Ómica y Parásitos en ICOPA XIIIcon referencia a Taenia solium

Dr. Juan Pedro LacletteInstituto de Investigaciones Biomédicas

Universidad Nacional Autónoma de México

15 de Abril 2015

Genoma (constituido por DNA), es el conjunto de genes.

Transcriptoma, es el conjunto de RNA mensajeros de esos genes.

Proteoma, es el conjunto de todas las proteínas que se expresan en un momento en un tipo celular o en un organismo.

Metaboloma, es el conjunto de los metabolitos en un tipo celular o en un organismo; estos metabolitos pueden tener una diversa naturaleza química.

¿Cómo se definen? (ejemplos)

Algunas fechas:• 1985 Primera iniciativa para secuenciar los 3,200 millones de nucleótidos del genoma humano. • 1990 se inició el Proyecto del Genoma Humano, Este Proyecto contó con la participación de universidades de los EUA, Reino Unido, Francia, Alemania, China y Japón, etc.• 2003 se reporta el primer borrador del Genoma Humano• 2014 se han reportado más de 240 genomas de eucariotes y más de mil genomas bacterianos.• 2015 el costo actual para secuenciar el genoma propio, para cualquiera de nosotros, es de alrededor de 11,000 USD; se estima que pronto el costo se vuelva más accesible.

El PGH desató una revolución Científico-Tecnológica

Una revolución Científico-Tecnológica en curso

Palabra clave Número de artículos en PubMed

Genomic 1 097 374

Genomic y Medicine 370 456

Genomic y Analysis 520 097

Genomic y Parasite 12 543

Esta revolución científica se refleja en las publicaciones

Acumulado de los proyectos de genomas en eucariotes

Pareek et al., J Appl Genet. 2011

Revolución tecnológica en la secuenciación del ADN

Plataforma Producción Longitud Calidad Costos Aplicaciones Posibles Fuentes de error

Comparación costo/eficiencia entre diferentes plataformas de secuenciación

Organism/Name Kingdom Group SubGroup Size (Mb) Chr Organelles Plasmids Assemblies Phytomonas serpens Eukaryota Protists Kinetoplasts 25.6932 - - - 1Phytophthora alni Eukaryota Protists Other Protists 236 - - - 1Phytophthora cambivora Eukaryota Protists Other Protists 230.616 - - - 1Phytophthora capsici Eukaryota Protists Other Protists 56.0343 - - - 1Phytophthora cinnamomi Eukaryota Protists Other Protists 16.7332 - - - 1Phytophthora cryptogea Eukaryota Protists Other Protists 103.037 - - - 1Phytophthora fragariae Eukaryota Protists Other Protists 73.6803 - - - 1Phytophthora infestans Eukaryota Protists Other Protists 228.544 - 1 - 1Phytophthora kernoviae Eukaryota Protists Other Protists 43.2952 - - - 6Phytophthora lateralis Eukaryota Protists Other Protists 56.3096 - - - 5Phytophthora parasitica Eukaryota Protists Other Protists 82.3892 - - - 9Phytophthora pinifolia Eukaryota Protists Other Protists 131.905 - - - 1Phytophthora pisi Eukaryota Protists Other Protists 58.8567 - - - 1Phytophthora ramorum Eukaryota Protists Other Protists 66.6524 - 1 - 3Phytophthora rubi Eukaryota Protists Other Protists 47.6718 - - - 1Phytophthora sojae Eukaryota Protists Other Protists 82.5976 - 1 - 1Phytopythium vexans Eukaryota Protists Other Protists 33.8449 - - - 1Plasmodium berghei Eukaryota Protists Apicomplexans 17.9546 - 1 - 1Plasmodium chabaudi Eukaryota Protists Apicomplexans 16.8934 - 2 - 1Plasmodium coatneyi Eukaryota Protists Apicomplexans 27.8175 14 - - 1Plasmodium cynomolgi Eukaryota Protists Apicomplexans 26.1813 14 - - 1Plasmodium falciparum Eukaryota Protists Apicomplexans 27.0235 14 1 - 23Plasmodium fragile Eukaryota Protists Apicomplexans 25.9145 - 1 - 1Plasmodium gaboni Eukaryota Protists Apicomplexans 16.3031 - - - 1Plasmodium inui Eukaryota Protists Apicomplexans 27.405 - - - 1Plasmodium knowlesi Eukaryota Protists Apicomplexans 23.4622 14 1 - 1Plasmodium reichenowi Eukaryota Protists Apicomplexans 24.0555 - 1 - 1Plasmodium vinckei Eukaryota Protists Apicomplexans 18.9315 - - - 2Plasmodium vivax Eukaryota Protists Apicomplexans 28.806 14 1 - 5Plasmodium yoelii Eukaryota Protists Apicomplexans 23.1254 - - - 2Polysphondylium pallidum Eukaryota Protists Other Protists 32.9702 - 1 1 1Polysphondylium violaceumEukaryota Protists Other Protists 25.7234 - - - 1Pseudoperonospora cubensisEukaryota Protists Other Protists 64.3328 - - - 1Pythium aphanidermatum Eukaryota Protists Other Protists 35.8768 - - - 1Pythium arrhenomanes Eukaryota Protists Other Protists 44.6726 - - - 1Pythium insidiosum Eukaryota Protists Other Protists 45.6097 - - - 1Pythium irregulare Eukaryota Protists Other Protists 42.9681 - - - 1Pythium iwayamai Eukaryota Protists Other Protists 43.1992 - - - 1Pythium ultimum Eukaryota Protists Other Protists 44.9135 - 1 - 2Reticulomyxa filosa Eukaryota Protists Other Protists 101.867 - - - 1Salpingoeca rosetta Eukaryota Protists Other Protists 55.4403 - - - 1Saprolegnia diclina Eukaryota Protists Other Protists 62.8858 - - - 1Saprolegnia parasitica Eukaryota Protists Other Protists 53.1316 - - - 1Sarcocystis neurona Eukaryota Protists Apicomplexans 130.023 - - - 2Schizochytrium Eukaryota Protists Other Protists 39.0897 - - - 1Spironucleus salmonicida Eukaryota Protists Other Protists 12.9546 - - - 1Strigomonas culicis Eukaryota Protists Kinetoplasts 27.2838 - - - 2Strigomonas galati Eukaryota Protists Kinetoplasts 27.235 - - - 1Strigomonas oncopelti Eukaryota Protists Kinetoplasts 24.9629 - - - 1Stylonychia lemnae Eukaryota Protists Other Protists 50.1645 - - - 2Symbiodinium minutum Eukaryota Protists Other Protists 609.476 - - - 1Tetrahymena borealis Eukaryota Protists Other Protists 93.5061 - - - 1Tetrahymena elliotti Eukaryota Protists Other Protists 90.8376 - - - 1Tetrahymena malaccensis Eukaryota Protists Other Protists 106.672 - 1 - 1Tetrahymena thermophila Eukaryota Protists Other Protists 157.693 - - - 2Thecamonas trahens Eukaryota Protists Other Protists 28.6806 - 1 - 1Theileria annulata Eukaryota Protists Apicomplexans 8.35843 5 - - 1Theileria orientalis Eukaryota Protists Apicomplexans 9.01036 4 - - 1Theileria parva Eukaryota Protists Apicomplexans 8.34761 2 1 - 1Toxoplasma gondii Eukaryota Protists Apicomplexans 65.0622 - 1 - 16Trichomonas vaginalis Eukaryota Protists Other Protists 176.42 - - - 1Trypanosoma brucei Eukaryota Protists Kinetoplasts 26.0755 11 - - 2Trypanosoma congolense Eukaryota Protists Kinetoplasts 18.8055 - - - 1Trypanosoma cruzi Eukaryota Protists Kinetoplasts 89.9375 - - - 7Trypanosoma grayi Eukaryota Protists Kinetoplasts 20.9341 - - - 1Trypanosoma rangeli Eukaryota Protists Kinetoplasts 15.1143 - - - 1Trypanosoma vivax Eukaryota Protists Kinetoplasts 24.7793

Esta revolución ciientífico-tecnológica también llegó a la Parasitología:Genomas de protistas en la base de datos del NCBI (167)

Organism/Name Kingdom Group SubGroup Size (Mb) Chr Organelles Plasmids Assemblies Acanthamoeba astronyxis Eukaryota Protists Other Protists 83.4325 - - - 1Acanthamoeba castellanii Eukaryota Protists Other Protists 115.05 - 1 - 3Acanthamoeba culbertsoni Eukaryota Protists Other Protists 55.5438 - - - 1Acanthamoeba divionensis Eukaryota Protists Other Protists 84.7694 - - - 1Acanthamoeba healyi Eukaryota Protists Other Protists 75.3182 - - - 1Acanthamoeba lenticulata Eukaryota Protists Other Protists 66.0257 - - - 1Acanthamoeba lugdunensis Eukaryota Protists Other Protists 99.4171 - - - 1Acanthamoeba mauritaniensis Eukaryota Protists Other Protists 106.836 - - - 1Acanthamoeba palestinensis Eukaryota Protists Other Protists 103.483 - - - 1Acanthamoeba pearcei Eukaryota Protists Other Protists 115.614 - - - 1Acanthamoeba polyphaga Eukaryota Protists Other Protists 120.415 - - - 1Acanthamoeba quina Eukaryota Protists Other Protists 83.589 - - - 1Acanthamoeba rhysodes Eukaryota Protists Other Protists 75.8213 - - - 1Acanthamoeba royreba Eukaryota Protists Other Protists 79.5441 - - - 1Acytostelium subglobosum Eukaryota Protists Other Protists 31.003 - - - 1Albugo candida Eukaryota Protists Other Protists 33.8236 - - - 4Angomonas deanei Eukaryota Protists Kinetoplasts 34.1038 - - - 2Angomonas desouzai Eukaryota Protists Kinetoplasts 24.2509 - - - 1Aphanomyces astaci Eukaryota Protists Other Protists 75.8444 - - - 1Aphanomyces invadans Eukaryota Protists Other Protists 71.4025 - - - 1Ascogregarina taiwanensis Eukaryota Protists Apicomplexans 6.14941 - - - 1Astrammina rara Eukaryota Protists Other Protists 1.45009 - - - 1Aureococcus anophagefferens Eukaryota Protists Other Protists 56.6606 - 1 - 1Babesia bigemina Eukaryota Protists Apicomplexans 13.8409 - - - 1Babesia bovis Eukaryota Protists Apicomplexans 8.17971 2 2 - 1Babesia divergens Eukaryota Protists Apicomplexans 11.3828 - - - 1Babesia equi Eukaryota Protists Apicomplexans 11.6745 2 - - 1Babesia microti Eukaryota Protists Apicomplexans 6.39244 3 1 - 1Blastocystis Eukaryota Protists Other Protists 15.4178 - 1 - 7Blastocystis hominis Eukaryota Protists Other Protists 18.8172 - - - 1Capsaspora owczarzaki Eukaryota Protists Other Protists 27.9678 - - - 1Chromera velia Eukaryota Protists Other Protists 187.455 - 1 - 1Crithidia acanthocephali Eukaryota Protists Kinetoplasts 33.7832 - - - 1Crithidia fasciculata Eukaryota Protists Kinetoplasts 40.2474 - - - 1Cryptomonas paramecium Eukaryota Protists Other Protists 0.487066 3 1 - 1Cryptosporidium Eukaryota Protists Apicomplexans 9.50978 - - - 1Cryptosporidium hominis Eukaryota Protists Apicomplexans 9.05401 - - - 2Cryptosporidium muris Eukaryota Protists Apicomplexans 9.24525 - - - 1Cryptosporidium parvum Eukaryota Protists Apicomplexans 9.10232 8 - - 2Cyclospora cayetanensis Eukaryota Protists Apicomplexans 46.155 - - - 1Dictyostelium citrinum Eukaryota Protists Other Protists 26.2518 - 1 - 1Dictyostelium discoideum Eukaryota Protists Other Protists 34.205 6 1 1 1Dictyostelium fasciculatum Eukaryota Protists Other Protists 31.0192 - 1 - 1Dictyostelium firmibasis Eukaryota Protists Other Protists 30.5597 - - 1 1Dictyostelium intermedium Eukaryota Protists Other Protists 30.5295 - - - 1Dictyostelium purpureum Eukaryota Protists Other Protists 32.9672 - - - 1Eimeria acervulina Eukaryota Protists Apicomplexans 45.8306 - - - 1Eimeria brunetti Eukaryota Protists Apicomplexans 66.8902 - - - 1Eimeria maxima Eukaryota Protists Apicomplexans 45.9751 - - - 1Eimeria mitis Eukaryota Protists Apicomplexans 60.4151 - - - 1Eimeria necatrix Eukaryota Protists Apicomplexans 55.0079 - - - 1Eimeria nieschulzi Eukaryota Protists Apicomplexans 62.8325 - - - 1Eimeria praecox Eukaryota Protists Apicomplexans 60.0833 - - - 1Eimeria tenella Eukaryota Protists Apicomplexans 51.8596 1 1 - 2Emiliania huxleyi Eukaryota Protists Other Protists 167.676 - 1 - 1Endotrypanum monterogeii Eukaryota Protists Kinetoplasts 32.5193 - - - 1Entamoeba dispar Eukaryota Protists Other Protists 30.6332 - - - 1Entamoeba histolytica Eukaryota Protists Other Protists 20.8354 - - - 5Entamoeba invadens Eukaryota Protists Other Protists 40.8783 - - - 1Entamoeba nuttalli Eukaryota Protists Other Protists 14.4 - - - 1Fonticula alba Eukaryota Protists Other Protists 31.2965 - - - 1Giardia intestinalis Eukaryota Protists Other Protists 12.0096 - - - 5Gregarina niphandrodes Eukaryota Protists Apicomplexans 14.0091 - - - 1Hammondia hammondi Eukaryota Protists Apicomplexans 67.7014 - - - 2Hemiselmis andersenii Eukaryota Protists Other Protists 0.632425 3 1 - 1Herpetomonas muscarum Eukaryota Protists Kinetoplasts 30.8448 - - - 1Heterococcus Eukaryota Protists Other Protists 60.7427 - - - 1Hyaloperonospora arabidopsidis Eukaryota Protists Other Protists 78.3805 - - - 1Hyphochytrium catenoides Eukaryota Protists Other Protists 85.1426 - - - 1Ichthyophthirius multifiliis Eukaryota Protists Other Protists 48.8 1 1 - 1Leishmania Eukaryota Protists Kinetoplasts 30.873 - - - 1Leishmania aethiopica Eukaryota Protists Kinetoplasts 31.9862 - - - 1Leishmania amazonensis Eukaryota Protists Kinetoplasts 29.0293 - - - 1Leishmania arabica Eukaryota Protists Kinetoplasts 31.4384 - - - 1Leishmania braziliensis Eukaryota Protists Kinetoplasts 35.2112 34 - - 2Leishmania donovani Eukaryota Protists Kinetoplasts 32.445 36 - - 5Leishmania enriettii Eukaryota Protists Kinetoplasts 30.7805 - - - 1Leishmania gerbilli Eukaryota Protists Kinetoplasts 31.3986 - - - 1Leishmania infantum Eukaryota Protists Kinetoplasts 32.1221 36 - - 1Leishmania major Eukaryota Protists Kinetoplasts 32.8551 36 - - 3Leishmania mexicana Eukaryota Protists Kinetoplasts 32.1087 34 - - 1Leishmania panamensis Eukaryota Protists Kinetoplasts 31.2639 35 - - 2Leishmania tropica Eukaryota Protists Kinetoplasts 32.989 - - - 1Leishmania turanica Eukaryota Protists Kinetoplasts 32.32 - - - 1Lotmaria passim Eukaryota Protists Kinetoplasts 32.813 - - - 1Mastigamoeba balamuthi Eukaryota Protists Other Protists 48.5931 - - - 1Monosiga brevicollis Eukaryota Protists Other Protists 41.7099 - 1 - 1Naegleria fowleri Eukaryota Protists Other Protists 27.7913 - 1 - 1Naegleria gruberi Eukaryota Protists Other Protists 40.9641 - 1 1 1Nannochloropsis gaditana Eukaryota Protists Other Protists 33.987 21 2 - 2Nannochloropsis oceanica Eukaryota Protists Other Protists 27.6399 - 2 - 1Neospora caninum Eukaryota Protists Apicomplexans 57.5474 14 - - 1Oxytricha trifallax Eukaryota Protists Other Protists 496.291 1 - - 2Paramecium biaurelia Eukaryota Protists Other Protists 76.9631 - - - 1Paramecium caudatum Eukaryota Protists Other Protists 30.4809 - 1 - 1Paramecium sexaurelia Eukaryota Protists Other Protists 68.0166 - - - 1Paramecium tetraurelia Eukaryota Protists Other Protists 72.0945 - - - 2Perkinsus marinus Eukaryota Protists Other Protists 86.6051 - - - 1Physarum polycephalum Eukaryota Protists Other Protists 205.176 - 1 1 1Phytomonas Eukaryota Protists Kinetoplasts 17.7809 - - - 1

Organism/Name Kingdom Group SubGroup Size (Mb) Chr Organelles Plasmids Assemblies Ancylostoma ceylanicum Eukaryota Animals Roundworms 348.995 - - - 2Ancylostoma duodenale Eukaryota Animals Roundworms 332.876 - 1 - 1Angiostrongylus cantonensis Eukaryota Animals Roundworms 253.243 - 1 - 2Angiostrongylus costaricensis Eukaryota Animals Roundworms 262.783 - 1 - 1Anisakis simplex Eukaryota Animals Roundworms 126.87 - 1 - 1Ascaris lumbricoides Eukaryota Animals Roundworms 316.975 - 1 - 1Ascaris suum Eukaryota Animals Roundworms 269.56 - 1 - 3Brugia malayi Eukaryota Animals Roundworms 93.6591 - 1 - 1Brugia pahangi Eukaryota Animals Roundworms 90.5451 - - - 1Brugia timori Eukaryota Animals Roundworms 64.9307 - - - 1Bursaphelenchus xylophilus Eukaryota Animals Roundworms 73.0857 - 1 - 1Caenorhabditis angaria Eukaryota Animals Roundworms 79.7615 - - - 1Caenorhabditis brenneri Eukaryota Animals Roundworms 190.37 - - - 1Caenorhabditis elegans Eukaryota Animals Roundworms 101.169 6 1 - 2Caenorhabditis japonica Eukaryota Animals Roundworms 166.256 - - - 1Caenorhabditis remanei Eukaryota Animals Roundworms 145.443 - - - 1Caenorhabditis tropicalis Eukaryota Animals Roundworms 79.3214 - 1 - 1Cylicostephanus goldi Eukaryota Animals Roundworms 173.362 - - - 1Dictyocaulus viviparus Eukaryota Animals Roundworms 160.964 - 1 - 1Dracunculus medinensis Eukaryota Animals Roundworms 103.751 - 1 - 1Elaeophora elaphi Eukaryota Animals Roundworms 82.5683 - - - 2Enterobius vermicularis Eukaryota Animals Roundworms 150.051 - 1 - 1Globodera pallida Eukaryota Animals Roundworms 123.625 - - - 1Gongylonema pulchrum Eukaryota Animals Roundworms 322.334 - 1 - 1Haemonchus contortus Eukaryota Animals Roundworms 319.758 - 1 - 2Haemonchus placei Eukaryota Animals Roundworms 259.126 - - - 1Heligmosomoides polygyrus Eukaryota Animals Roundworms 560.662 - - - 1Heterodera glycines Eukaryota Animals Roundworms 81.908 - - - 1Heterorhabditis bacteriophora Eukaryota Animals Roundworms 76.9743 - 1 - 1Loa loa Eukaryota Animals Roundworms 96.4054 - 1 - 2Meloidogyne floridensis Eukaryota Animals Roundworms 96.6731 - - - 1Meloidogyne hapla Eukaryota Animals Roundworms 53.013 - - - 1Meloidogyne incognita Eukaryota Animals Roundworms 82.095 - 1 - 1Necator americanus Eukaryota Animals Roundworms 244.075 - 1 - 1Nippostrongylus brasiliensis Eukaryota Animals Roundworms 294.4 - - - 1Oesophagostomum dentatum Eukaryota Animals Roundworms 443.017 - 1 - 1Onchocerca flexuosa Eukaryota Animals Roundworms 86.1755 - 1 - 1Onchocerca ochengi Eukaryota Animals Roundworms 112.154 - - - 1Onchocerca volvulus Eukaryota Animals Roundworms 96.4278 - 1 - 2Oscheius Eukaryota Animals Roundworms 38.3213 - - - 1Panagrellus redivivus Eukaryota Animals Roundworms 65.0931 - - - 1Parascaris equorum Eukaryota Animals Roundworms 185.377 - - - 1Parastrongyloides trichosuri Eukaryota Animals Roundworms 42.487 - - - 1Pristionchus pacificus Eukaryota Animals Roundworms 133.114 - 1 - 1Rhabditophanes Eukaryota Animals Roundworms 47.2679 - - - 1Soboliphyme baturini Eukaryota Animals Roundworms 218.333 - - - 1Steinernema carpocapsae Eukaryota Animals Roundworms 85.9347 - 1 - 1Steinernema feltiae Eukaryota Animals Roundworms 82.5044 - - - 1Steinernema glaseri Eukaryota Animals Roundworms 92.8363 - - - 1Steinernema monticolum Eukaryota Animals Roundworms 89.159 - - - 1Steinernema scapterisci Eukaryota Animals Roundworms 79.6513 - - - 1Strongyloides papillosus Eukaryota Animals Roundworms 60.448 - - - 1Strongyloides stercoralis Eukaryota Animals Roundworms 42.6746 - - - 1Strongylus vulgaris Eukaryota Animals Roundworms 291.096 - 1 - 1Syphacia muris Eukaryota Animals Roundworms 99.0933 - - - 1Toxocara canis Eukaryota Animals Roundworms 317.116 - 1 - 2Trichinella spiralis Eukaryota Animals Roundworms 63.5254 - 1 - 1Trichuris muris Eukaryota Animals Roundworms 84.4053 - - - 1Trichuris suis Eukaryota Animals Roundworms 74.2346 - 1 - 3Trichuris trichiura Eukaryota Animals Roundworms 75.4964 - 1 - 1Wuchereria bancrofti Eukaryota Animals Roundworms 81.5092 - 1 - 2

Genomas de gusanos redondos (Nematoda) en la base de datos del NCBI (61)

Maquinaria Biológica del Parásito

Métodos de Diagnóstico

Identificación deNuevos Fármacos

Relación Huésped-Parásito

Entender su Adaptación Evolutiva

Transfección y Manipulación de Genes

Perspectivas en la Ómica de Parásitos

Moléculas Inmunomoduladoras

Vacunas

Genoma, Transcriptoma,

Proteoma de Parásito

Nature 496, 57–63 (04 April 2013)

ESTs de Taenia solium reportadas en la base de datos del NCBI

Identificación de los procesos biológicos (1)

Gene Ontology

Identificación de los procesos moleculares (2)

Gene Ontology

Vías Metabólicas identificadas (KEGG) en Taenia solium

Trabajos directamente relacionados al estudio de Genoma de Parásitos.

1. Mechanism of attenuation in Babesia vaccine through genomic and transcriptomic analyses.2. Blastocystis – A Parasite Associated with Gastrointestinal Health?3. Neglected Tropical Diseases: Emerging Trematodiases in India: the present scenario.4. Discovery and animal studies of new antigiardiasis drugs.5. Characterization of the complete mitochondrial genome of Setaria digitata from water buffalo in China.6. Transgenesis and functional genomics of schistosomes mediated by mammalian retroviruses.7. Genomics of a colombian strain of Leishmania (Viannia) panamensis.8. A web-based analysis platform for comparative functional genomics and proteomics in eukaryotic pathogens:

Trichomonas vaginalis as an example.9. What helminth genomes have taught us about parasite evolution.10. The genome of the sparganosis tapeworm Spirometra erinaceieuropaei isolated from the biopsy of a migrating

brain lesion.11. The whipworm genome and dual-species transcriptomics of an intimate host-pathogen interaction.12. Comparative genetics: a lens through which to clarify the genomes, the transmission history, and the reproductive

biology of Trichinella spp.13. Using genomics to find targets for a malaria elimination agenda.14. Current perspectives on comparative genomics of three human Taenia tapeworms: Taenia solium, T. saginata and

T. asiatica.15. A proteogenomic approach to investigating snail-schistosome interactions.

ICOPA XIII, México, Agosto 2014

16. An integrated multi-omics approach for studying helminth infections.17. Pan-Nematoda comparative genomics with emphasis on Trichocephalida species.18. Inter and intra species comparative genomics of Entamoeba.19. Schistosome exome sequencing.20. Comparative genomics of parasitic worms. The Helminth Genome Initiative.21. A bird’s eye view on Trichomonas vaginalis vast annotated proteome

ICOPA XIII, México, Agosto 2014

1. Biological functionality and immunogenicity of proteins prohibitin 1 and 2 in Leishmania major.2. Proteomics of the Taenia solium cysticerci in the skeletal muscle and in the central nervous system of naturally

infected pigs.3. A web-based analysis platform for comparative functional genomics and proteomics in eukaryotic pathogens:

Trichomonas vaginalis as an example.4. Expression of recombinant Trypanosome antigens in Leishmania tarentolae.5. Determining the Plasmodium vivax blood stage protein repertoire.6. Virulence factors in wild strain: proteomic map and expression of peroxiredoxins in strain of Trypanosoma cruzi

from the State of Rio de Janeiro, Brazil.7. Comparison of extraction methods for the mass spectrometry analysis of Schistosoma mansoni.8. An antioxidant response is involved in resistance of Giardia duodenalis to albendazole.9. Identification of Novel Diagnostic Serum Biomarkers for Parasitic Diseases by Mass Spectrometry Profiling.10. Proteomic analysis of a virulent, genotype A, canine strain of Giardia duodenalis.

Proteómica

Algunas iniciativas que se presentaron en ICOPA XIII acerca de la genómica de Nemátodos y Tremátodos (1)

Iniciativa para caracterizar 50 parásitos helmintos

Bases de datos de genomas de parásitos, bacterias y virus patógenos del ser humano

Base de Datos del Sanger Institute

Un esfuerzo similar en parásitos protistas patógenos del ser humano

A manera de resumen

• Vivimos en una era de grandes descubrimientos en los enfoques integrales moleculares (òmicas) de los seres vivos.• Los organismos parásitos no son la excepción al respecto; se

encuentran en desarrollo importantes iniciativas de ómica comparada. ICOPA XIII fue un foro donde se analizaron estas iniciativas

• Es de esperarse una serie de nuevas aportaciones en el diagnóstico, tratamiento y prevención de varias enfermedades parasitarias.

¡Gracias!