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Principios de Resonancia Magnética

RMN Aplicada a Tecnología de

Alimentos.

Tópicos Especiales en Colorimetría y

Espectrometría.

Prof. Dr. Miguel Martín Landrove

Centro de Física Molecular y Médica, Facultad de Ciencias, Universidad Central

de Venezuela y Centro de Diagnóstico Docente, Las Mercedes, Caracas.

Algunas páginas Web:

• The Basics of NMR, Joseph P. Hornak.

http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm

• NMR Spectroscopy. Principles and Applications, Henry

Zerpa.

http://www.ch.ic.ac.uk/local/organic/nmr.html

• 2D NMR Spectroscopy, Marc Bria, Pierre Watkin, Yves

Plancke.

http://www.univ-lille1.fr/lcom/RMN2D/resum2D_us/index1.htm

El Átomo y el Núcleo Atómico

Protones

Neutrones

Número de electrones = Número de protones = Z

Número de protones + Número de neutrones = A

Z = Número Atómico

A ~ Peso Atómico

Z = Número Atómico

Tabla Periódica

Neutrones

Protones

Espín o Momento

Angular Intrínseco

Momento Magnético

I

||

Isótopos

C12 C13

Z = 6, N = 6

Sin espín

Z = 6, N = 7

Con espín

Desorden en ausencia de campo magnético

El desorden desaparece al aplicar un campo

magnético

S

N

Bo

t/T1

A 3 T1 la recuperación

es de 95 %

Precesión

Precesión

Magnetización en un volumen

Magnetización en un Voxel

Dos Magnetizaciones

La magnetización transversal desaparece rápidamente debido a

inhomogeneidades del campo magnético

Engelsen SB, Jensen MK, Pedersen HT, Nørgaard L, Munck L (2001) NMR-baking and multivariate

prediction of instrumental texture parameters in bread. Journal of Cereal Science, 33, 59–69.

H.T. Pedersen, S. Ablett, D.R. Martin, M.J.D. Mallet, S.B. Engelsen, Application of the NMR-

MOUSE to food emulsions, Journal of Magnetic Resonance 165, 49 (2003).

M.-I. Kuo, S. Gunasekaran, M. Johnson, C. Chen, Nuclear magnetic resonance study of water

mobility in pasta filata and non-pasta filata mozzarella, J. Dairy Sci. 84, 1950 (2001).

M.-I. Kuo, S. Gunasekaran, M. Johnson, C. Chen, Nuclear magnetic resonance study of water

mobility in pasta filata and non-pasta filata mozzarella, J. Dairy Sci. 84, 1950 (2001).

E. Leal, M. Martín-Landrove, Z. González, Estudio de almidones nativos y almidones

modificados mediante la resonancia magnética nuclear, Memorias IV Congreso Venezolano de

Química 077, 212 (1999).

Secuencia de Inversión-Recuperación

M.-I. Kuo, S. Gunasekaran, M. Johnson, C. Chen, Nuclear magnetic resonance study of water

mobility in pasta filata and non-pasta filata mozzarella, J. Dairy Sci. 84, 1950 (2001).

Difusión aparente o limitada

Difusión libre

Dl > Dap

G. Liger-Belair, E. Prost, M. Parmentier, P. Jeandet, J.-M. Nuzillard, Diffusion coefficient of

CO2 molecules as determined by 13C NMR in various carbonated beverages, Journal of

Agricultural and Food Chemistry 51, 7560 (2003).

B B0

B0

G. Le Gall, I.J. Colquhoun, M. Defernez, Metabolite profiling using 1H NMR spectroscopy for

quality assessment of green tea, Camellia cinensis (L.), Journal of Agricultural and Food

Chemistry 52, 692 (2004).

G. Le Gall, I.J. Colquhoun, M. Defernez, Metabolite profiling using 1H NMR spectroscopy for

quality assessment of green tea, Camellia cinensis (L.), Journal of Agricultural and Food

Chemistry 52, 692 (2004).

I.F. Duarte, A. Barros, C. Almeida, M. Spraul, A.M. Gil, Multivariate analysis of NMR and FTIR

data as a potential tool for the quality control of beer, Journal of Agricultural and Food

Chemistry 52, 1031 (2004).

W. Dolata, E. Piotrowska, J. Wajdzik, J. Tritt-Goc, The use of the MRI technique in the evaluation of water

Distribution in tumbled porcine muscle, Meat Science 67, 25 (2004).

H.C. Bertram, A.K. Wittaker, W.R. Shorthose, H.K. Andersen, A.H. Karlsson, Water

characteristics in cooked beef as influenced by ageing and high-pressure treatment - an NMR

micro imaging study, Meat Science 66, 301 (2004).

G. Galed, M.E. Fernández-Valle, A. Martínez, A. Heras, Application of MRI to monitor the

process of ripening and decay in citrus treated with chitosan solutions, Magnetic Resonance

Imaging 22, 127 (2004).

J. Lammertyn, T. Dresselaers, P. Van Hecke, P. Jansóck, M. Wevers, B.M. Nicolaï, Analysis of

the time course of core breakdown in ‘Conference’ pears by means of MRI and X-Ray CT,

Postharvest Biology and Technology 29, 19 (2003).

J. Lammertyn, T. Dresselaers, P. Van Hecke, P. Jansóck, M. Wevers, B.M. Nicolaï, Analysis of

the time course of core breakdown in ‘Conference’ pears by means of MRI and X-Ray CT,

Postharvest Biology and Technology 29, 19 (2003).

L. Foucat, R.G. Taylor, R. Labas, J.P. Renou, Characterization of frozen fish by NMR imaging

and histology, August (2001).

STUDIES OF PHYSICAL STATE OF WATER IN MAIZE FROM NORTHEAST CHINA (CV. HUANGMO

417) DURING DRYING AT SUBZERO TEMPERATURES, C. Borompichaichartkul, G. Moran, G.S.

Srzednicki and R.H. Driscoll Drying’ 2002-Proceedings of the 13th International Drying Symposium

(IDS’ 2002) Beijing, China, 27-30 August’ 2002, vol. B, pp. 1325

STUDIES OF PHYSICAL STATE OF WATER IN MAIZE FROM NORTHEAST CHINA (CV. HUANGMO

417) DURING DRYING AT SUBZERO TEMPERATURES, C. Borompichaichartkul, G. Moran, G.S.

Srzednicki and R.H. Driscoll Drying’ 2002-Proceedings of the 13th International Drying Symposium

(IDS’ 2002) Beijing, China, 27-30 August’ 2002, vol. B, pp. 1325