaction from plasma in an enzyme immunoassay for bovine interferon-
gamma. J.lmmuno. Methods. i53 : 233-240.Kanter, l.,Lopez, B.,Torres, P.,Nader, A. and Garcia, V. 1 993. Preliminary evaluation of a simple
method for detection of bovine tuberculosis : the glutaraldehyde test. J.Vet.Med. 40 :
27 -30.Koneman, E.,Allen, D.,Dowell, V. and Sommers, H. 1983. Mycobacteria in : Color Alas and
Textbook of Diagnosis Microbiology. Koneman, E. 2nd Ed. J.B.Lippincott Company :
pp. 459-505.
Lebrun, L.,Espinase, F.,Poveda, J. and Vincent-Levy-Frebault, v. 1gg2. Evaluation of
nonradioactive DNA probes for identiflcation of Mycobacteria. J.Clin.Microbiol.3O(9):2476-2478"
Liebana, E.,Aranaz, A.,Mateos, A.,vilafranca, M.,Gomez-Mampaso, E.,Tercero, J.,Alemany,
J.,suarez, G.,Domingo, M. and Dominguez, L" 'l 995. simple and rapid detection of
Mycobacterium tuberculosts complex organisms in bovine tissue sample by pCR. J.Clin.
Microbiol. 33(1) : 33-36.Neill, s",oassidy, J.,Hanna, J.,MacKe, p.,pollock, J.,clement, A.,walton, E. and Bryson, D. 1 994.
Detection ol Mycobacterium bovis infection in skin test-negative cattle with an assay for
bovine interferon-gamma. Vet.Rec. 135(6) : 134-135.
KKU. Vet. J. Vol. 6 No.2 July - December 1ee6 69
Quinn, P''Carter' M',Markey' B. and Carter, G. 1994. Mycobacterium species in : clinicalVeterinary Microbiorogy. euinn, p.Mosby-yearBook Europe Limited.: pp.r56_16g.
Radunz' B' and Lepper, A. 1995- suppression of skin reactivity to bovine tuberculin in repeattests. Aus.Vet.J. 62(6) : 191_194.
Rothel, JS.,Jones, SL.,corner, LA.,cox, JC. and Wood, pR. 1990. A sandwich enzymeimmunoassay for bovine interferon-gamma and rts use for the detection of tuberculosis incattle. Aus.Vet.J. 67(a) : j34_137.
Rothel, JS.,Jones, SL.,corner, LA. cox,JC. and wood, pF. 1g92. The gamma-interferon assayfor diagnosis of bovine tuberculosis in cattle : conditions affecting the production ofgamma-interferon in whore brood curture. Aus.Vet.J.69(1) :1-4.
wood' P',corner,L and Plackett, P' 1990. Development of a simple, rapid in vitro cellular assayfor bovine tuberculosis based on the production of gamma-interferon. Fles.Vet.Sci. 49 :
46-49.