Investigacin fundacional, Pilar de la Innovacin tecnolgica

Fernando Lpez-Daz, Ph.D., CBL.

Staff Scientist, The Salk Institute for Biological Studies, La Jolla, USA Adjunct Professor Master in Advanced Therapies, UFV, Madrid, SPAIN

Molecular Tumor Board, UCSD Moores Cancer Center, La Jolla, USA Intellectics Inc. San Diego, USA ; CEPIMP Genomics SA, ARGENTINA

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Medicina Genmica

Estrategias genmicas rutinarias en la oncologa clnica

>20-40%tumor

TumorBiopsy

1.DNA/RNASequencing2.Anlisiscien

1. Guia teraputica 2. Investigacin aplicada y clinica -Clinical Trial n=1 3. Drug Discovery. 4. Vigilancia genmica

Pathways Analysis

Incidence

A novel ESR1 mutation from a metastatic prostate carcinoma tumor

Structure Analysis

Laretroalimentacindelconocimientoenbiomedicina:ClinicalPracOceandBiomedicalResearch

Target validation

Drug discovery

Clinical trials

Clinical Practice

Target discovery

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DNA damage

Apoptosis

Pre-malignant cells Primary tumor

>Mutations & selection

Early dissemination

Late dissemination

Blood vessels

Metastases

Normal cells

Cancer Development, Progression and Cellular Stress

Adapted from Klein C., Science 2008

development progression

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2013 Target Discovery:

Technology Dev. & Concept Discovery:

Cancer evolution and Drug Resistance 2014

2013: Target discovery: Control of the stress response in precancerous and cancer cells

2016: using CRISPR technology to produce a assay platform for new compounds screen

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2014: SINGLE CELL RNA SEQUENCING: SELECTIVE / ADAPTIVE EVOLUTION OF CANCER CELL POPULATIONS FOUND IN RNA

Pre-tratamiento Final del tratamiento Post tratamiento

Chemotherapy Drug holiday

time

# of

SN

Vs/

gene

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n

com

plex

ity

cancer cell population RNA heterogeneity

Lopez-Diaz*, Lee* et al. Proc. Natl Acad of Sciences 2014

NEXT: SNP DETECTION IN RNA PLATFORM TO DESIGN NOVEL CONCEPT DRUG SCREENS

S a l k I n s t i t u t e San Diego - USA

N a t i o n a l U n i v e r s i t y o f L i t o r a l Santa Fe - Argentina

Muchas gracias!

Thanks!

Contact: lopezdiaz@salk.edu flopezdiaz@cepimp.com www.salk.edu www.cepimp.com