proyecto de investigación del año academico 2015

8/18/2019 Proyecto de Investigación Del Año Academico 2015

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Universidad Nacional Federico Villarreal

PROYECTO DE INVESTIGACIÓN DEL AÑO ACADEMICO 2015

EFECTO HIPOGLUCEMIANTE DEL SINERGISMO DE PHYLASIS

PERUVIA (AGUAYMANTO) Y ANNONA MURICATA L. (GUANÁBANA) O DE

PHYLASIS PERUVIA (AGUAYMANTO) ASOCIADO A SMALLANTHUS

SONCHIFOLIUS (YACÓN) EN RELACION A LA GLIBENCLAMIDA EN

RATAS INDUCIDAS A DIABETES POR ALOANO

HYPOGLYCEMIC EFFECT OF SYNERGISM OF PERU!PHYLASIS(AGUAYMANTO) AND ANNONA MURICATA L. (SOURSOP) PERU!

PHYLASIS OR (AGUAYMANTO) ASSOCIATED YACÓN (YACON) RELATIVE

TO GLIBENCLAMIDE IN RATS INDUCED TO DIABETES ALLOAN

A"#$%&' B$*+$ C,%,*-$L*/, B"'#**#& R$3*' C&'*% 1 C*4&%$ M&$

S&*'#,* 1 E'-"4&%$ M&3,* 67$1 6**-* C$%',$6",$%1

(1) E'#"4,*#& 4& * F*-"#*4 4& M&4,-,* 8H,9$,#$ U*"&:

Facultad de Medicina “Hipolito Unanue”


2/18

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1. T;TULO

EFECTO HIPOGLUCEMIANTE DEL SINERGISMO DE PHYLASISPERUVIA (AGUAYMANTO) Y ANNONA MURICATA L. (GUANÁBANA)

O DE PHYLASIS PERUVIA (AGUAYMANTO) ASOCIADO A

SMALLANTHUS SONCHIFOLIUS (YACÓN) EN RELACION A LA

GLIBENCLAMIDA EN RATAS INDUCIDAS A DIABETES POR ALOXANO

EN EL BIOTERIO DE LA UNIVERSIDAD FEDERICO VILLARREAL EN

EL 2014.

2. FECHAS F!"# $ I%&!&' 1 $ *%&' $+ 201, F!"# $ C*+-&%#!&% 2/ $ *%&' $+ 201,

- $ $#$< !# H'+3@-#%% !'% *% ' '-$&'

(210107) !&9&'% #7*# = #+&-%3' # +&93#$< !'% *% &'$' $

#!+&-#3#!&% $ &3 $# = 8*'% #%$'-&@#$' = $&&$&$' % & 7*'

(%'-#+< !'%3'+ #+'6#%'< 3 $'& $+ 63#!3' (200< 400 = ?00-77) =

7+&9%!+#-&$# , -77< $-%3 $*#%3 , $# M:3'$' +# $3 8*



3/18

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&%$*!&$# % #3# ' &%=!!&% &%3#&3'%#+ $ #+'6#%' -'%'"&$#3#$' #

$'& $ 1>0-77 # 3'$' +' #%&-#+ 6!3' #+ 7*' %'-#+. D*:

$ 24 "'# +' #%&-#+ * %3#'% *% %&+ $ 7+*!'# #%7*%#

2,0-7$L 8*'% *#$# ## + 3*$&'< +' %&+ $ 7+*!'# % #%7

8*'% $3-&%#$' *#%$' *% 7+*!-3' +!3%&!' (O3&*-) = -&$&

+' %&+ $ &%*+&%# -$%3 + -:3'$' $ S;%$&!" 7% G'%@;+ =

!'+. T#-9&:% #+&@ + 3*$&' "&3'#3'+7&!' $ ;%!#< "7#$' =

&J%. Re$!ta%& #+ #$-&%&3# 200-77 $+ 63#!3' 3#%+&!'

'9 -#=' 8!3' "&'7+&!-%3 ( -%' * 0.0,) = -'

'3!!&% $+ ;%!#< "7#$' = &J% $+ $#J' !#*#$' ' #+'6#%' 7%

+ 3*$&' "&3'#3'+7&!'. L' "#++#@7' '&9+-%3 *3&8&!#% '* +'

#+!#+'&$ '$#% &%$*!& !!&% $ &%*+&%# (G#!# L@ 3 #+.< 2004)

+' 8+#'%'&$ +&9 #!3*##% !'-' 9#$' $ !& #!3&# $

'6&7%' (V&%'% 3 #+.< 1K/,)< = +' !'-*3' 8%+&!' * 3##%

+&7#$' # !3' '+&8#$' $ '6&'-# (S&# 3 #+.< 200,).

C&'!$'# S -'3 -#=' 8&!#! "&'7+&!-%3 #+ *3&+&@# + 63#!3'

3#%+&!' $ "'# $ A%%'%# -*&!#3# L. (G*#%;9#%#) #$-&%&3#$' ' #

'#+ $*#%3 , $# % #3# $:3&!#< &%$' +# $'& $ 200-77 +# -;

8&!#@ !'-' "&'7+&!-%3.

PALABRAS CLAVE A''&'a* e+trat& ,e-eta!e* %aete /e!!t$ t0&

1* -!e'!a/%a* -!$e/a.

TITULO E+&-#$ 7,9$>,-&,*#& -$*4/"?*#& 4& &@#%*-#$ *,-$

4& 7$3*' 4& A$* "%,-*#* L (>"***) & 9*-,&#&' -$ 4,*&'

#,9$ 2 *3$ #%*#*,&#$ 4& >,&-*,4*



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RESUMEN

O2et, D3-&%# +# 8&!#! = 7*&$#$ $ !;*+# $ 63#!3'

3#%+&!' $ "'# $ A%%'%# -*&!#3# L (7*#%;9#%#) -; 7+&9%!+#-&$#

## *% -' !'%3'+ $ +' %&+ 7+&!- !'-##$' !'% +#

#$-&%&3#!&% $ 7+&9%!+#-&$# '+#< % #!&%3 !'% $3 -++&3*

3&' 2 . De3 E3*$&' !+%&!'< #+#3'&'< 7*' ##++'< $'9+ !&7'.

L$-ar# S&!&' $ M$&!&%# I%3%#< H'&3#+ I ES#+*$< !&*$#$ $ T&%7'

M##< D#3#-%3' $ H*;%*!'. Pae'te# P#!&%3 !'% $%3&!' $

$3 -++&3* 3&' 2< 3#3#$' !'% 7+&9%!+#-&$# . I'ter,e'&'e E%3

-#=' = 3&-9 $ 200< ?0 #!&%3 8*'% #&7%#$' # ? 7*' $

-#%# #+#3' > 7*' !&9&'% !;*+# !'%3%&%$' 1/0 -7 $

63#!3' 3#%+&!' $ "'# $ A%%'%# -*&!#3# L CEAM -; , -7 $

7+&9%!+#-&$# = +' '3' > 7*' !'%3&%*#'% '+' !'% 7+&9%!+#-&$#.

Pr'0a!e /e%%a %e re$!ta%& E#+*#!&% $ +' %&+ $ 7+&!-.

Re$!ta%& E+ #%7' $ $#$ $ +' #!&%3 3*' %3 >/ = ,4 #J' $

$#$. S %!'%3 $&-&%*!&% $+ %&+ $ 7+&!-< &%$' -#=' + 8!3'

% #*++' * !&9&'% 7*#%;9#%# -; 7+&9%!+#-&$#. S %3

8!3' #$' % , #!&%3 $' $ ++' 8&&'% $'+' *%3 %

&7#3&' #'!$' % *%' # %;*#< = % '3' > '+' %;*#. N'

'9 #+3#!&% $ +' 6;-% $ +#9'#3'&'. C&'!$&'e# E+ *' $

+# !;*+# !'%3%&%$' 63#!3' 3#%+&!' $ A%%'%# -*&!#3# L -;

7+&9%!+#-&$# $*#%3 >0 $# '$*' *%# -#=' $&-&%*!&% $ +'

%&+ $ 7+&!- % $:3&!' 3&' 2.

PALABRAS CLAVE A''&'a* e+trat& ,e-eta!e* %aete /e!!t$ t0&

1* -!e'!a/%a* -!$e/a.



5/18

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TITULO EFECTO HIPOGLICEMIANTE COADYUVANTE DEL

EXTRACTO ETANOLICO DE HOAS DE ANNONA MURICATA L

(7*#%;9#%#) EN PACIENTES CON DIABETES TIPO 2 BAO

TRATAMIENTO DE GLIBENCLAMIDA

O93&' D3-&%# +# 8&!#! = 7*&$#$ $ !#*+# $ 63#!3'

3#%+&!' $ "'# $ A%%'%# M*&!#3# L (G*#%;9#%#) -# G+&9%!+#-&$#

## *% -' !'%3'+ $ +' %&+ 7+&!- !'-##$# !'% +#

#$-&%&3#!&% $ G+&9%!+#-&$# % #!&%3 !'% $3 -++&3* 3&' 2.

DISEO E3*$&' !+%&!'< #+#3'&'< 7&' ##++'< $'9+ !&7'. LUGAR

S&!&' $ M$&!&%# I%3%#< H'&3#+ E S#+*$< !&*$#$ $ T&%7' M##<

D#3#-%3' $ H*;%*!'. PACIENTES E%3 -#=' = 3&-9 $ 200<

?0 #!&%3 8*'% #&7%#$' # ? 7*' $ -#%# #+#3' > 7*'

!&9&'% !#*+# !'%3%&%$' 1/0-7 $ 63#!3' 3#%+&!' $ "'# $

A%%'%# M*&!## L(CEAM) -# $ , -7 $ G+&9%!+#-&$# = +' '3' >

7*' !'%3&%*#'% '+' !'% G+&9%!+#-&$#. PRINCIPALES MEDIDAS DE

RESULTADOS E#+*#!&% $ +' %'+ $ 7+&!-. RESULTADOS E+

#7' $ $#$ $ +' #!&%3 3*' %3 >/ = 4, #J' $ $#$. S

%!'%3 $&-&%*!&% $+ %&+ $ 7+&!-< &%$' -#=' + 8!3' %

#*++' * !&9&'% 7*#%;9#%# -; G+&9%!+#-&$#. S %3 8!3'

#$' % , #!&%3 $' $ ++' 8&&'% $'+' *7%3 % &7#3&'

#'!$' # %#*#< = % '3' > '+' %#*#. N' '9 #+3#!&'% $

+' 6;-% $ A%%'%# M*&!#3# -# G+&9%!+#-&$# $*#%3 >0 $#

'$*' *%# -#=' $&-&%*!&% $ +' %&+ $ 7+&!- % $:3&!' 3&'

2.

PALABRAS CLAVES A%%'%# 63#!3' 73#+ D3 -++&3* 3&'

2 G+&9%!+#-&$# 7+*!-.

TITULO EVALUACIÓN DEL EFECTO HIPOGLICEMIANTE DEL

EXTRACTO DE HOAS DE CHAPUCA O UVILLA SILVESTRE

(P"=#+& *%#). EN RATAS (R#33* %'7&!*) CON

HIPERGLICEMIA INDUCIDA



6/18

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S $#'++ *% 3*$&' 8#-#!'7%'3&!' = 8#-#!'+7&!' $ P"=#+&

P*%# (*&++# &+3) ## #+*# * 8!3' "&'7+*!-%3 !*#%$'

#$-&%&3#$' % #3# #+9&%# (R#33* %'7&!*)< $*: $ +#

&%$*!!&% $ "&7+&!- !'% 2 77 $ ' $ #+-&$% '+*9+. P## +$#'++' $ +# &%3&7#!&% *3&+&@' > 63#!3' $ P"=#+& *%#<

"!"' !'% $&'+%3 $ '+#&$#$ !!&%3 (:3 $&Q 3+&!'< #+!'"'+ 0

= #7*#)< -&-' * 8*'% #$-&%&3#$' % &%3&!*#3' #3# #+9&%# $

2K0Q ,0 7 = $&3&9*&$' #+#3'-%3 $ / +'3 $ > #3# ' 7*'<

%3#$' !'-' +'3 9+#%!'< '9+-#< '&3&' = , 3#3#-&%3'

#$-&%&3#3&' # +'3 9+#%!' %&!#-%3 + "!*+' (A7*# T% /0

(1)) +'3 '9+-#< #+-&$% 2 77 +'3 '&3&'< #+-&$% 277 =G+&9%!+#-&$# 0.2 -77 -&%3# * + +' !'% "&7+&!- 9#'

3#3#-&%3' 8* #$-&%&3#$' !'% 0./? (>0)< 1./, (?0) = 2.4? (K0)

-77 $ ' !''#+ $ 63#!3' $ P"&#+& *%#. L# 7+&!- 8*

#+*#$# #+ -%&-' 0< >0< K0< 1,0< 210 = 20< $*: $ +# #$-&%&3#!&%

$ +# *3#%! &%$*!3'#. E3#$3&!#-%3 P"=#+& *%# %' %3'

#!!&% 8#-#!'+'7# &$%3 #+ *3&+&@# G+&9%!+#-&$# ,-7 !'-' 8;-#!'

8%!+. A$-; + 3*$&' 3'6&!'+7&!' $-'3 %&+ $ 3'6&!&$#$

#7*$# #*%3. E% !'%!+*&% +# $'& #$-&%&3#$# %' -'3#'% %&%7%

8!3' "&'7+&!-&!' #+ #$-&%&3#$' % #3'+'7# +#!&'%#$#< '

!'% '3' -'$+' 6&-%3#+ =# * !+-%3 +# 8#-&+

'+#%#!# #!3&&$#$ 8#-#!'+7&!#.

TITULO C1>Q YACON UN POTENCIAL PRODUCTO NATURAL

PARA EL TRATAMIENTO DE LA DIABETESRESUMEN

E% %*3' +#9'#3'&' "-' #%#+&@#$' +' 8!3' $+ !'%*-' '#+ *9Q

!%&!' $ "#&%# $ #! $ =#!%< !'-' *+-%3' $&3#&' % #3#

%'-#+. (G%3# 3 #+.< 200,). E *% '$*!3' 9&% 3'+#$' = %' ''!#

*3# %7#3&#< 3'6&!&$#$ ' 8!3' %*3&!&'%#+ #$'< % +# $'&

%#=#$#. N' %3# #!!&% "&'7+*!-%3< ' & '$*! *%#



7/18

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&7%&8&!#3&# $*!!&% % +' %&+ '3Q#%$+ $ 3&7+&!:&$'

:&!'. E3 8!3' #!'-#J# $ *%# $ *%# %'3#9+ #!3&&$#$ $ +#

-*!'# $+ !'+'% % 3:-&%' $ *%# "&3'8 $+ !&7'. R!&%3-%3

%!'%3 * + !'%*-' $ "#&%# $ =#!% 3&-*+# +# #9'!&% &%33&%#+

$ !#+!&' = '-* *% 9#+#%! #$!*#$' $ !#+!&'Q-#7%&'< '$*!&%$'

"*' -; #%' = &3%3< '9#9+-%3 $9&$' # *% &%!-%3' $ +#

*8&!& $ #9'!&% # %&+ $+ !&7' $ +' #%&-#+ 3#3#$'.(L'9' 3

#+.< 200) L# #$-&%&3#!&% *9Q!%&!# $ #! $ =#!% # #3# !'%

$3 6&-%3#+ '$*' *%# &7%&8&!#3&# $*!!&% $ +' %&+ $

3&7+&!:&$'< !'+3'+ 3'3#+< LDL = VLDL !'+3'+ % !'%$&!&'% $

#=*%'. (C#9# 3 #+ 200?). E3' $#3' 9&'*-&!'< #!'-#J#$' $

"&3'8 !!#+ %' -&3% *7& * + +#$' !'%3%&$' $ FOS $

+# #! $ =#!% '$# &%$*!& +# '+&8#!&% $+ !&7' '9#$#<

++#%$' # *% #*-%3' +'!#+ % +' %&+ $ :3&$' 1 -#%3 #

7+*!#7% (GLPQ1)< :3&$' &%33&%#+ * ' #!!&'% $&!3# '9 +

-3#9'+&-' $ +' +&$'.

TITULO C1>Q YACON UN POTENCIAL PRODUCTO NATURAL

PARA EL TRATAMIENTO DE LA DIABETES

RESUMEN:

E+ S-#++#%3* '%!"&8'+&* (=#!%) *%# +#%3# %#3*#+ $ +# 7&% #%$&%#<*3&+&@#$# '*+#-%3 ## + !'%3'+ $ +# $3 -++&3*. L' '93&'

$+ %3 3*$&' 8*'% $-'3# * + 63#!3' "&$'#+!'"+&!' #+

10 $ +# "'# $+ S-#++#%3* '%!"&8'+&* (=#!%) %3# #!3&&$#$

"&'7+*!-%3 % #3# !'% $3 -++&3* 3&' 2< $3-&%# *

'9#9+ -!#%&-' $ #!!&% = $'& 8!3&# $-'3# * %' %3#

#!3&&$#$ "&'7+*!-%3 % #3# !'% $3 -++&3* 3&' 1 $3-&%#



8/18

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* '&9+ 8!3' #$' '9 #+ 9&'*-&!# = "-#3'+7&!#

% #3# !'% $3 -++&3* 3&' 2. P## + 3*$&' $ $3 -++&3*

3&' 1 *#'% 4, #3# -#!"'< $3-&%#'% +' %&+ $ 7+*!'#

&%*+&%# % #%7. P## + 3*$&' $ $3 -++&3* 3&' 2 *#'% 24

#3# -#!"'< $3-&%#'% +' %&+ $ 7+*!'# % #%7< +' 8!3'

#$' # %&+ 9&'*-&!' = "-#3'+7&!'. L' *+3#$'< % +# $3

-++&3* 3&' 1 %' "*9' #!&% &7%&8&!#3&# $ +' %&+ $ 7+*!'# %

#%7 ( -#= # 0.0,)< +' %&+ $ &%*+&%# #*-%3#'%

&7%&8&!#3&#-%3 ( -%' # 0.0,). E% +# $3 -++&3* 3&' 2 +'

7*' $ =#!% = 7+&9%!+#-&$# $&-&%*='% &7%&8&!#3&#-%3 +'

%&+ $ 7+*!'# ( -%' # 0.001) +' 3*$&' $ 8!3' #$' #

%&+ 9&'*-&!' %' 6&3 $&8%! &7%&8&!#3&# ( -#=' # 0.0,) =< #

%&+ "-#3'+7&!' +' %&+ $ "-#3< "-'7+'9&%# = "-#3'!&3'

$&-&%*='% &7%&8&!#3&#-%3 % + 7*' $+ =#!% ( -%' # 0.0,).

C'%!+*&'% + 63#!3' "&$'#+!'"+&!' #+ 10 $ +# "'# $+

S-#++#%3* '%!"&8'+&* (=#!%) %3# #!3&&$#$ "&'7+*!-%3 % #3#

!'% $3 -++&3* 3&' 2< * '9#9+ -!#%&-' $ #!!&% ' *

-'#% +# !'%!%3#!&'% $ &%*+&%# % #%7< +# $'& 8!3&# %3

,00 = 1000 -77 $ ' !''#+< %' %3# #!3&&$#$ "&'7+*!-%3

% #3# !'% $3 -++&3* 3&' 1< %' %3# 8!3' #$'

&7%&8&!#3&'.

=.2 PROBLEMA

FORMULACION DEL PROBLEMA



9/18


T%$; -' 8!3' H&'7+*!-%3 + S&%7&-' $ A7*#=-#%3' =

G*#%;9#%# ' A7*#=-#%3' #'!$' #+ Y#!% % +#!&% # +#

G+&9%!+#-&$# % #3# H'+3@-#% &%$*!&$# # D3 -$%3

A+'6#%' % + 9&'3&' $ +# U%&&$#$ F$&!' V&++##+ % + 201,

HIPÓTESIS

H0te Ge'era!#

E+ S&%7&-' $ A7*#=-#%3' = G*#%;9#%# ' A7*#=-#%3' #'!$' #+

Y#!% 3%$; *% 8!3' &-&+# ' -' % +# "&'7+*!- * +#GLIBENCLAMIDA '9 +# $3 &%$*!&$# ' A+'6#%' % #3#

H'+3@-#%.

H0te E0e45a#

Ha E+ 8!3' H&'7+*!-%3 + S&%7&-' $ A7*#=-#%3' #'!$'

# +# G*#%;9#%# 3&% *% 8!3' &-&+# ' -' * + &%7&-' $+

A7*#=-#%3' #'!$' #+ Y#!%.

H0te N$!a#

Ho E+ 8!3' H&'7+*!-%3 + S&%7&-' $ A7*#=-#%3' #'!$'

# +# G*#%;9#%# %' 3&% *% 8!3' &-&+# ' -' * + &%7&-' $+

A7*#=-#%3' #'!$' #+ Y#!%.

• VARIABLESI%$%$&%3• E63#!3' $ ANNONA MURICATA L. (G*#%;9#%#)• E63#!3' $ PHYLASIS PERUVIA (A7*#=-#%3')• E63#!3' $ SMALLANTHUS SONCHIFOLIUS (Y#!%)• G+&9%!+#-&$#



10/18

1!


• S&%7&-' $ 63#!3' $ PHYLASIS PERUVIA = ANNONA

MURICATA L.• S&%7&-' $ 63#!3' $ PHYLASIS PERUVIA =

SMALLANTHUS SONCHIFOLIUS

D%$&%3• E8!3' "&'7+*!-%3 % +# $3 &%$*!&$# ' A+'6#%' % #3#

H'+3@-#%.

=., $ +#

'9+#!&% $:3&!# '!$ $ # &%$*3+&@#$' = *% ?, &% %

# % # $ $#'++'< +' % L#3&%'#-:&!# "#= #+$$' $ 1K

-&++'% $ '%# * #$!% +# %8-$#$. E% + P< !'%3&3*= *%#

%8-$#$ -*= #+%3 (.2)< &%$' P&*# + $#3#-%3' $ -;

#+3# &%!&$%! + 7*' 3;' -; #8!3#$' + $ ?, #J' # -;< !'% *%

&%!-%3' !'%&$#9+ % '%# %3 20 = 4K #J' $ $#$.



11/18

11


E% +# #!3*#+&$#$ 3;% %#=#%$' -*!"' 3#3#-&%3'< $$

!'-9&%#!&% $ $&3#< $*!#!&%< !&!&' 8&!'< &%-*%'3#< "#3#

3#%+#%3 #%!;3&!' ' +# &%3&7#!&% 3; &%!&#+-%3

'&%3#$# # '93% %*# $'7# "&'7+&!-%3 * *$#% !'%3'+#

3# %8-$#$. S&% -9#7'< % %*3' # = % '3' # !'% %&+

'!&'!'%-&!' -#%3< *% 7#% !3' $ +# '9+#!&% %' 3&% #!!' #

3' -'$%' *-# $ 3#3#-&%3'< ' +# +&-&3#!&'% !'%-&!# =

!*+3*#+< *7&%$' %3'%! +# 8&3'3# ' -$&!&%# %#3*#+ !'-' *%#

#+3%#3&# #!!&9+< !'% &7*#+ 8!3&&$#$ * +' '$*!3' &%3:3&!'< '

&% +' 3-&9+ 8!3' !*%$#&' 3&!' $ $&!"# $'7#< = !'% +#%3## $ -; !'%-&!#.

L# +#%3# -$&!&%#+ *$% '3&-&@# + -3#9'+&-' $ +# 7+*!'# = +#

!'%$&!&% &%37#+ $ +' $:3&!'< %' '+' ' * 8!3'

"&'7+&!-%3 &%' 3#-9&:% #+ -'# + 8&+ +&$&!'< + 3#$'

#%3&'6&$#%3 = +# 8*%!&% !#&+#. A %&+ -*%$+ "#% '3#$' -; $

400 '$*!3' 9'3;%&!' ## +# $3. U%' $ +' -; 3*$$' +

7&%%7< + !*#+ $-'3 *%# $*!!&% %3 1/ >1 $+ ;# 9#' +#

!*# $ 7+&!- !*#%$' #$-&%&3 40 -&%*3' #%3 $ *%# '9!#7#

$ 7+*!'#.

P"=#+& *%#< !'%'!&$# !'-%-%3 !'-' #7*#=-#%3'< *!"*#<

3'-#3 &+3< 3!. *%# +#%3# '&7&%# $ +' A%$ P*#%'

3%!&%3 # +# 8#-&+ $ +# S'+#%#!#< = !'% #+3' '3%!+ $

-*+3&+&!#!&% =# * !! % *+' '9. U%# +#%3# *$ '$*!&

!!# $ >00 8*3'< * '% 9#=# $ !'+' %##%#Q#-#&++'< $ 8'-#

7+'9'#< !'% *% ' %3 4Q, 7 = #9' #7&$*+!. E 63-#$#-%3 &!#

% V&3#-&%# A (?4/ UI 1007) = 3&% 9*%' !'%3%&$' $ V&3#-&%# C

(2?-7)< 8&9# (4./7)< '3%# (1.K7)< 88''< "&'< '3#&' = @&%!. S +

"#% #3&9*&$' -*!"# '&$#$ -$&!&%#+ 3#+ !'-' #%3-;3&!'<

$&*:3&!'< #%3&:3&!'< $#%3< #%#+7:&!'< 8'3&8&!# + %&' 3&!'< #+&



12/18

12


'9+-# $ 7#7#%3#< +&-&%# #;&3' &%33&%#+ = #-9# #$-;

'3#% * '&$#$ #%3&$:3&!#< !'-%$#%$' + !'%*-' $ ,

8*3' $&'. N' 6&3&%$' 3*$&' &' * &%$&*% * '&9+

8!3' #$'.

E+ S-#++#%3"* '!"&8'+&* (=#!%) *%# +#%3# %%< "9;!#<

3%! # +# 8#-&+ A3#!#< $&3&9*= #-+-%3 % +# 7&'%

#%$&%#< !'-%$&%$' # V%@*+#< C'+'-9< P< B'+& "#3#

A7%3&%# (S-&%#&' = !'+. 200>). S* !*+3&' 3; % #*-%3' $9&$' # *

"# '3*+#$' * * #! = * "'# 3&%% 8!3' '9 +# #+*$

"*-#%#< % !+ '9 +# $3 -++&3* (M#%&* = !'+. 200>)

'&$#$ * "#% !'%3&$' #+ =#!% % *% !*' &-'3#%3 ## +#

'%# * #$!% $3.

E3*$&' +&-&%# "#% $-'3#$' * +# "'# $ =#!% $*!% +'

%&+ $ 7+*!'# % #3# 6&-%3#+-%3 $:3&!#< -#%3&%% +#

!##!&$#$ 8&+3#%3 $ +' &J'% (A=9# 3 #+. 2001) = %' #+3#% +' %&+

%'-#+ $ 7+*!'# +#-;3&!# (V'+#3' 3 #+. 1KK) ' '3' +#$' % *%

3*$&' +&-&%# !+%&!' $-'3#'% * +# #! 3&%% #!3&&$#$

"&'7+*!-%3 (M#=3# = !'+. 2001).

L# "'# = 9'3 3&%' $ A%%'%# -*&!#3# (7*#%;9#%#) '% *#$' '

#+7*%# !'-*%&$#$ !'-' #%3&!#%!7%'< #%3&#-$&!'< $#3&'<

#%3&-#+;&!'< #'$&+#3#$' = #%3&$:3&!' = +' 3*$&'

6&-%3#+ "#% $-'3#$' +# 8&!#! "&'7+&!-%3 #+ *3&+&@# +

63#!3' 3#%+&!' $ +# "'# % #3# $:3&!# &%$*!&$# ' #+'6#%'< &%

#8!3# + "7#$' = &J%.

E #9&$' * +# 7+&9%!+#-&$# *%# $ +# &%!&#+ !#*# $

"&'7+&!- &%$*!&$# ' $'7#< ' +' * + *' $ 3 -$&!#-%3'

*$ !#*# 3-9+'< -#'< $++' $ !#9@#< %3 '3' %3'-#< '

* 8!3' "&'7+&!-%3 +' "#! % # ''3*%&$#$ + 3#3#-&%3'

&$#+ ## #!&%3 $%'3&!#$' !'% $3.



13/18

13


D9&$' # * +# $3 -7 !'-' *%# $ +# -#=' !#3;3'8 $

#+*$ * "# &3' + -*%$' 3#%3' ' * -'3#+&$#$< -'9&+&$#$ &-#!3'

% +# !#+&$#$ $ &$# !'-' ' +# $&!##!&$#$ * 7%# = + #+3&-'

!'3 '%#+< '!+ = !'%-&!' #$-; * '!%3# $ #+%!

3;% #*-%3#%$' ;&$#-%3 % *%# '9+#!&% +#3&#-%3 '% =

'$*!3&# # * *7 +# %!&$#$ $ 3*$ + 8!3' $ 3' 8*3'

## #+&$# !&%38&!#-%3 * 8!3&&$#$< !'-%$# * *' = $*!& +

!'3' $+ !*&$#$' $ +# #+*$ $ 3' #!&%3.

D &7*#+ -#%# $9&$' # 3' #%3!$%3 = '3' 3*$&' +#!&'%#$' #

+# !'-'&!&% $ +' 8*3' $3 + &%3: $+ %3 3*$&'< + !*#+

3; '&%3#$' # %!'%3# &$%! '9 + *' 7*' $ 63#!3' $ +'

8*3'< #+ #+*# * #!3&&$#$ "&'7+*!-%3 % -'$+' $ $3

-++&3* 3&' 1 = 2 % #3# = * '&9+ 8!3' #$' * *$&#%

%3#.

=.= OB6ETIVOS

OBETIVO GENERAL

D3-&%# & 3&% -' 8!3' "&'7+*!-%3 + &%7&-' $

63#!3' $ physalis peruviana (#7*#=-#%3') = annona muricata

(7*#%;9#%#) ' $ physalis peruviana = smallanthus sonchifolius (=#!%)

% +#!&% # +# 7+&9%!+#-&$# % #3# H'+3@#-% &%$*!&$# # $3

-$%3 #+'6#%' % + 9&'3&' $ +# U%&&$#$ F$&!' V&++##+ %

+ 201,.

OBETIVOS ESPECIFICOS



14/18

14


1. D3-&%# & + 8!3' "&'7+*!-%3 $+ 63#!3' $ physalis

peruviana (#7*#=-#%3') ; -#=' * + $ +# 7+&9%!+#-&$# % +#

$3 &%$*!&$# ' #+'6#%' % #3#.2. D3-&%# & + 8!3' "&'7+*!-%3 $+ 63#!3' $ annona

muricata (7*#%;9#%#) ; -#=' * + $ +# 7+&9%!+#-&$# % +#

$3 &%$*!&$# ' #+'6#%' % #3#.>. D3-&%# & + 8!3' "&'7+*!-%3 $+ 63#!3' $ smallanthus

sonchifolius (=#!%) ; -#=' * + $ +# 7+&9%!+#-&$# % +#

$3 &%$*!&$# ' #+'6#%' % #3#.

4. D3-&%# + 8!3' "&'7+*!-%3 $ +# &%7 $+ physalis

peruviana #'!$' !'% annona muricata 8%3 # +# 7+&9%!+#-&$#.,. D3-&%# + 8!3' "&'7+*!-%3 $ +# &%7 $+ physalis

peruviana #'!$' !'% smallanthus sonchifolius 8%3 # +#

7+&9%!+#-&$#.

=.5 MTODO

S *3&+&@#; 0 #3# #@# H'+3@-#% '!$%3 $+ INS (I%3&3*3' N#!&'%#+ $

S#+*$)< -#!"' $ 200 Q ,0. L' #%&-#+ ;% #+&-3#%$' !'% $&3# =

#7*# #$ +&&3*-. 24 "'# #%3 $+ %#=' + '-3; # #=*%' !&9&%$'

#7*# # +&93#$. M$%3 !;%*+# -3;+&!# #$-&%&3#; ' # '#+ #+'6#%'

-'%'"&$#3#$' # *%# $'& $ 1>0-77 $&*+3' % #7*# $3&+#$# '

&%3#&3'%#+<

S $&&$&' # +' #%&-#+ % 7*' $ 10 !#$# *%'< 7% + &7*&%3

$&J'

1 G*' !'%3'+ RATAS DIABETICA (RD) !'% '+*!&% #+&%# 8&&'+'7&!#



15/18

15


2 P#3% #%&-#+ 3#3#$' %&!#-%3 !'% + -$&!#-%3'

GLIBENCLAMIDA (,-77)> G*'1 A%&-#+ 3#3#$' !'% + 63#!3' ETANOLICO DE ANNONA

MURICATA L. # *%# !'%!%3#!&% $4 G*' 2 A%&-#+ 3#3#$' !'% + 63#!3' PHYLASIS PERUVIA

(A7*#=-#%3') # *%# !'%!%3#!&% $, G*' > A%&-#+ 3#3#$' !'% + 63#!3' SMALLANTHUS

SONCHIFOLIUS (Y#!%)? G*' 4 A%&-#+ 3#3#$' !'% +# &%7 $ AGUAYMANTO Y

GUANABANA # *%# !'%!%3#!&% $ ..

G*', A%&-#+ 3#3#$' !'% +# &%7 $ AGUAYMANTO Y

YACONA+ 7*' !'%3'+ +' + #$-&%&3#; *' 8&&'+7&!' !#$# ? "'# 2 !

#+ $# +# $'& $ 10-+ 7 % '. A %*3' 7*' #3% + #$-&%&3#;

'-#@'+ $ 20 -7 7 $&+*&$' % #7*# $3&+#$#< *%# @ #+ $#.

L*7' $ , $ 8*'% #%3$' !'% 1-+ $ %3'9#9&3#+ $&!' (H#+#3#+)<

+*7' '!$& # !&*7# #9$'-&%#+ ## +# +'!#+&@#!&% = 63#!!&% $+

"7#$' &J'< = ;%!# '3&'-%3 8*'% +##$' !'% *' 8&&'+7&!' =

63%$&$' ## +'7# *%# !'-+3# 6'&!&% ## + #%;+&& -#!'!&!'.

P## +#. 1KK/< +' "&7#$' &J'% = ;%!# 8*'% 63&#$' = +##$'

*#-%3 !'% '+*!&% #+&%#< ## -' +# #%7 = +' $3&3' #$"&$'

#+ 3&$'. S +' 8& % '+*!&% $ 8'-'+ #+ 10 3#-'%#$< ' 12 "'# =

+*7' +!!&'%' +# @'%# $'%$ $ #!*$' #+ 6#-% -#!'!&!'

&$%! +&'% $ ##%3. T#%!*&$' 3 3&-'< + '!' % *%

#*3'3!%&!% ' 1/ "'#< % '+*!&'% $ #+!'"'+ ## $"&$#3#!&%<

"&$#3#!&% !'% 6&+'+< = -99&$' % ##8&%#. E+ '!#-&%3' $ +' 3#!' $ ##8&%# #+&@ -+#%$' *% $&%#$' $ ##8&%#. L' 3#!' '93%&$'



16/18

16


8*'% ++#$' # !'%7+#-&%3' W 0C< ' *% +#' $ > "'#< ## +*7'

'!$ #+ !'3 $ > um $ ' !'% #=*$# $ -&!3'-. L*7'< 3&

+# ##8&%# % *%# 3*8# ## '!$ # +# !'+'#!&% !'% "-#3'6&+&%# =

'&%#. D*: $ +# $"&$#3#!&% = +&-&@# $ +;-&%#< 8*'% ++#$'

## '9#!&% -&!'!&!#.

4.? METODOLOGA

A) TIPO Y DISEÑO METODOLÓGICO

S7% + 3&-' $ '!*%! $ +' "!"' = 7&3' $ +#

&%8'-#!&% PROSPECTIVO.

S7% + &'$' = !*%! $+ 3*$&' TRANSVERSAL.

S7% + #%;+&& = #+!#%! $ +' *+3#$' EXPERIMENTAL.

B) ÁREA DE ESTUDIOE+ 3*$&' $ &%3&7#!&% #+&@#; % + B&'3&' $ +# F#!*+3#$ $

M$!&%# $ +# U%&&$#$ N#!&'%#+ F$&!' V&++##+.

C) POBLACIÓN Y MUESTRAU,?&%'$ 4& &'#"4,$

R#3# -#!"' H'+3@-#% $ 200 5 2,0 7. A!3*#+-%3 +# #3# *%'

$ +' #%&-#+ $ -#=' 8%! ## +# &%3%!&'%

6&-%3#+< = *3&+&@# '* $ 8;!&+ -#%&*+#!&% ' *

#+3# !##!&$#$ $ '$*!!&%< #$-; $&!"' -#-8' !'-#3

!##!33&!# -3#9+&!# &-&+# #+ "*-#%'.

(S"#8& 3. A+ 1KKK)



17/18

17


T**$ 4& * "&'#%*

E% +' 3*$&' 6&-%3#+ + %-' #$!*#$' $ *3' '

*%&$#$ 6&-%3#+ $%$ $ +# 3:!%&!# 3#$3&!# !'% *

#%#+&!% +' $#3' % 7%#+ ## +# -#='# $ +'

6&-%3#$' + -%&-' $+ 3#-#J' -*3#+ !'-%-%3

#!3#$' $ 10.

M*3# #*3#$# # +# $&$#

n( 1

1− R )

D#'% S#*%$ = T#Q200,

% N*-' $ *3' &% $&$#.

R P''!&% #$# $ :$&$#.

P## 8!3' $+ 3*$&'

10(11Q0.10) 11 ' !#$# 7*'.

D) CRITERIOS DE INCLUSIÓN Y ECLUSIÓNCRITERIOS DE INCLUSIÓN• L' #%&-#+ *3&+&@#$' ## + $#'++' $+ %3 3#9#' '% #3#

-#!"' $ +# #&$#$ H'+3@-#%< ##%3-%3 #%# = !'% *% '

'-$&' $ 200 5 2,0 7. -#%3%&$' % *% :7&-% $ +&9 #!!' #+

#+&-%3' = + #7*# !'% *% !&!+' $ +*@ = '!*&$#$ $ 12 "'#

!3&#-%3.• S $!&$&' 3#9## !'% #3# H'+3@-#%< $9&$' # * %3#% *%# 7#%

&-&+&3*$ 7%3&!# !'% +# !& "*-#%#.

CRITERIOS DE ECLUSIÓN



18/18


• P' &%8&' $ 200 7.• R#3# 3%!&%3 # '3# #&$#$.• R#3# "-9#.• P%! $ %8-$#$ !'%!'-&3#%3 * #8!3 ' &%8+*=# % +#

$&#93.

E) PLAN DE RECOLECCION PROCESAMIENTO Y

PRESENTACIÓN DE DATOS

S !'%3#; !'% + #'=' $+ +#9'#3'&' $ 8#-#!'7%' = -$&!&%#

3#$&!&'%#+ $ +# UNMSM ## +# ##!&% $+ 63#!3' $ #%%'%#

-*&!#3# # !&3# = !''$&%#!&% !'% '%#9+ $+ 3#9+!&-&%3'

% -%!&%.

L# !'+!!&% $ $#3' 3'-## 3#%!*&$' +' $ $ &%&!$' +

6&-%3'.

S 3&-# *% 3&-' '-$&' ## +# !'+!!&% $ $#3' % $ $*#%3

+ - $ *+&' $+ %3 #J'. L' $# = "'#&' %!#&' ##

$#'++# 3# #!3&&$#$ $%$;% $ +# 6&7%! $+ 3*$&' #& !'-'

$ +# '%# '%#9+ $+ +#9'#3'&'.

E &-'3#%3 -%!&'%# * !'%3## !'% + #'=' $ '8&'%#+

63' % +# -3'$'+'7# $ &%3&7#!&% &% &' %3 ++' 9&+'7#<

*&-&!' 8#-#!:*3&!'< +#9'#3'&3#< #3+'7' %3 '3'.


proyecto de investigación del año academico 2015

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