S (mM)V ( umol/ml.min)

I= 1mM I=10mM I=100mM I=1000mM0.5 2.9 1.1 0.7 0.651 5.6 2.2 1.4 1.35 22.9 10 6.5 6.1

10 37.3 18.3 12.2 11.5

Haciendo inversos 1

1/s 1/V1 1/V2 1/V3 1/V42 0.34482759 0.90909091 1.42857143 1.538461541 0.17857143 0.45454545 0.71428571 0.76923077

0.2 0.04366812 0.1 0.15384615 0.163934430.1 0.02680965 0.05464481 0.08196721 0.08695652

Al ser una inhibicion no competitiva su velocidad vendra expresada de la siguiente manera

v= Vap*S/K+Sv=(V/1+p/Ki)* S/K+S

Para el Inhibidor con una concentracion de P= 1 mM

Ecuacion= y= 0.1674 x + 0.0103

K/Vap= 0.16741/Vap= 0.0103 por tanto Vap= 97.0873786Y=1/V hallo V 1/V=0.0268 V= 37.3X=1/S

Hallo K mi K=Kap

Vap= 97.0873786K/V=0.1674 K= 16.2524272

Hallando la Velocidad

v= Vap*S/K+S

0 0.5 1 1.5 2 2.50

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

f(x) = 0.763396432933783 x + 0.00984375674486548R² = 0.999983784265036f(x) = 0.708052138736578 x + 0.0105246129968356R² = 0.999978129351748

f(x) = 0.449241583840376 x + 0.00894598642657307R² = 0.999961510876662

f(x) = 0.16742979164548 x + 0.0103396190233786R² = 0.999986503252386

Inhibicion No competitiva

Column BLinear (Column B)Column CLinear (Column C)Column DLinear (Column D)Column ELinear (Column E)

v= 36.9822485

Hallando El Ki

Vap= V/(1+(P/Ki))

Despejo Ki

Ki= P/ (V/Vap-1) P= 1 mM

Ki= 0.62387749

Para el Inhibidor con una concentracion de P= 10 mM

Ecuacion: y= 0.4492 X+ 0.0089

K/Vap= 0.44921/Vap= 0.0089

Hallo K y Vap

Vap= 112.359551Kap=K= 25.2359551

Ki= 0.00486575

Podemos concluir que Mi K al ser igual a mi Kap no amument

0 0.5 1 1.5 2 2.50

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

f(x) = 0.763396432933783 x + 0.00984375674486548R² = 0.999983784265036f(x) = 0.708052138736578 x + 0.0105246129968356R² = 0.999978129351748

f(x) = 0.449241583840376 x + 0.00894598642657307R² = 0.999961510876662

f(x) = 0.16742979164548 x + 0.0103396190233786R² = 0.999986503252386

Inhibicion No competitiva

Column BLinear (Column B)Column CLinear (Column C)Column DLinear (Column D)Column ELinear (Column E)

S (mM)V ( umol/ml.min)

I= 1mM I=10mM I=100mM I=1000mM0.5 2.9 1.1 0.7 0.651 5.6 2.2 1.4 1.35 22.9 10 6.5 6.1

10 37.3 18.3 12.2 11.51000

1

S (mM)v ( mM/min)

I= 1mM I=10mM I=100mM I=1000mM0.5 0.0029 0.0011 0.0007 0.000651 0.0056 0.0022 0.0014 0.00135 0.0229 0.01 0.0065 0.0061

10 0.0373 0.0183 0.0122 0.0115

1/s 1/v1 1/v2 1/v3 1/v42 344.827586 909.090909 1428.57143 1538.461541 178.571429 454.545455 714.285714 769.230769

0.2 43.6681223 100 153.846154 163.9344260.1 26.8096515 54.6448087 81.9672131 86.9565217

velocidadHígado Cerebro

1 0.14 0.0852 0.23 0.145 0.39 0.23

10 0.5 0.3

1/s 1/v 1/v1 7.14285714285714 11.7647059

0.5 4.34782608695652 7.142857140.2 2.56410256410256 4.347826090.1 2 3.33333333

Km son iguales

Km/Vmax= 5.72441/Vmaz 1.4377

Vmax= 0.695555400987689Km= 3.98163733741393

cerebro

Km/Vmax 9.33491/Vmax 2.4465

Vmax= 0.40874718986307Km= 3.81561414265277

Concentración de sustrato

(mmoles/ml)

0 0.2 0.4 0.6 0.8 1 1.20

2

4

6

8

10

12

14

f(x) = 9.33491408985955 x + 2.44646927093819

f(x) = 5.72436141202778 x + 1.43773381306656 Column BLinear (Column B)Column CLinear (Column C)

Axis Title

Axis Title

0 0.2 0.4 0.6 0.8 1 1.20

2

4

6

8

10

12

14

f(x) = 9.33491408985955 x + 2.44646927093819

f(x) = 5.72436141202778 x + 1.43773381306656 Column BLinear (Column B)Column CLinear (Column C)

Axis Title

Axis Title

0 0.2 0.4 0.6 0.8 1 1.20

2

4

6

8

10

12

14

f(x) = 9.33491408985955 x + 2.44646927093819

f(x) = 5.72436141202778 x + 1.43773381306656 Column BLinear (Column B)Column CLinear (Column C)

Axis Title

Axis Title